Effects of Adper<sup>™</sup> Scotchbond<sup>™</sup> 1 XT, Clearfil<sup>™</sup> SE Bond 2 and Scotchbond<sup>™</sup> Universal in Odontoblasts

This study aimed to assess the cytotoxicity of commercially available adhesive strategies—etch-and-rinse (Adper<sup>™</sup> Scotchbond<sup>™</sup> 1 XT, 3M ESPE, St. Paul, MN, USA, SB1), self-etch (Clearfil<sup>™</sup> SE Bond 2, Kuraray Noritake Dental Inc., Toky...

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Autores principales: Miguel Cardoso, Ana Coelho, Carlos Miguel Marto, Ana Cristina Gonçalves, Anabela Paula, Ana Bela Sarmento Ribeiro, Manuel Marques Ferreira, Maria Filomena Botelho, Mafalda Laranjo, Eunice Carrilho
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Publicado: MDPI AG 2021
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spelling oai:doaj.org-article:d94776ae5bc340d3a08ed8bbb771ddf62021-11-11T18:00:53ZEffects of Adper<sup>™</sup> Scotchbond<sup>™</sup> 1 XT, Clearfil<sup>™</sup> SE Bond 2 and Scotchbond<sup>™</sup> Universal in Odontoblasts10.3390/ma142164351996-1944https://doaj.org/article/d94776ae5bc340d3a08ed8bbb771ddf62021-10-01T00:00:00Zhttps://www.mdpi.com/1996-1944/14/21/6435https://doaj.org/toc/1996-1944This study aimed to assess the cytotoxicity of commercially available adhesive strategies—etch-and-rinse (Adper<sup>™</sup> Scotchbond<sup>™</sup> 1 XT, 3M ESPE, St. Paul, MN, USA, SB1), self-etch (Clearfil<sup>™</sup> SE Bond 2, Kuraray Noritake Dental Inc., Tokyo, Japan, CSE), and universal (Scotchbond<sup>™</sup> Universal, 3M Deutschland GmbH, Neuss, Germany, SBU). MDPC-23 cells were exposed to adhesives extracts in different concentrations and exposure times. To access cell metabolic activity, viability, types of cell death, and cell cycle, the MTT assay, SRB assay, double labeling with annexin V and propidium iodide, and labeling with propidium iodide/RNAse were performed, respectively. Cultures were stained with May-Grünwald Giemsa for qualitative cytotoxicity assessment. The SB1, CSE, and SBU extracts determined a significant reduction in cell metabolism and viability. This reduction was higher for prolonged exposures, even for less concentrated extracts. CSE extracts significantly reduced the cell’s metabolic activity at higher concentrations (50% and 100%) from 2 h of exposure. After 24 and 96 h, a metabolic activity reduction was verified for all adhesives, even at lower concentrations. These changes were dependent on the adhesive, its concentration, and the incubation time. Regarding cell viability, SBU extracts were the least cytotoxic, and CSE was significantly more cytotoxic than SB1 and SBU. The adhesives determined a reduction in viable cells and an increase in apoptotic, late apoptosis/necrosis, and necrotic cells. Moreover, on cultures exposed to SB1 and CSE extracts, a decrease in the cells in S and G2/M phases and an increase in the cells in G0/G1 phase was observed. Exposure to SBU led to an increase of cells in the S phase. In general, all adhesives determined cytotoxicity. CSE extracts were the most cytotoxic and were classified as having a higher degree of reactivity, leading to more significant inhibition of cell growth and destruction of the cell’s layers.Miguel CardosoAna CoelhoCarlos Miguel MartoAna Cristina GonçalvesAnabela PaulaAna Bela Sarmento RibeiroManuel Marques FerreiraMaria Filomena BotelhoMafalda LaranjoEunice CarrilhoMDPI AGarticledental adhesivesadhesive systemscytotoxicityOdontoblastscell cultureTechnologyTElectrical engineering. Electronics. Nuclear engineeringTK1-9971Engineering (General). Civil engineering (General)TA1-2040MicroscopyQH201-278.5Descriptive and experimental mechanicsQC120-168.85ENMaterials, Vol 14, Iss 6435, p 6435 (2021)
institution DOAJ
collection DOAJ
language EN
topic dental adhesives
adhesive systems
cytotoxicity
Odontoblasts
cell culture
Technology
T
Electrical engineering. Electronics. Nuclear engineering
TK1-9971
Engineering (General). Civil engineering (General)
TA1-2040
Microscopy
QH201-278.5
Descriptive and experimental mechanics
QC120-168.85
spellingShingle dental adhesives
adhesive systems
