Lethal Mechanisms of Nostoc-Synthesized Silver Nanoparticles Against Different Pathogenic Bacteria

Reham Samir Hamida,1 Mohamed Abdelaal Ali,2 Doaa A Goda,3 Mayasar Ibrahim Al-Zaban4 1Molecular Biology Unit, Department of Zoology, Faculty of Science, Alexandria University, Alexandria, Egypt; 2Biotechnology Unit, Department of Plant Production, College of Food and Agriculture Science, King Saud Un...

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Autores principales:	Hamida RS, Ali MA, Goda DA, Al-Zaban MI
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	biological synthesis nanoparticles pathogenic bacteria nostoc sp. bahar_m silver antibacterial activity Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/d944b4d4b213432bb83b32b3692f893d
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spelling	oai:doaj.org-article:d944b4d4b213432bb83b32b3692f893d2021-12-02T13:48:45ZLethal Mechanisms of Nostoc-Synthesized Silver Nanoparticles Against Different Pathogenic Bacteria1178-2013https://doaj.org/article/d944b4d4b213432bb83b32b3692f893d2020-12-01T00:00:00Zhttps://www.dovepress.com/lethal-mechanisms-of-nostoc-synthesized-silver-nanoparticles-against-d-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Reham Samir Hamida,1 Mohamed Abdelaal Ali,2 Doaa A Goda,3 Mayasar Ibrahim Al-Zaban4 1Molecular Biology Unit, Department of Zoology, Faculty of Science, Alexandria University, Alexandria, Egypt; 2Biotechnology Unit, Department of Plant Production, College of Food and Agriculture Science, King Saud University, Riyadh, Saudi Arabia; 3Bioprocess Development Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), City of Scientific Research and Technological Applications (SRTA-City), Alexandria, Egypt; 4Department of Biology, College of Science Princess Nourah bint Abdulrahman University, Riyadh, Saudi ArabiaCorrespondence: Mohamed Abdelaal Ali Email mali3@ksu.edu.saMayasar Ibrahim Al-Zaban Email Mialzaban@pnu.edu.saBackground: Increasing antibiotic resistance and the emergence of multidrug-resistant (MDR) pathogens have led to the need to develop new therapeutic agents to tackle microbial infections. Nano-antibiotics are a novel generation of nanomaterials with significant antimicrobial activities that target bacterial defense systems including biofilm formation, membrane permeability, and virulence activity.Purpose: In addition to AgNO3, the current study aimed to explore for first time the antibacterial potential of silver nanoparticles synthesized by Nostoc sp. Bahar_M (N-SNPs) and their killing mechanisms against Streptococcus mutans, methicillin-resistant Staphylococcus aureus, Escherichia coli, and Salmonella typhimurium.Methods: Potential mechanisms of action of both silver species against bacteria were systematically explored using agar well diffusion, enzyme (lactate dehydrogenase (LDH) and ATPase) and antioxidant (glutathione peroxidase and catalase)   assays, and morphological examinations. qRT-PCR and SDS-PAGE were employed to investigate the effect of both treatments on mfD, flu, and hly gene expression and protein patterns, respectively.Results: N-SNPs exhibited greater biocidal activity than AgNO3 against the four tested bacteria. E. coli treated with N-SNPs showed significant surges in LDH levels, imbalances in other antioxidant and enzyme activities, and marked morphological changes, including cell membrane disruption and cytoplasmic dissolution. N-SNPs caused more significant upregulation of mfD expression and downregulation of both flu and hly expression and increased protein denaturation compared with AgNO3.Conclusion: N-SNPs exhibited significant inhibitory potential against E. coli by direct interfering with bacterial cellular structures and/or enhancing oxidative stress, indicating their potential for use as an alternative antimicrobial agent. However, the potential of N-SNPs to be usable and biocompatible antibacterial drug will evaluate by their toxicity against normal cells.Keywords: biological synthesis, nanoparticles, pathogenic bacteria, Nostoc sp. Bahar_M, silver, antibacterial activityHamida RSAli MAGoda DAAl-Zaban MIDove Medical Pressarticlebiological synthesisnanoparticlespathogenic bacterianostoc sp. bahar_msilverantibacterial activityMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 10499-10517 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	biological synthesis nanoparticles pathogenic bacteria nostoc sp. bahar_m silver antibacterial activity Medicine (General) R5-920
