Biodiversity and phylogenetic analyses using DNA barcoding rbcL gene of seagrass from Sekotong, West Lombok, Indonesia

Abstract. Stevanus, Pharmawati M. 2021. Biodiversity and phylogenetic analyses using DNA barcoding rbcL gene of seagrass from Sekotong, West Lombok, Indonesia. Biodiversitas 22: 50-57. West Lombok, Indonesia is one of the locations that is thought to have a quite high diversity of seagrass. Informat...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Stevanus STEVANUS, Made Pharmawati
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	biodiversity, phylogenetic, rbcl gene, seagrass, west lombok Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/d94cd3e22eb04f4e8596000ec689de8d
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d94cd3e22eb04f4e8596000ec689de8d
record_format	dspace
spelling	oai:doaj.org-article:d94cd3e22eb04f4e8596000ec689de8d2021-11-22T00:53:57ZBiodiversity and phylogenetic analyses using DNA barcoding rbcL gene of seagrass from Sekotong, West Lombok, Indonesia1412-033X2085-472210.13057/biodiv/d220107https://doaj.org/article/d94cd3e22eb04f4e8596000ec689de8d2020-12-01T00:00:00Zhttps://smujo.id/biodiv/article/view/7027https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Stevanus, Pharmawati M. 2021. Biodiversity and phylogenetic analyses using DNA barcoding rbcL gene of seagrass from Sekotong, West Lombok, Indonesia. Biodiversitas 22: 50-57. West Lombok, Indonesia is one of the locations that is thought to have a quite high diversity of seagrass. Information on the diversity of seagrass species is important due to the important value of seagrass in the marine ecosystem. This research aimed to analyze biodiversity and phylogenetic of seagrass from Sekotong, West Lombok, Indonesia using DNA barcoding of rbcL gene. As many as 35 samples from seven morphologically identified species (Enhalus acoroides, Thalassia hemprichii, Cymodocea rotundata, Syringodium isoetifolium, Halodule pinifolia, Halophila ovalis, and H. minor) were taken from four Gilis (small island) in Sekotong. The DNA was amplified for the rbcL gene and sequence analyses using BLAST were conducted to determine the species. Phylogenetic analyses were carried out using three evolutionary algorithms using Neighbor-Joining, Maximum Likelihood and Bayesian analysis with 1000 bootstrap. The rbcL gene was successfully amplified from all samples with a maximum length of 552 bp. The phylogenetic analysis showed that clades were split by family and genera where six clades were formed (Enhalus acoroides, T. hemprichii, Halophila complex, H. pinifolia, S. isoetifolium, and C. rotundata) with more than 95% of bootstrap values for Neighbor-Joining and Bayesian. The p-distance values between species were 0.008-0.097 and the polymorphic site was not found within species. The rbcL sequences only confirmed five seagrass species out of seven morphologically identified species and the sequences generated from this study cannot discriminate Halophila ovalis and H. minor.Stevanus STEVANUSMade PharmawatiMBI & UNS Soloarticlebiodiversity, phylogenetic, rbcl gene, seagrass, west lombokBiology (General)QH301-705.5ENBiodiversitas, Vol 22, Iss 1 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	biodiversity, phylogenetic, rbcl gene, seagrass, west lombok Biology (General) QH301-705.5
spellingShingle	biodiversity, phylogenetic, rbcl gene, seagrass, west lombok Biology (General) QH301-705.5 Stevanus STEVANUS Made Pharmawati Biodiversity and phylogenetic analyses using DNA barcoding rbcL gene of seagrass from Sekotong, West Lombok, Indonesia
description	Abstract. Stevanus, Pharmawati M. 2021. Biodiversity and phylogenetic analyses using DNA barcoding rbcL gene of seagrass from Sekotong, West Lombok, Indonesia. Biodiversitas 22: 50-57. West Lombok, Indonesia is one of the locations that is thought to have a quite high diversity of seagrass. Information on the diversity of seagrass species is important due to the important value of seagrass in the marine ecosystem. This research aimed to analyze biodiversity and phylogenetic of seagrass from Sekotong, West Lombok, Indonesia using DNA barcoding of rbcL gene. As many as 35 samples from seven morphologically identified species (Enhalus acoroides, Thalassia hemprichii, Cymodocea rotundata, Syringodium isoetifolium, Halodule pinifolia, Halophila ovalis, and H. minor) were taken from four Gilis (small island) in Sekotong. The DNA was amplified for the rbcL gene and sequence analyses using BLAST were conducted to determine the species. Phylogenetic analyses were carried out using three evolutionary algorithms using Neighbor-Joining, Maximum Likelihood and Bayesian analysis with 1000 bootstrap. The rbcL gene was successfully amplified from all samples with a maximum length of 552 bp. The phylogenetic analysis showed that clades were split by family and genera where six clades were formed (Enhalus acoroides, T. hemprichii, Halophila complex, H. pinifolia, S. isoetifolium, and C. rotundata) with more than 95% of bootstrap values for Neighbor-Joining and Bayesian. The p-distance values between species were 0.008-0.097 and the polymorphic site was not found within species. The rbcL sequences only confirmed five seagrass species out of seven morphologically identified species and the sequences generated from this study cannot discriminate Halophila ovalis and H. minor.
format	article
author	Stevanus STEVANUS Made Pharmawati
author_facet	Stevanus STEVANUS Made Pharmawati
author_sort	Stevanus STEVANUS
title	Biodiversity and phylogenetic analyses using DNA barcoding rbcL gene of seagrass from Sekotong, West Lombok, Indonesia
title_short	Biodiversity and phylogenetic analyses using DNA barcoding rbcL gene of seagrass from Sekotong, West Lombok, Indonesia
title_full	Biodiversity and phylogenetic analyses using DNA barcoding rbcL gene of seagrass from Sekotong, West Lombok, Indonesia
title_fullStr	Biodiversity and phylogenetic analyses using DNA barcoding rbcL gene of seagrass from Sekotong, West Lombok, Indonesia
title_full_unstemmed	Biodiversity and phylogenetic analyses using DNA barcoding rbcL gene of seagrass from Sekotong, West Lombok, Indonesia
title_sort	biodiversity and phylogenetic analyses using dna barcoding rbcl gene of seagrass from sekotong, west lombok, indonesia
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/d94cd3e22eb04f4e8596000ec689de8d
work_keys_str_mv	AT stevanusstevanus biodiversityandphylogeneticanalysesusingdnabarcodingrbclgeneofseagrassfromsekotongwestlombokindonesia AT madepharmawati biodiversityandphylogeneticanalysesusingdnabarcodingrbclgeneofseagrassfromsekotongwestlombokindonesia
_version_	1718418492188786688

Biodiversity and phylogenetic analyses using DNA barcoding rbcL gene of seagrass from Sekotong, West Lombok, Indonesia

Ejemplares similares