Anti-Candidal Activity of the Parasitic Plant Orobanche crenata Forssk

Candida albicans (C. albicans) and Candida glabrata (C. glabrata) are part of the human microbiome. However, they possess numerous virulence factors, which confer them the ability to cause both local and systemic infections....

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Autores principales:	Floriana D’Angeli, Fiorella Guadagni, Carlo Genovese, Daria Nicolosi, Angela Trovato Salinaro, Mariarita Spampinato, Giuliana Mannino, Debora Lo Furno, Giulio Petronio Petronio, Simone Ronsisvalle, Federica Sipala, Luca Falzone, Vittorio Calabrese
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Orobanche crenata</i> parasitic plant <i>Candida</i> spp. phenotypic switching biofilm adhesion Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/d947c39e7678479db62e2db0080e08ff
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spelling	oai:doaj.org-article:d947c39e7678479db62e2db0080e08ff2021-11-25T16:24:09ZAnti-Candidal Activity of the Parasitic Plant <i>Orobanche crenata</i> Forssk10.3390/antibiotics101113732079-6382https://doaj.org/article/d947c39e7678479db62e2db0080e08ff2021-11-01T00:00:00Zhttps://www.mdpi.com/2079-6382/10/11/1373https://doaj.org/toc/2079-6382<i>Candida albicans</i> (<i>C. albicans</i>) and <i>Candida glabrata</i> (<i>C. glabrata</i>) are part of the human microbiome. However, they possess numerous virulence factors, which confer them the ability to cause both local and systemic infections. Candidiasis can involve multiple organs, including the eye. In the present study, we investigated the anti-candidal activity and the re-epithelizing effect of <i>Orobanche crenata</i> leaf extract (OCLE). By the microdilution method, we demonstrated an inhibitory effect of OCLE on both <i>C. albicans</i> and <i>C. glabrata</i> growth. By crystal violet and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, we showed the ability of OCLE to inhibit the biofilm formation and the viability of yeast cells, respectively. By germ tube and adhesion assays, we proved the capacity of OCLE to affect the morphological transition of <i>C. albicans</i> and the adhesion of both pathogens to human retinal pigment epithelial cells (ARPE-19), respectively. Besides, by MTT and wound healing assay, we evaluated the cytotoxic and re-epithelizing effects of OCLE on ARPE-19. Finally, the Folin–Ciocalteu and the ultra-performance liquid chromatography-tandem mass spectrometry revealed a high content of phenols and the presence of several bioactive molecules in the extract. Our results highlighted new properties of <i>O. crenata</i>, useful in the control of <i>Candida</i> infections.Floriana D’AngeliFiorella GuadagniCarlo GenoveseDaria NicolosiAngela Trovato SalinaroMariarita SpampinatoGiuliana ManninoDebora Lo FurnoGiulio Petronio PetronioSimone RonsisvalleFederica SipalaLuca FalzoneVittorio CalabreseMDPI AGarticle<i>Orobanche crenata</i>parasitic plant<i>Candida</i> spp.phenotypic switchingbiofilmadhesionTherapeutics. PharmacologyRM1-950ENAntibiotics, Vol 10, Iss 1373, p 1373 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Orobanche crenata</i> parasitic plant <i>Candida</i> spp. phenotypic switching biofilm adhesion Therapeutics. Pharmacology RM1-950
spellingShingle	<i>Orobanche crenata</i> parasitic plant <i>Candida</i> spp. phenotypic switching biofilm adhesion Therapeutics. Pharmacology RM1-950 Floriana D’Angeli Fiorella Guadagni Carlo Genovese Daria Nicolosi Angela Trovato Salinaro Mariarita Spampinato Giuliana Mannino Debora Lo Furno Giulio Petronio Petronio Simone Ronsisvalle Federica Sipala Luca Falzone Vittorio Calabrese Anti-Candidal Activity of the Parasitic Plant <i>Orobanche crenata</i> Forssk
description	<i>Candida albicans</i> (<i>C. albicans</i>) and <i>Candida glabrata</i> (<i>C. glabrata</i>) are part of the human microbiome. However, they possess numerous virulence factors, which confer them the ability to cause both local and systemic infections. Candidiasis can involve multiple organs, including the eye. In the present study, we investigated the anti-candidal activity and the re-epithelizing effect of <i>Orobanche crenata</i> leaf extract (OCLE). By the microdilution method, we demonstrated an inhibitory effect of OCLE on both <i>C. albicans</i> and <i>C. glabrata</i> growth. By crystal violet and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, we showed the ability of OCLE to inhibit the biofilm formation and the viability of yeast cells, respectively. By germ tube and adhesion assays, we proved the capacity of OCLE to affect the morphological transition of <i>C. albicans</i> and the adhesion of both pathogens to human retinal pigment epithelial cells (ARPE-19), respectively. Besides, by MTT and wound healing assay, we evaluated the cytotoxic and re-epithelizing effects of OCLE on ARPE-19. Finally, the Folin–Ciocalteu and the ultra-performance liquid chromatography-tandem mass spectrometry revealed a high content of phenols and the presence of several bioactive molecules in the extract. Our results highlighted new properties of <i>O. crenata</i>, useful in the control of <i>Candida</i> infections.
format	article
author	Floriana D’Angeli Fiorella Guadagni Carlo Genovese Daria Nicolosi Angela Trovato Salinaro Mariarita Spampinato Giuliana Mannino Debora Lo Furno Giulio Petronio Petronio Simone Ronsisvalle Federica Sipala Luca Falzone Vittorio Calabrese
author_facet	Floriana D’Angeli Fiorella Guadagni Carlo Genovese Daria Nicolosi Angela Trovato Salinaro Mariarita Spampinato Giuliana Mannino Debora Lo Furno Giulio Petronio Petronio Simone Ronsisvalle Federica Sipala Luca Falzone Vittorio Calabrese
author_sort	Floriana D’Angeli
title	Anti-Candidal Activity of the Parasitic Plant <i>Orobanche crenata</i> Forssk
title_short	Anti-Candidal Activity of the Parasitic Plant <i>Orobanche crenata</i> Forssk
title_full	Anti-Candidal Activity of the Parasitic Plant <i>Orobanche crenata</i> Forssk
title_fullStr	Anti-Candidal Activity of the Parasitic Plant <i>Orobanche crenata</i> Forssk
title_full_unstemmed	Anti-Candidal Activity of the Parasitic Plant <i>Orobanche crenata</i> Forssk
title_sort	anti-candidal activity of the parasitic plant <i>orobanche crenata</i> forssk
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/d947c39e7678479db62e2db0080e08ff
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Anti-Candidal Activity of the Parasitic Plant <i>Orobanche crenata</i> Forssk

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