Development of a reverse transcription (RT) polymerase chain reaction (PCR) method for the detection of human norovirus in bivalve molluscs

Noroviruses are significant seafood-borne pathogens, commonly associated with the consumption of filter feeding bivalve molluscs. Here, we report the development of a reverse transcription polymerase chain reaction (RT-PCR) method using primers based on the RNA-dependent RNA polymerase gene of norov...

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Autores principales:	Manjusha Lekshmi, Sanath H. Kumar, Kooloth Valappil Rajendran, Binaya Bhusan Nayak
Formato:	article
Lenguaje:	EN
Publicado:	IWA Publishing 2021
Materias:	enteric virus norovirus gii rt-pcr seafood shellfish Environmental technology. Sanitary engineering TD1-1066
Acceso en línea:	https://doaj.org/article/d9134125fe9d4facb15729fa757e4c97
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spelling	oai:doaj.org-article:d9134125fe9d4facb15729fa757e4c972021-11-06T10:50:49ZDevelopment of a reverse transcription (RT) polymerase chain reaction (PCR) method for the detection of human norovirus in bivalve molluscs0273-12231996-973210.2166/wst.2021.048https://doaj.org/article/d9134125fe9d4facb15729fa757e4c972021-03-01T00:00:00Zhttp://wst.iwaponline.com/content/83/5/1103https://doaj.org/toc/0273-1223https://doaj.org/toc/1996-9732Noroviruses are significant seafood-borne pathogens, commonly associated with the consumption of filter feeding bivalve molluscs. Here, we report the development of a reverse transcription polymerase chain reaction (RT-PCR) method using primers based on the RNA-dependent RNA polymerase gene of norovirus genogroup II (NoV GII). Samples of bivalves were processed for the concentration of virus and extraction of RNA, followed by reverse transcription PCR. A total of 50 molluscan shellfish samples were analyzed, of which 16 samples yielded positive amplifications of norovirus nucleic acid. The PCR method described here, involving a single set of primers, is useful for rapid screening of shellfish for NoV GII.Manjusha LekshmiSanath H. KumarKooloth Valappil RajendranBinaya Bhusan NayakIWA Publishingarticleenteric virusnorovirus giirt-pcrseafoodshellfishEnvironmental technology. Sanitary engineeringTD1-1066ENWater Science and Technology, Vol 83, Iss 5, Pp 1103-1107 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	enteric virus norovirus gii rt-pcr seafood shellfish Environmental technology. Sanitary engineering TD1-1066
spellingShingle	enteric virus norovirus gii rt-pcr seafood shellfish Environmental technology. Sanitary engineering TD1-1066 Manjusha Lekshmi Sanath H. Kumar Kooloth Valappil Rajendran Binaya Bhusan Nayak Development of a reverse transcription (RT) polymerase chain reaction (PCR) method for the detection of human norovirus in bivalve molluscs
description	Noroviruses are significant seafood-borne pathogens, commonly associated with the consumption of filter feeding bivalve molluscs. Here, we report the development of a reverse transcription polymerase chain reaction (RT-PCR) method using primers based on the RNA-dependent RNA polymerase gene of norovirus genogroup II (NoV GII). Samples of bivalves were processed for the concentration of virus and extraction of RNA, followed by reverse transcription PCR. A total of 50 molluscan shellfish samples were analyzed, of which 16 samples yielded positive amplifications of norovirus nucleic acid. The PCR method described here, involving a single set of primers, is useful for rapid screening of shellfish for NoV GII.
format	article
author	Manjusha Lekshmi Sanath H. Kumar Kooloth Valappil Rajendran Binaya Bhusan Nayak
author_facet	Manjusha Lekshmi Sanath H. Kumar Kooloth Valappil Rajendran Binaya Bhusan Nayak
author_sort	Manjusha Lekshmi
title	Development of a reverse transcription (RT) polymerase chain reaction (PCR) method for the detection of human norovirus in bivalve molluscs
title_short	Development of a reverse transcription (RT) polymerase chain reaction (PCR) method for the detection of human norovirus in bivalve molluscs
title_full	Development of a reverse transcription (RT) polymerase chain reaction (PCR) method for the detection of human norovirus in bivalve molluscs
title_fullStr	Development of a reverse transcription (RT) polymerase chain reaction (PCR) method for the detection of human norovirus in bivalve molluscs
title_full_unstemmed	Development of a reverse transcription (RT) polymerase chain reaction (PCR) method for the detection of human norovirus in bivalve molluscs
title_sort	development of a reverse transcription (rt) polymerase chain reaction (pcr) method for the detection of human norovirus in bivalve molluscs
publisher	IWA Publishing
publishDate	2021
url	https://doaj.org/article/d9134125fe9d4facb15729fa757e4c97
work_keys_str_mv	AT manjushalekshmi developmentofareversetranscriptionrtpolymerasechainreactionpcrmethodforthedetectionofhumannorovirusinbivalvemolluscs AT sanathhkumar developmentofareversetranscriptionrtpolymerasechainreactionpcrmethodforthedetectionofhumannorovirusinbivalvemolluscs AT koolothvalappilrajendran developmentofareversetranscriptionrtpolymerasechainreactionpcrmethodforthedetectionofhumannorovirusinbivalvemolluscs AT binayabhusannayak developmentofareversetranscriptionrtpolymerasechainreactionpcrmethodforthedetectionofhumannorovirusinbivalvemolluscs
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Development of a reverse transcription (RT) polymerase chain reaction (PCR) method for the detection of human norovirus in bivalve molluscs

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