Clades of Photosynthetic Bacteria Belonging to the Genus <italic toggle="yes">Rhodopseudomonas</italic> Show Marked Diversity in Light-Harvesting Antenna Complex Gene Composition and Expression

ABSTRACT Many photosynthetic bacteria have peripheral light-harvesting (LH) antenna complexes that increase the efficiency of light energy capture. The purple nonsulfur photosynthetic bacterium Rhodopseudomonas palustris produces different types of LH complexes under high light intensities (LH2 comp...

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Autores principales:	Kathryn R. Fixen, Yasuhiro Oda, Caroline S. Harwood
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2016
Materias:	transcriptomics biotechnology phototrophs Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/d93d808755f64e83a08d7b38fb352264
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spelling	oai:doaj.org-article:d93d808755f64e83a08d7b38fb3522642021-12-02T19:48:49ZClades of Photosynthetic Bacteria Belonging to the Genus <italic toggle="yes">Rhodopseudomonas</italic> Show Marked Diversity in Light-Harvesting Antenna Complex Gene Composition and Expression10.1128/mSystems.00006-152379-5077https://doaj.org/article/d93d808755f64e83a08d7b38fb3522642016-02-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.00006-15https://doaj.org/toc/2379-5077ABSTRACT Many photosynthetic bacteria have peripheral light-harvesting (LH) antenna complexes that increase the efficiency of light energy capture. The purple nonsulfur photosynthetic bacterium Rhodopseudomonas palustris produces different types of LH complexes under high light intensities (LH2 complex) and low light intensities (LH3 and LH4 complexes). There are multiple pucBA operons that encode the α and β peptides that make up these complexes. However, low-resolution structures, amino acid similarities between the complexes, and a lack of transcription analysis have made it difficult to determine the contributions of different pucBA operons to the composition and function of different LH complexes. It was also unclear how much diversity of LH complexes exists in R. palustris and affiliated strains. To address this, we undertook an integrative genomics approach using 20 sequenced strains. Gene content analysis revealed that even closely related strains have differences in their pucBA gene content. Transcriptome analyses of the strains grown under high light and low light revealed that the patterns of expression of the pucBA operons varied among strains grown under the same conditions. We also found that one set of LH2 complex proteins compensated for the lack of an LH4 complex under low light intensities but not under extremely low light intensities, indicating that there is functional redundancy between some of the LH complexes under certain light intensities. The variation observed in LH gene composition and expression in Rhodopseudomonas strains likely reflects how they have evolved to adapt to light conditions in specific soil and water microenvironments. IMPORTANCE Rhodopseudomonas palustris is a phototrophic purple nonsulfur bacterium that adapts its photosystem to allow growth at a range of light intensities. It does this by adjusting the amount and composition of peripheral light-harvesting (LH) antenna complexes that it synthesizes. Rhodopseudomonas strains are notable for containing numerous sets of light-harvesting genes. We determined the diversity of LH complexes and their transcript levels during growth under high and low light intensities in 20 sequenced genomes of strains related to the species Rhodopseudomonas palustris. The data obtained are a resource for investigators with interests as wide-ranging as the biophysics of photosynthesis, the ecology of phototrophic bacteria, and the use of photosynthetic bacteria for biotechnology applications.Kathryn R. FixenYasuhiro OdaCaroline S. HarwoodAmerican Society for MicrobiologyarticletranscriptomicsbiotechnologyphototrophsMicrobiologyQR1-502ENmSystems, Vol 1, Iss 1 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	transcriptomics biotechnology phototrophs Microbiology QR1-502
spellingShingle	transcriptomics biotechnology phototrophs Microbiology QR1-502 Kathryn R. Fixen Yasuhiro Oda Caroline S. Harwood Clades of Photosynthetic Bacteria Belonging to the Genus <italic toggle="yes">Rhodopseudomonas</italic> Show Marked Diversity in Light-Harvesting Antenna Complex Gene Composition and Expression
