Delivery of multipurpose prevention drug combinations from electrospun nanofibers using composite microarchitectures

Anna K Blakney, Emily A Krogstad, Yonghou H Jiang, Kim A WoodrowDepartment of Bioengineering, University of Washington, Seattle, Washington, USABackground: Electrospun drug-eluting fabrics have enormous potential for the delivery of physicochemically diverse drugs in combination by controlling the...

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Autores principales:	Blakney AK, Krogstad EA, Jiang YH, Woodrow KA
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/d93c881c4491446ebf8658cd39e528e1
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spelling	oai:doaj.org-article:d93c881c4491446ebf8658cd39e528e12021-12-02T02:16:44ZDelivery of multipurpose prevention drug combinations from electrospun nanofibers using composite microarchitectures1178-2013https://doaj.org/article/d93c881c4491446ebf8658cd39e528e12014-06-01T00:00:00Zhttp://www.dovepress.com/delivery-of-multipurpose-prevention-drug-combinations-from-electrospun-a17227https://doaj.org/toc/1178-2013 Anna K Blakney, Emily A Krogstad, Yonghou H Jiang, Kim A WoodrowDepartment of Bioengineering, University of Washington, Seattle, Washington, USABackground: Electrospun drug-eluting fabrics have enormous potential for the delivery of physicochemically diverse drugs in combination by controlling the underlying material chemistry and fabric microarchitecture. However, the rationale for formulating drugs at high drug loading in the same or separate fibers is unknown but has important implications for product development and clinical applications.Methods: Using a production-scale free-surface electrospinning instrument, we produced electrospun nanofibers with different microscale geometries for the co-delivery of tenofovir (TFV) and levonorgestrel (LNG) – two lead drug candidates for multipurpose prevention of HIV acquisition and unintended pregnancy. We investigated the in vitro drug release of TFV and LNG combinations from composites that deliver the two drugs from the same fiber (combined fibers) or from separate fibers in a stacked or interwoven architecture. For stacked composites, we also examined the role that fabric thickness has on drug-release kinetics. We also measured the cytotoxicity and antiviral activity of the drugs delivered alone and in combination.Results: Herein, we report on the solution and processing parameters for the free-surface electrospinning of medical fabrics with controlled microarchitecture and high drug loading (up to 20 wt%). We observed that in vitro release of the highly water-soluble TFV, but not the water-insoluble LNG, was affected by composite microarchitecture, fabric thickness, and drug content. Finally, we showed that the drug-loaded nanofibers are noncytotoxic and that the antiviral activity of TFV is preserved through the electrospinning process and when combined with LNG.Conclusion: Electrospun fabrics with high drug loading create multicomponent systems that benefit from the independent control of the nanofibrous microarchitecture. Our findings are significant because they will inform the design and production of composite electrospun fabrics for the co-delivery of physicochemically diverse drugs that may be useful for multipurpose prevention.Keywords: co-delivery, electrospinning, antiretroviral, contraceptive, microbicide, multipurpose prevention technologyBlakney AKKrogstad EAJiang YHWoodrow KADove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 2967-2978 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Blakney AK Krogstad EA Jiang YH Woodrow KA Delivery of multipurpose prevention drug combinations from electrospun nanofibers using composite microarchitectures
description	Anna K Blakney, Emily A Krogstad, Yonghou H Jiang, Kim A WoodrowDepartment of Bioengineering, University of Washington, Seattle, Washington, USABackground: Electrospun drug-eluting fabrics have enormous potential for the delivery of physicochemically diverse drugs in combination by controlling the underlying material chemistry and fabric microarchitecture. However, the rationale for formulating drugs at high drug loading in the same or separate fibers is unknown but has important implications for product development and clinical applications.Methods: Using a production-scale free-surface electrospinning instrument, we produced electrospun nanofibers with different microscale geometries for the co-delivery of tenofovir (TFV) and levonorgestrel (LNG) – two lead drug candidates for multipurpose prevention of HIV acquisition and unintended pregnancy. We investigated the in vitro drug release of TFV and LNG combinations from composites that deliver the two drugs from the same fiber (combined fibers) or from separate fibers in a stacked or interwoven architecture. For stacked composites, we also examined the role that fabric thickness has on drug-release kinetics. We also measured the cytotoxicity and antiviral activity of the drugs delivered alone and in combination.Results: Herein, we report on the solution and processing parameters for the free-surface electrospinning of medical fabrics with controlled microarchitecture and high drug loading (up to 20 wt%). We observed that in vitro release of the highly water-soluble TFV, but not the water-insoluble LNG, was affected by composite microarchitecture, fabric thickness, and drug content. Finally, we showed that the drug-loaded nanofibers are noncytotoxic and that the antiviral activity of TFV is preserved through the electrospinning process and when combined with LNG.Conclusion: Electrospun fabrics with high drug loading create multicomponent systems that benefit from the independent control of the nanofibrous microarchitecture. Our findings are significant because they will inform the design and production of composite electrospun fabrics for the co-delivery of physicochemically diverse drugs that may be useful for multipurpose prevention.Keywords: co-delivery, electrospinning, antiretroviral, contraceptive, microbicide, multipurpose prevention technology
format	article
author	Blakney AK Krogstad EA Jiang YH Woodrow KA
author_facet	Blakney AK Krogstad EA Jiang YH Woodrow KA
author_sort	Blakney AK
title	Delivery of multipurpose prevention drug combinations from electrospun nanofibers using composite microarchitectures
title_short	Delivery of multipurpose prevention drug combinations from electrospun nanofibers using composite microarchitectures
title_full	Delivery of multipurpose prevention drug combinations from electrospun nanofibers using composite microarchitectures
title_fullStr	Delivery of multipurpose prevention drug combinations from electrospun nanofibers using composite microarchitectures
title_full_unstemmed	Delivery of multipurpose prevention drug combinations from electrospun nanofibers using composite microarchitectures
title_sort	delivery of multipurpose prevention drug combinations from electrospun nanofibers using composite microarchitectures
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/d93c881c4491446ebf8658cd39e528e1
work_keys_str_mv	AT blakneyak deliveryofmultipurposepreventiondrugcombinationsfromelectrospunnanofibersusingcompositemicroarchitectures AT krogstadea deliveryofmultipurposepreventiondrugcombinationsfromelectrospunnanofibersusingcompositemicroarchitectures AT jiangyh deliveryofmultipurposepreventiondrugcombinationsfromelectrospunnanofibersusingcompositemicroarchitectures AT woodrowka deliveryofmultipurposepreventiondrugcombinationsfromelectrospunnanofibersusingcompositemicroarchitectures
_version_	1718402622893850624

Delivery of multipurpose prevention drug combinations from electrospun nanofibers using composite microarchitectures

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