A pilot study of 3D tissue-engineered bone marrow culture as a tool to predict patient response to therapy in multiple myeloma

Abstract Cancer patients undergo detrimental toxicities and ineffective treatments especially in the relapsed setting, due to failed treatment attempts. The development of a tool that predicts the clinical response of individual patients to therapy is greatly desired. We have developed a novel patie...

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Autores principales:	Kinan Alhallak, Amanda Jeske, Pilar de la Puente, Jennifer Sun, Mark Fiala, Feda Azab, Barbara Muz, Ilyas Sahin, Ravi Vij, John F. DiPersio, Abdel Kareem Azab
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d949d57f50c147fba308ccbe7e50a877
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spelling	oai:doaj.org-article:d949d57f50c147fba308ccbe7e50a8772021-12-02T17:17:38ZA pilot study of 3D tissue-engineered bone marrow culture as a tool to predict patient response to therapy in multiple myeloma10.1038/s41598-021-98760-92045-2322https://doaj.org/article/d949d57f50c147fba308ccbe7e50a8772021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98760-9https://doaj.org/toc/2045-2322Abstract Cancer patients undergo detrimental toxicities and ineffective treatments especially in the relapsed setting, due to failed treatment attempts. The development of a tool that predicts the clinical response of individual patients to therapy is greatly desired. We have developed a novel patient-derived 3D tissue engineered bone marrow (3DTEBM) technology that closely recapitulate the pathophysiological conditions in the bone marrow and allows ex vivo proliferation of tumor cells of hematologic malignancies. In this study, we used the 3DTEBM to predict the clinical response of individual multiple myeloma (MM) patients to different therapeutic regimens. We found that while no correlation was observed between in vitro efficacy in classic 2D culture systems of drugs used for MM with their clinical efficacious concentration, the efficacious concentration in the 3DTEBM were directly correlated. Furthermore, the 3DTEBM model retrospectively predicted the clinical response to different treatment regimens in 89% of the MM patient cohort. These results demonstrated that the 3DTEBM is a feasible platform which can predict MM clinical responses with high accuracy and within a clinically actionable time frame. Utilization of this technology to predict drug efficacy and the likelihood of treatment failure could significantly improve patient care and treatment in many ways, particularly in the relapsed and refractory setting. Future studies are needed to validate the 3DTEBM model as a tool for predicting clinical efficacy.Kinan AlhallakAmanda JeskePilar de la PuenteJennifer SunMark FialaFeda AzabBarbara MuzIlyas SahinRavi VijJohn F. DiPersioAbdel Kareem AzabNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kinan Alhallak Amanda Jeske Pilar de la Puente Jennifer Sun Mark Fiala Feda Azab Barbara Muz Ilyas Sahin Ravi Vij John F. DiPersio Abdel Kareem Azab A pilot study of 3D tissue-engineered bone marrow culture as a tool to predict patient response to therapy in multiple myeloma
description	Abstract Cancer patients undergo detrimental toxicities and ineffective treatments especially in the relapsed setting, due to failed treatment attempts. The development of a tool that predicts the clinical response of individual patients to therapy is greatly desired. We have developed a novel patient-derived 3D tissue engineered bone marrow (3DTEBM) technology that closely recapitulate the pathophysiological conditions in the bone marrow and allows ex vivo proliferation of tumor cells of hematologic malignancies. In this study, we used the 3DTEBM to predict the clinical response of individual multiple myeloma (MM) patients to different therapeutic regimens. We found that while no correlation was observed between in vitro efficacy in classic 2D culture systems of drugs used for MM with their clinical efficacious concentration, the efficacious concentration in the 3DTEBM were directly correlated. Furthermore, the 3DTEBM model retrospectively predicted the clinical response to different treatment regimens in 89% of the MM patient cohort. These results demonstrated that the 3DTEBM is a feasible platform which can predict MM clinical responses with high accuracy and within a clinically actionable time frame. Utilization of this technology to predict drug efficacy and the likelihood of treatment failure could significantly improve patient care and treatment in many ways, particularly in the relapsed and refractory setting. Future studies are needed to validate the 3DTEBM model as a tool for predicting clinical efficacy.
format	article
author	Kinan Alhallak Amanda Jeske Pilar de la Puente Jennifer Sun Mark Fiala Feda Azab Barbara Muz Ilyas Sahin Ravi Vij John F. DiPersio Abdel Kareem Azab
author_facet	Kinan Alhallak Amanda Jeske Pilar de la Puente Jennifer Sun Mark Fiala Feda Azab Barbara Muz Ilyas Sahin Ravi Vij John F. DiPersio Abdel Kareem Azab
author_sort	Kinan Alhallak
title	A pilot study of 3D tissue-engineered bone marrow culture as a tool to predict patient response to therapy in multiple myeloma
title_short	A pilot study of 3D tissue-engineered bone marrow culture as a tool to predict patient response to therapy in multiple myeloma
title_full	A pilot study of 3D tissue-engineered bone marrow culture as a tool to predict patient response to therapy in multiple myeloma
title_fullStr	A pilot study of 3D tissue-engineered bone marrow culture as a tool to predict patient response to therapy in multiple myeloma
title_full_unstemmed	A pilot study of 3D tissue-engineered bone marrow culture as a tool to predict patient response to therapy in multiple myeloma
title_sort	pilot study of 3d tissue-engineered bone marrow culture as a tool to predict patient response to therapy in multiple myeloma
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d949d57f50c147fba308ccbe7e50a877
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A pilot study of 3D tissue-engineered bone marrow culture as a tool to predict patient response to therapy in multiple myeloma

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