cytotoxicity
Odontoblasts
cell culture
Technology
T
Electrical engineering. Electronics. Nuclear engineering
TK1-9971
Engineering (General). Civil engineering (General)
TA1-2040
Microscopy
QH201-278.5
Descriptive and experimental mechanics
QC120-168.85
Miguel Cardoso
Ana Coelho
Carlos Miguel Marto
Ana Cristina Gonçalves
Anabela Paula
Ana Bela Sarmento Ribeiro
Manuel Marques Ferreira
Maria Filomena Botelho
Mafalda Laranjo
Eunice Carrilho
Effects of Adper<sup>™</sup> Scotchbond<sup>™</sup> 1 XT, Clearfil<sup>™</sup> SE Bond 2 and Scotchbond<sup>™</sup> Universal in Odontoblasts
description This study aimed to assess the cytotoxicity of commercially available adhesive strategies—etch-and-rinse (Adper<sup>™</sup> Scotchbond<sup>™</sup> 1 XT, 3M ESPE, St. Paul, MN, USA, SB1), self-etch (Clearfil<sup>™</sup> SE Bond 2, Kuraray Noritake Dental Inc., Tokyo, Japan, CSE), and universal (Scotchbond<sup>™</sup> Universal, 3M Deutschland GmbH, Neuss, Germany, SBU). MDPC-23 cells were exposed to adhesives extracts in different concentrations and exposure times. To access cell metabolic activity, viability, types of cell death, and cell cycle, the MTT assay, SRB assay, double labeling with annexin V and propidium iodide, and labeling with propidium iodide/RNAse were performed, respectively. Cultures were stained with May-Grünwald Giemsa for qualitative cytotoxicity assessment. The SB1, CSE, and SBU extracts determined a significant reduction in cell metabolism and viability. This reduction was higher for prolonged exposures, even for less concentrated extracts. CSE extracts significantly reduced the cell’s metabolic activity at higher concentrations (50% and 100%) from 2 h of exposure. After 24 and 96 h, a metabolic activity reduction was verified for all adhesives, even at lower concentrations. These changes were dependent on the adhesive, its concentration, and the incubation time. Regarding cell viability, SBU extracts were the least cytotoxic, and CSE was significantly more cytotoxic than SB1 and SBU. The adhesives determined a reduction in viable cells and an increase in apoptotic, late apoptosis/necrosis, and necrotic cells. Moreover, on cultures exposed to SB1 and CSE extracts, a decrease in the cells in S and G2/M phases and an increase in the cells in G0/G1 phase was observed. Exposure to SBU led to an increase of cells in the S phase. In general, all adhesives determined cytotoxicity. CSE extracts were the most cytotoxic and were classified as having a higher degree of reactivity, leading to more significant inhibition of cell growth and destruction of the cell’s layers.
format article
author Miguel Cardoso
Ana Coelho
Carlos Miguel Marto
Ana Cristina Gonçalves
Anabela Paula
Ana Bela Sarmento Ribeiro
Manuel Marques Ferreira
Maria Filomena Botelho
Mafalda Laranjo
Eunice Carrilho
author_facet Miguel Cardoso
Ana Coelho
Carlos Miguel Marto
Ana Cristina Gonçalves
Anabela Paula
Ana Bela Sarmento Ribeiro
Manuel Marques Ferreira
Maria Filomena Botelho
Mafalda Laranjo
Eunice Carrilho
author_sort Miguel Cardoso
title Effects of Adper<sup>™</sup> Scotchbond<sup>™</sup> 1 XT, Clearfil<sup>™</sup> SE Bond 2 and Scotchbond<sup>™</sup> Universal in Odontoblasts
title_short Effects of Adper<sup>™</sup> Scotchbond<sup>™</sup> 1 XT, Clearfil<sup>™</sup> SE Bond 2 and Scotchbond<sup>™</sup> Universal in Odontoblasts
title_full Effects of Adper<sup>™</sup> Scotchbond<sup>™</sup> 1 XT, Clearfil<sup>™</sup> SE Bond 2 and Scotchbond<sup>™</sup> Universal in Odontoblasts
title_fullStr Effects of Adper<sup>™</sup> Scotchbond<sup>™</sup> 1 XT, Clearfil<sup>™</sup> SE Bond 2 and Scotchbond<sup>™</sup> Universal in Odontoblasts
title_full_unstemmed Effects of Adper<sup>™</sup> Scotchbond<sup>™</sup> 1 XT, Clearfil<sup>™</sup> SE Bond 2 and Scotchbond<sup>™</sup> Universal in Odontoblasts
title_sort effects of adper<sup>™</sup> scotchbond<sup>™</sup> 1 xt, clearfil<sup>™</sup> se bond 2 and scotchbond<sup>™</sup> universal in odontoblasts
publisher MDPI AG
publishDate 2021
url https://doaj.org/article/d94776ae5bc340d3a08ed8bbb771ddf6
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