description	ABSTRACT Many photosynthetic bacteria have peripheral light-harvesting (LH) antenna complexes that increase the efficiency of light energy capture. The purple nonsulfur photosynthetic bacterium Rhodopseudomonas palustris produces different types of LH complexes under high light intensities (LH2 complex) and low light intensities (LH3 and LH4 complexes). There are multiple pucBA operons that encode the α and β peptides that make up these complexes. However, low-resolution structures, amino acid similarities between the complexes, and a lack of transcription analysis have made it difficult to determine the contributions of different pucBA operons to the composition and function of different LH complexes. It was also unclear how much diversity of LH complexes exists in R. palustris and affiliated strains. To address this, we undertook an integrative genomics approach using 20 sequenced strains. Gene content analysis revealed that even closely related strains have differences in their pucBA gene content. Transcriptome analyses of the strains grown under high light and low light revealed that the patterns of expression of the pucBA operons varied among strains grown under the same conditions. We also found that one set of LH2 complex proteins compensated for the lack of an LH4 complex under low light intensities but not under extremely low light intensities, indicating that there is functional redundancy between some of the LH complexes under certain light intensities. The variation observed in LH gene composition and expression in Rhodopseudomonas strains likely reflects how they have evolved to adapt to light conditions in specific soil and water microenvironments. IMPORTANCE Rhodopseudomonas palustris is a phototrophic purple nonsulfur bacterium that adapts its photosystem to allow growth at a range of light intensities. It does this by adjusting the amount and composition of peripheral light-harvesting (LH) antenna complexes that it synthesizes. Rhodopseudomonas strains are notable for containing numerous sets of light-harvesting genes. We determined the diversity of LH complexes and their transcript levels during growth under high and low light intensities in 20 sequenced genomes of strains related to the species Rhodopseudomonas palustris. The data obtained are a resource for investigators with interests as wide-ranging as the biophysics of photosynthesis, the ecology of phototrophic bacteria, and the use of photosynthetic bacteria for biotechnology applications.
format	article
author	Kathryn R. Fixen Yasuhiro Oda Caroline S. Harwood
author_facet	Kathryn R. Fixen Yasuhiro Oda Caroline S. Harwood
author_sort	Kathryn R. Fixen
title	Clades of Photosynthetic Bacteria Belonging to the Genus <italic toggle="yes">Rhodopseudomonas</italic> Show Marked Diversity in Light-Harvesting Antenna Complex Gene Composition and Expression
title_short	Clades of Photosynthetic Bacteria Belonging to the Genus <italic toggle="yes">Rhodopseudomonas</italic> Show Marked Diversity in Light-Harvesting Antenna Complex Gene Composition and Expression
title_full	Clades of Photosynthetic Bacteria Belonging to the Genus <italic toggle="yes">Rhodopseudomonas</italic> Show Marked Diversity in Light-Harvesting Antenna Complex Gene Composition and Expression
title_fullStr	Clades of Photosynthetic Bacteria Belonging to the Genus <italic toggle="yes">Rhodopseudomonas</italic> Show Marked Diversity in Light-Harvesting Antenna Complex Gene Composition and Expression
title_full_unstemmed	Clades of Photosynthetic Bacteria Belonging to the Genus <italic toggle="yes">Rhodopseudomonas</italic> Show Marked Diversity in Light-Harvesting Antenna Complex Gene Composition and Expression
title_sort	clades of photosynthetic bacteria belonging to the genus <italic toggle="yes">rhodopseudomonas</italic> show marked diversity in light-harvesting antenna complex gene composition and expression
publisher	American Society for Microbiology
publishDate	2016
url	https://doaj.org/article/d93d808755f64e83a08d7b38fb352264
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Clades of Photosynthetic Bacteria Belonging to the Genus <italic toggle="yes">Rhodopseudomonas</italic> Show Marked Diversity in Light-Harvesting Antenna Complex Gene Composition and Expression

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