Periconceptional nutrition with spineless cactus (Opuntia ficus-indica) improves metabolomic profiles and pregnancy outcomes in sheep

Abstract We tested whether periconceptional nutrition with cladodes from the cactus, Opuntia ficus-indica, with or without protein-enrichment, improved the metabolomic profile and reproductive outcomes of adult female sheep. Sixty Rambouillet ewes of similar body weight were randomly allocated among...

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Autores principales:	César A. Rosales-Nieto, Maribel Rodríguez-Aguilar, Francisco Santiago-Hernandez, Venancio Cuevas-Reyes, Manuel J. Flores-Najera, Juan M. Vázquez-García, Jorge Urrutia-Morales, Morteza Hosseini Ghaffari, César A. Meza-Herrera, Antonio González-Bulnes, Graeme B. Martin
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d93badc26da947e7a91694da19b317e8
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spelling	oai:doaj.org-article:d93badc26da947e7a91694da19b317e82021-12-02T13:26:37ZPericonceptional nutrition with spineless cactus (Opuntia ficus-indica) improves metabolomic profiles and pregnancy outcomes in sheep10.1038/s41598-021-86653-w2045-2322https://doaj.org/article/d93badc26da947e7a91694da19b317e82021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86653-whttps://doaj.org/toc/2045-2322Abstract We tested whether periconceptional nutrition with cladodes from the cactus, Opuntia ficus-indica, with or without protein-enrichment, improved the metabolomic profile and reproductive outcomes of adult female sheep. Sixty Rambouillet ewes of similar body weight were randomly allocated among three nutritional treatments that were fed during the breeding period (34 days): Control (Control; n = 20), Opuntia (Opuntia; n = 20) and protein-enriched Opuntia (E-Opuntia; n = 20). There were no effects of treatment on body weight but assessment of urine samples indicated that, for 76 metabolites, the Control and Opuntia groups differed completely (P < 0.05), whereas there was overlap between the Control and E-Opuntia groups. It appears that, in Opuntia-fed and Control-fed sheep, different functional groups are activated leading to changes in the metabolism of glucose, tyrosine, methane, and glycerolipids. Fertility and reproductive rate tended to be higher in the Opuntia (70% and 95%) and E-Opuntia (90% and 110%) groups than in the Control (55% and 65%), and an orthogonal contrast revealed the difference between Control and Opuntia to be significant for both reproductive variables (P < 0.05). We conclude that nutritional supplementation with Opuntia cladodes, with or without protein enrichment, increased fertility rate and reproductive rate of female sheep, without any accompanying increases in body weight. Our observations suggest that the reproductive responses to Opuntia do not simply reflect a response to good nutrition, but might be caused by specific metabolites/metabolomic pathways, perhaps by an activation of the metabolism of glucose, methane, tyrosine and glycerolipids. There are few reports relating these metabolomic compounds with the metabolism of the sheep, let alone with reproductive efficiency. The novelty of these discoveries suggests that we need further research into the mechanisms through which nutrition affects the reproductive system.César A. Rosales-NietoMaribel Rodríguez-AguilarFrancisco Santiago-HernandezVenancio Cuevas-ReyesManuel J. Flores-NajeraJuan M. Vázquez-GarcíaJorge Urrutia-MoralesMorteza Hosseini GhaffariCésar A. Meza-HerreraAntonio González-BulnesGraeme B. MartinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q César A. Rosales-Nieto Maribel Rodríguez-Aguilar Francisco Santiago-Hernandez Venancio Cuevas-Reyes Manuel J. Flores-Najera Juan M. Vázquez-García Jorge Urrutia-Morales Morteza Hosseini Ghaffari César A. Meza-Herrera Antonio González-Bulnes Graeme B. Martin Periconceptional nutrition with spineless cactus (Opuntia ficus-indica) improves metabolomic profiles and pregnancy outcomes in sheep
description	Abstract We tested whether periconceptional nutrition with cladodes from the cactus, Opuntia ficus-indica, with or without protein-enrichment, improved the metabolomic profile and reproductive outcomes of adult female sheep. Sixty Rambouillet ewes of similar body weight were randomly allocated among three nutritional treatments that were fed during the breeding period (34 days): Control (Control; n = 20), Opuntia (Opuntia; n = 20) and protein-enriched Opuntia (E-Opuntia; n = 20). There were no effects of treatment on body weight but assessment of urine samples indicated that, for 76 metabolites, the Control and Opuntia groups differed completely (P < 0.05), whereas there was overlap between the Control and E-Opuntia groups. It appears that, in Opuntia-fed and Control-fed sheep, different functional groups are activated leading to changes in the metabolism of glucose, tyrosine, methane, and glycerolipids. Fertility and reproductive rate tended to be higher in the Opuntia (70% and 95%) and E-Opuntia (90% and 110%) groups than in the Control (55% and 65%), and an orthogonal contrast revealed the difference between Control and Opuntia to be significant for both reproductive variables (P < 0.05). We conclude that nutritional supplementation with Opuntia cladodes, with or without protein enrichment, increased fertility rate and reproductive rate of female sheep, without any accompanying increases in body weight. Our observations suggest that the reproductive responses to Opuntia do not simply reflect a response to good nutrition, but might be caused by specific metabolites/metabolomic pathways, perhaps by an activation of the metabolism of glucose, methane, tyrosine and glycerolipids. There are few reports relating these metabolomic compounds with the metabolism of the sheep, let alone with reproductive efficiency. The novelty of these discoveries suggests that we need further research into the mechanisms through which nutrition affects the reproductive system.
format	article
author	César A. Rosales-Nieto Maribel Rodríguez-Aguilar Francisco Santiago-Hernandez Venancio Cuevas-Reyes Manuel J. Flores-Najera Juan M. Vázquez-García Jorge Urrutia-Morales Morteza Hosseini Ghaffari César A. Meza-Herrera Antonio González-Bulnes Graeme B. Martin
author_facet	César A. Rosales-Nieto Maribel Rodríguez-Aguilar Francisco Santiago-Hernandez Venancio Cuevas-Reyes Manuel J. Flores-Najera Juan M. Vázquez-García Jorge Urrutia-Morales Morteza Hosseini Ghaffari César A. Meza-Herrera Antonio González-Bulnes Graeme B. Martin
author_sort	César A. Rosales-Nieto
title	Periconceptional nutrition with spineless cactus (Opuntia ficus-indica) improves metabolomic profiles and pregnancy outcomes in sheep
title_short	Periconceptional nutrition with spineless cactus (Opuntia ficus-indica) improves metabolomic profiles and pregnancy outcomes in sheep
title_full	Periconceptional nutrition with spineless cactus (Opuntia ficus-indica) improves metabolomic profiles and pregnancy outcomes in sheep
title_fullStr	Periconceptional nutrition with spineless cactus (Opuntia ficus-indica) improves metabolomic profiles and pregnancy outcomes in sheep
title_full_unstemmed	Periconceptional nutrition with spineless cactus (Opuntia ficus-indica) improves metabolomic profiles and pregnancy outcomes in sheep
title_sort	periconceptional nutrition with spineless cactus (opuntia ficus-indica) improves metabolomic profiles and pregnancy outcomes in sheep
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d93badc26da947e7a91694da19b317e8
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Periconceptional nutrition with spineless cactus (Opuntia ficus-indica) improves metabolomic profiles and pregnancy outcomes in sheep

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