Platelet-functionalized three-dimensional poly-ε-caprolactone fibrous scaffold prepared using centrifugal spinning for delivery of growth factors

Platelet-functionalized three-dimensional poly-ε-caprolactone fibrous scaffold prepared using centrifugal spinning for delivery of growth factors

Michala Rampichová,1,2 Matej Buzgo,1 Andrea Míčková,1,2 Karolína Vocetková,2 Vera Sovková,2 Vera Lukášová,2 Eva Filová,2 Franco Rustichelli,2 Evžen Amler1,2 1Indoor Environmental Quality, U...

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Autores principales: Rampichová M, Buzgo M, Míčková A, Vocetková K, Sovková V, Lukášová V, Filová E, Rustichelli F, Amler E
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2017
Materias:
centrifugal spinning
3D scaffold
platelets
growth factors
cytokines
PCL
Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/d927638a914c48dabe99911ccaf14384
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spelling oai:doaj.org-article:d927638a914c48dabe99911ccaf143842021-12-02T00:31:21ZPlatelet-functionalized three-dimensional poly-ε-caprolactone fibrous scaffold prepared using centrifugal spinning for delivery of growth factors1178-2013https://doaj.org/article/d927638a914c48dabe99911ccaf143842017-01-01T00:00:00Zhttps://www.dovepress.com/platelet-functionalized-three-dimensional-poly-epsilon-caprolactone-fi-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Michala Rampichová,1,2 Matej Buzgo,1 Andrea Míčková,1,2 Karolína Vocetková,2 Vera Sovková,2 Vera Lukášová,2 Eva Filová,2 Franco Rustichelli,2 Evžen Amler1,2 1Indoor Environmental Quality, University Center for Energy Efficient Buildings, Czech Technical University in Prague, Buštehrad, 2Laboratory of Tissue Engineering, Institute of Experimental Medicine, Czech Academy of Sciences, Prague, Czech Republic Abstract: Bone and cartilage are tissues of a three-dimensional (3D) nature. Therefore, scaffolds for their regeneration should support cell infiltration and growth in all 3 dimensions. To fulfill such a requirement, the materials should possess large, open pores. Centrifugal spinning is a simple method for producing 3D fibrous scaffolds with large and interconnected pores. However, the process of bone regeneration is rather complex and requires additional stimulation by active molecules. In the current study, we introduced a simple composite scaffold based on platelet adhesion to poly-ε-caprolactone 3D fibers. Platelets were used as a natural source of growth factors and cytokines active in the tissue repair process. By immobilization in the fibrous scaffolds, their bioavailability was prolonged. The biological evaluation of the proposed system in the MG-63 model showed improved metabolic activity, proliferation and alkaline phosphatase activity in comparison to nonfunctionalized fibrous scaffold. In addition, the response of cells was dose dependent with improved biocompatibility with increasing platelet concentration. The results demonstrated the suitability of the system for bone tissue. Keywords: centrifugal spinning, 3D scaffold, platelets, growth factors, cytokines, PCLRampichová MBuzgo MMíčková AVocetková KSovková VLukášová VFilová ERustichelli FAmler EDove Medical Pressarticlecentrifugal spinning3D scaffoldplateletsgrowth factorscytokinesPCLMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 347-361 (2017)
institution DOAJ
collection DOAJ
language EN
topic centrifugal spinning
3D scaffold
platelets
growth factors
cytokines
PCL
Medicine (General)
R5-920
spellingShingle centrifugal spinning
3D scaffold
platelets
growth factors
cytokines
PCL
Medicine (General)
R5-920
Rampichová M
Buzgo M
Míčková A
Vocetková K
Sovková V
Lukášová V
Filová E
Rustichelli F
Amler E
Platelet-functionalized three-dimensional poly-ε-caprolactone fibrous scaffold prepared using centrifugal spinning for delivery of growth factors
description Michala Rampichová,1,2 Matej Buzgo,1 Andrea Míčková,1,2 Karolína Vocetková,2 Vera Sovková,2 Vera Lukášová,2 Eva Filová,2 Franco Rustichelli,2 Evžen Amler1,2 1Indoor Environmental Quality, University Center for Energy Efficient Buildings, Czech Technical University in Prague, Buštehrad, 2Laboratory of Tissue Engineering, Institute of Experimental Medicine, Czech Academy of Sciences, Prague, Czech Republic Abstract: Bone and cartilage are tissues of a three-dimensional (3D) nature. Therefore, scaffolds for their regeneration should support cell infiltration and growth in all 3 dimensions. To fulfill such a requirement, the materials should possess large, open pores. Centrifugal spinning is a simple method for producing 3D fibrous scaffolds with large and interconnected pores. However, the process of bone regeneration is rather complex and requires additional stimulation by active molecules. In the current study, we introduced a simple composite scaffold based on platelet adhesion to poly-ε-caprolactone 3D fibers. Platelets were used as a natural source of growth factors and cytokines active in the tissue repair process. By immobilization in the fibrous scaffolds, their bioavailability was prolonged. The biological evaluation of the proposed system in the MG-63 model showed improved metabolic activity, proliferation and alkaline phosphatase activity in comparison to nonfunctionalized fibrous scaffold. In addition, the response of cells was dose dependent with improved biocompatibility with increasing platelet concentration. The results demonstrated the suitability of the system for bone tissue. Keywords: centrifugal spinning, 3D scaffold, platelets, growth factors, cytokines, PCL
format article
author Rampichová M
Buzgo M
Míčková A
Vocetková K
Sovková V
Lukášová V
Filová E
Rustichelli F
Amler E
author_facet Rampichová M
Buzgo M
Míčková A
Vocetková K
Sovková V
Lukášová V
Filová E
Rustichelli F
Amler E
author_sort Rampichová M
title Platelet-functionalized three-dimensional poly-ε-caprolactone fibrous scaffold prepared using centrifugal spinning for delivery of growth factors
title_short Platelet-functionalized three-dimensional poly-ε-caprolactone fibrous scaffold prepared using centrifugal spinning for delivery of growth factors
title_full Platelet-functionalized three-dimensional poly-ε-caprolactone fibrous scaffold prepared using centrifugal spinning for delivery of growth factors
title_fullStr Platelet-functionalized three-dimensional poly-ε-caprolactone fibrous scaffold prepared using centrifugal spinning for delivery of growth factors
title_full_unstemmed Platelet-functionalized three-dimensional poly-ε-caprolactone fibrous scaffold prepared using centrifugal spinning for delivery of growth factors
title_sort platelet-functionalized three-dimensional poly-ε-caprolactone fibrous scaffold prepared using centrifugal spinning for delivery of growth factors
publisher Dove Medical Press
publishDate 2017
url https://doaj.org/article/d927638a914c48dabe99911ccaf14384
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