spellingShingle	biological synthesis nanoparticles pathogenic bacteria nostoc sp. bahar_m silver antibacterial activity Medicine (General) R5-920 Hamida RS Ali MA Goda DA Al-Zaban MI Lethal Mechanisms of Nostoc-Synthesized Silver Nanoparticles Against Different Pathogenic Bacteria
description	Reham Samir Hamida,1 Mohamed Abdelaal Ali,2 Doaa A Goda,3 Mayasar Ibrahim Al-Zaban4 1Molecular Biology Unit, Department of Zoology, Faculty of Science, Alexandria University, Alexandria, Egypt; 2Biotechnology Unit, Department of Plant Production, College of Food and Agriculture Science, King Saud University, Riyadh, Saudi Arabia; 3Bioprocess Development Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), City of Scientific Research and Technological Applications (SRTA-City), Alexandria, Egypt; 4Department of Biology, College of Science Princess Nourah bint Abdulrahman University, Riyadh, Saudi ArabiaCorrespondence: Mohamed Abdelaal Ali Email mali3@ksu.edu.saMayasar Ibrahim Al-Zaban Email Mialzaban@pnu.edu.saBackground: Increasing antibiotic resistance and the emergence of multidrug-resistant (MDR) pathogens have led to the need to develop new therapeutic agents to tackle microbial infections. Nano-antibiotics are a novel generation of nanomaterials with significant antimicrobial activities that target bacterial defense systems including biofilm formation, membrane permeability, and virulence activity.Purpose: In addition to AgNO3, the current study aimed to explore for first time the antibacterial potential of silver nanoparticles synthesized by Nostoc sp. Bahar_M (N-SNPs) and their killing mechanisms against Streptococcus mutans, methicillin-resistant Staphylococcus aureus, Escherichia coli, and Salmonella typhimurium.Methods: Potential mechanisms of action of both silver species against bacteria were systematically explored using agar well diffusion, enzyme (lactate dehydrogenase (LDH) and ATPase) and antioxidant (glutathione peroxidase and catalase)   assays, and morphological examinations. qRT-PCR and SDS-PAGE were employed to investigate the effect of both treatments on mfD, flu, and hly gene expression and protein patterns, respectively.Results: N-SNPs exhibited greater biocidal activity than AgNO3 against the four tested bacteria. E. coli treated with N-SNPs showed significant surges in LDH levels, imbalances in other antioxidant and enzyme activities, and marked morphological changes, including cell membrane disruption and cytoplasmic dissolution. N-SNPs caused more significant upregulation of mfD expression and downregulation of both flu and hly expression and increased protein denaturation compared with AgNO3.Conclusion: N-SNPs exhibited significant inhibitory potential against E. coli by direct interfering with bacterial cellular structures and/or enhancing oxidative stress, indicating their potential for use as an alternative antimicrobial agent. However, the potential of N-SNPs to be usable and biocompatible antibacterial drug will evaluate by their toxicity against normal cells.Keywords: biological synthesis, nanoparticles, pathogenic bacteria, Nostoc sp. Bahar_M, silver, antibacterial activity
format	article
author	Hamida RS Ali MA Goda DA Al-Zaban MI
author_facet	Hamida RS Ali MA Goda DA Al-Zaban MI
author_sort	Hamida RS
title	Lethal Mechanisms of Nostoc-Synthesized Silver Nanoparticles Against Different Pathogenic Bacteria
title_short	Lethal Mechanisms of Nostoc-Synthesized Silver Nanoparticles Against Different Pathogenic Bacteria
title_full	Lethal Mechanisms of Nostoc-Synthesized Silver Nanoparticles Against Different Pathogenic Bacteria
title_fullStr	Lethal Mechanisms of Nostoc-Synthesized Silver Nanoparticles Against Different Pathogenic Bacteria
title_full_unstemmed	Lethal Mechanisms of Nostoc-Synthesized Silver Nanoparticles Against Different Pathogenic Bacteria
title_sort	lethal mechanisms of nostoc-synthesized silver nanoparticles against different pathogenic bacteria
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/d944b4d4b213432bb83b32b3692f893d
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Lethal Mechanisms of Nostoc-Synthesized Silver Nanoparticles Against Different Pathogenic Bacteria

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