Population Structure, Antibiotic Resistance, and Uropathogenicity of <named-content content-type="genus-species">Klebsiella variicola</named-content>

ABSTRACT Klebsiella variicola is a member of the Klebsiella genus and often misidentified as Klebsiella pneumoniae or Klebsiella quasipneumoniae. The importance of K. pneumoniae human infections has been known; however, a dearth of relative knowledge exists for K. variicola. Despite its growing clin...

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Autores principales:	Robert F. Potter, William Lainhart, Joy Twentyman, Meghan A. Wallace, Bin Wang, Carey-Ann D. Burnham, David A. Rosen, Gautam Dantas
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	emerging pathogens Klebsiella antibiotic resistance microbial genomics urinary tract infection Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/d92139eb56c4425d9ee36306771b7e1d
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spelling	oai:doaj.org-article:d92139eb56c4425d9ee36306771b7e1d2021-11-15T15:52:19ZPopulation Structure, Antibiotic Resistance, and Uropathogenicity of <named-content content-type="genus-species">Klebsiella variicola</named-content>10.1128/mBio.02481-182150-7511https://doaj.org/article/d92139eb56c4425d9ee36306771b7e1d2018-12-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.02481-18https://doaj.org/toc/2150-7511ABSTRACT Klebsiella variicola is a member of the Klebsiella genus and often misidentified as Klebsiella pneumoniae or Klebsiella quasipneumoniae. The importance of K. pneumoniae human infections has been known; however, a dearth of relative knowledge exists for K. variicola. Despite its growing clinical importance, comprehensive analyses of K. variicola population structure and mechanistic investigations of virulence factors and antibiotic resistance genes have not yet been performed. To address this, we utilized in silico, in vitro, and in vivo methods to study a cohort of K. variicola isolates and genomes. We found that the K. variicola population structure has two distant lineages composed of two and 143 genomes, respectively. Ten of 145 K. variicola genomes harbored carbapenem resistance genes, and 6/145 contained complete virulence operons. While the β-lactam blaLEN and quinolone oqxAB antibiotic resistance genes were generally conserved within our institutional cohort, unexpectedly 11 isolates were nonresistant to the β-lactam ampicillin and only one isolate was nonsusceptible to the quinolone ciprofloxacin. K. variicola isolates have variation in ability to cause urinary tract infections in a newly developed murine model, but importantly a strain had statistically significant higher bladder CFU than the model uropathogenic K. pneumoniae strain TOP52. Type 1 pilus and genomic identification of altered fim operon structure were associated with differences in bladder CFU for the tested strains. Nine newly reported types of pilus genes were discovered in the K. variicola pan-genome, including the first identified P-pilus in Klebsiella spp. IMPORTANCE Infections caused by antibiotic-resistant bacterial pathogens are a growing public health threat. Understanding of pathogen relatedness and biology is imperative for tracking outbreaks and developing therapeutics. Here, we detail the phylogenetic structure of 145 K. variicola genomes from different continents. Our results have important clinical ramifications as high-risk antibiotic resistance genes are present in K. variicola genomes from a variety of geographic locations and as we demonstrate that K. variicola clinical isolates can establish higher bladder titers than K. pneumoniae. Differential presence of these pilus genes inK. variicola isolates may indicate adaption for specific environmental niches. Therefore, due to the potential of multidrug resistance and pathogenic efficacy, identification of K. variicola and K. pneumoniae to a species level should be performed to optimally improve patient outcomes during infection. This work provides a foundation for our improved understanding of K. variicola biology and pathogenesis.Robert F. PotterWilliam LainhartJoy TwentymanMeghan A. WallaceBin WangCarey-Ann D. BurnhamDavid A. RosenGautam DantasAmerican Society for Microbiologyarticleemerging pathogensKlebsiellaantibiotic resistancemicrobial genomicsurinary tract infectionMicrobiologyQR1-502ENmBio, Vol 9, Iss 6 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	emerging pathogens Klebsiella antibiotic resistance microbial genomics urinary tract infection Microbiology QR1-502
