Diversity of selected Lupinus angustifolius L. genotypes at the phenotypic and DNA level with respect to microscopic seed coat structure and thickness.

The paper investigates seed coat characteristics (as a percentage of overall seed diameter) in Lupinus angustifolius L., a potential forage crop. In the study ten L. angustifolius genotypes, including three Polish cultivars, two Australian cultivars, three mutants originated from cv. 'Emir'...

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Autores principales:	Jon Clements, Renata Galek, Bartosz Kozak, Dariusz Jan Michalczyk, Agnieszka Iwona Piotrowicz-Cieślak, Ewa Sawicka-Sienkiewicz, Stanislaw Stawiński, Dariusz Zalewski
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d91ef3df4dca43ef8982468546d246f8
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spelling	oai:doaj.org-article:d91ef3df4dca43ef8982468546d246f82021-11-25T06:04:50ZDiversity of selected Lupinus angustifolius L. genotypes at the phenotypic and DNA level with respect to microscopic seed coat structure and thickness.1932-620310.1371/journal.pone.0102874https://doaj.org/article/d91ef3df4dca43ef8982468546d246f82014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25119983/?tool=EBIhttps://doaj.org/toc/1932-6203The paper investigates seed coat characteristics (as a percentage of overall seed diameter) in Lupinus angustifolius L., a potential forage crop. In the study ten L. angustifolius genotypes, including three Polish cultivars, two Australian cultivars, three mutants originated from cv. 'Emir', and one Belarusian and one Australian breeding line were evaluated. The highest seed coat percentage was recorded in cultivars 'Sonet' and 'Emir'. The lowest seed coat thickness percentage (below 20%) was noted for breeding lines 11257-19, LAG24 and cultivar 'Zeus' (17.87%, 18.91% 19.60%, respectively). Despite having low seed weight, the Australian line no. 11257-19 was characterized by a desirable proportion of seed coat to the weight of seeds. In general, estimation of the correlation coefficient indicated a tendency that larger seeds had thinner coats. Scanning Electron Microscopy images showed low variation of seed coat sculpture and the top of seeds covered with a cuticle. Most of the studied genotypes were characterized by a cristatepapillate seed coat surface, formed by elongated polygonal cells. Only breeding line no. 11267-19 had a different shape of the cells building the surface layer of the coat. In order to illustrate genetic diversity among the genotypes tested, 24 ISSR primers were used. They generated a total of 161 polymorphic amplification products in 10 evaluated narrow-leaved lupin genotypes.Jon ClementsRenata GalekBartosz KozakDariusz Jan MichalczykAgnieszka Iwona Piotrowicz-CieślakEwa Sawicka-SienkiewiczStanislaw StawińskiDariusz ZalewskiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 8, p e102874 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jon Clements Renata Galek Bartosz Kozak Dariusz Jan Michalczyk Agnieszka Iwona Piotrowicz-Cieślak Ewa Sawicka-Sienkiewicz Stanislaw Stawiński Dariusz Zalewski Diversity of selected Lupinus angustifolius L. genotypes at the phenotypic and DNA level with respect to microscopic seed coat structure and thickness.
description	The paper investigates seed coat characteristics (as a percentage of overall seed diameter) in Lupinus angustifolius L., a potential forage crop. In the study ten L. angustifolius genotypes, including three Polish cultivars, two Australian cultivars, three mutants originated from cv. 'Emir', and one Belarusian and one Australian breeding line were evaluated. The highest seed coat percentage was recorded in cultivars 'Sonet' and 'Emir'. The lowest seed coat thickness percentage (below 20%) was noted for breeding lines 11257-19, LAG24 and cultivar 'Zeus' (17.87%, 18.91% 19.60%, respectively). Despite having low seed weight, the Australian line no. 11257-19 was characterized by a desirable proportion of seed coat to the weight of seeds. In general, estimation of the correlation coefficient indicated a tendency that larger seeds had thinner coats. Scanning Electron Microscopy images showed low variation of seed coat sculpture and the top of seeds covered with a cuticle. Most of the studied genotypes were characterized by a cristatepapillate seed coat surface, formed by elongated polygonal cells. Only breeding line no. 11267-19 had a different shape of the cells building the surface layer of the coat. In order to illustrate genetic diversity among the genotypes tested, 24 ISSR primers were used. They generated a total of 161 polymorphic amplification products in 10 evaluated narrow-leaved lupin genotypes.
format	article
author	Jon Clements Renata Galek Bartosz Kozak Dariusz Jan Michalczyk Agnieszka Iwona Piotrowicz-Cieślak Ewa Sawicka-Sienkiewicz Stanislaw Stawiński Dariusz Zalewski
author_facet	Jon Clements Renata Galek Bartosz Kozak Dariusz Jan Michalczyk Agnieszka Iwona Piotrowicz-Cieślak Ewa Sawicka-Sienkiewicz Stanislaw Stawiński Dariusz Zalewski
author_sort	Jon Clements
title	Diversity of selected Lupinus angustifolius L. genotypes at the phenotypic and DNA level with respect to microscopic seed coat structure and thickness.
title_short	Diversity of selected Lupinus angustifolius L. genotypes at the phenotypic and DNA level with respect to microscopic seed coat structure and thickness.
title_full	Diversity of selected Lupinus angustifolius L. genotypes at the phenotypic and DNA level with respect to microscopic seed coat structure and thickness.
title_fullStr	Diversity of selected Lupinus angustifolius L. genotypes at the phenotypic and DNA level with respect to microscopic seed coat structure and thickness.
title_full_unstemmed	Diversity of selected Lupinus angustifolius L. genotypes at the phenotypic and DNA level with respect to microscopic seed coat structure and thickness.
title_sort	diversity of selected lupinus angustifolius l. genotypes at the phenotypic and dna level with respect to microscopic seed coat structure and thickness.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/d91ef3df4dca43ef8982468546d246f8
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Diversity of selected Lupinus angustifolius L. genotypes at the phenotypic and DNA level with respect to microscopic seed coat structure and thickness.

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