spellingShingle	emerging pathogens Klebsiella antibiotic resistance microbial genomics urinary tract infection Microbiology QR1-502 Robert F. Potter William Lainhart Joy Twentyman Meghan A. Wallace Bin Wang Carey-Ann D. Burnham David A. Rosen Gautam Dantas Population Structure, Antibiotic Resistance, and Uropathogenicity of <named-content content-type="genus-species">Klebsiella variicola</named-content>
description	ABSTRACT Klebsiella variicola is a member of the Klebsiella genus and often misidentified as Klebsiella pneumoniae or Klebsiella quasipneumoniae. The importance of K. pneumoniae human infections has been known; however, a dearth of relative knowledge exists for K. variicola. Despite its growing clinical importance, comprehensive analyses of K. variicola population structure and mechanistic investigations of virulence factors and antibiotic resistance genes have not yet been performed. To address this, we utilized in silico, in vitro, and in vivo methods to study a cohort of K. variicola isolates and genomes. We found that the K. variicola population structure has two distant lineages composed of two and 143 genomes, respectively. Ten of 145 K. variicola genomes harbored carbapenem resistance genes, and 6/145 contained complete virulence operons. While the β-lactam blaLEN and quinolone oqxAB antibiotic resistance genes were generally conserved within our institutional cohort, unexpectedly 11 isolates were nonresistant to the β-lactam ampicillin and only one isolate was nonsusceptible to the quinolone ciprofloxacin. K. variicola isolates have variation in ability to cause urinary tract infections in a newly developed murine model, but importantly a strain had statistically significant higher bladder CFU than the model uropathogenic K. pneumoniae strain TOP52. Type 1 pilus and genomic identification of altered fim operon structure were associated with differences in bladder CFU for the tested strains. Nine newly reported types of pilus genes were discovered in the K. variicola pan-genome, including the first identified P-pilus in Klebsiella spp. IMPORTANCE Infections caused by antibiotic-resistant bacterial pathogens are a growing public health threat. Understanding of pathogen relatedness and biology is imperative for tracking outbreaks and developing therapeutics. Here, we detail the phylogenetic structure of 145 K. variicola genomes from different continents. Our results have important clinical ramifications as high-risk antibiotic resistance genes are present in K. variicola genomes from a variety of geographic locations and as we demonstrate that K. variicola clinical isolates can establish higher bladder titers than K. pneumoniae. Differential presence of these pilus genes inK. variicola isolates may indicate adaption for specific environmental niches. Therefore, due to the potential of multidrug resistance and pathogenic efficacy, identification of K. variicola and K. pneumoniae to a species level should be performed to optimally improve patient outcomes during infection. This work provides a foundation for our improved understanding of K. variicola biology and pathogenesis.
format	article
author	Robert F. Potter William Lainhart Joy Twentyman Meghan A. Wallace Bin Wang Carey-Ann D. Burnham David A. Rosen Gautam Dantas
author_facet	Robert F. Potter William Lainhart Joy Twentyman Meghan A. Wallace Bin Wang Carey-Ann D. Burnham David A. Rosen Gautam Dantas
author_sort	Robert F. Potter
title	Population Structure, Antibiotic Resistance, and Uropathogenicity of <named-content content-type="genus-species">Klebsiella variicola</named-content>
title_short	Population Structure, Antibiotic Resistance, and Uropathogenicity of <named-content content-type="genus-species">Klebsiella variicola</named-content>
title_full	Population Structure, Antibiotic Resistance, and Uropathogenicity of <named-content content-type="genus-species">Klebsiella variicola</named-content>
title_fullStr	Population Structure, Antibiotic Resistance, and Uropathogenicity of <named-content content-type="genus-species">Klebsiella variicola</named-content>
title_full_unstemmed	Population Structure, Antibiotic Resistance, and Uropathogenicity of <named-content content-type="genus-species">Klebsiella variicola</named-content>
title_sort	population structure, antibiotic resistance, and uropathogenicity of <named-content content-type="genus-species">klebsiella variicola</named-content>
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/d92139eb56c4425d9ee36306771b7e1d
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Population Structure, Antibiotic Resistance, and Uropathogenicity of <named-content content-type="genus-species">Klebsiella variicola</named-content>

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