The gene expression profiles in response to 102 traditional Chinese medicine (TCM) components: a general template for research on TCMs

Abstract Traditional Chinese medicines (TCMs) have important therapeutic value in long-term clinical practice. However, because TCMs contain diverse ingredients and have complex effects on the human body, the molecular mechanisms of TCMs are poorly understood. In this work, we determined the gene ex...

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Autores principales:	Chao Lv, Xueting Wu, Xia Wang, Juan Su, Huawu Zeng, Jing Zhao, Shan Lin, Runhui Liu, Honglin Li, Xuan Li, Weidong Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d9503e10506e4c0385f4f2ea65efc3da
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spelling	oai:doaj.org-article:d9503e10506e4c0385f4f2ea65efc3da2021-12-02T15:05:31ZThe gene expression profiles in response to 102 traditional Chinese medicine (TCM) components: a general template for research on TCMs10.1038/s41598-017-00535-82045-2322https://doaj.org/article/d9503e10506e4c0385f4f2ea65efc3da2017-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00535-8https://doaj.org/toc/2045-2322Abstract Traditional Chinese medicines (TCMs) have important therapeutic value in long-term clinical practice. However, because TCMs contain diverse ingredients and have complex effects on the human body, the molecular mechanisms of TCMs are poorly understood. In this work, we determined the gene expression profiles of cells in response to TCM components to investigate TCM activities at the molecular and cellular levels. MCF7 cells were separately treated with 102 different molecules from TCMs, and their gene expression profiles were compared with the Connectivity Map (CMAP). To demonstrate the reliability and utility of our approach, we used nitidine chloride (NC) from the root of Zanthoxylum nitidum, a topoisomerase I/II inhibitor and α-adrenoreceptor antagonist, as an example to study the molecular function of TCMs using CMAP data as references. We successfully applied this approach to the four ingredients in Danshen and analyzed the synergistic mechanism of TCM components. The results demonstrate that our newly generated TCM data and related methods are valuable in the analysis and discovery of the molecular actions of TCM components. This is the first work to establish gene expression profiles for the study of TCM components and serves as a template for general TCM research.Chao LvXueting WuXia WangJuan SuHuawu ZengJing ZhaoShan LinRunhui LiuHonglin LiXuan LiWeidong ZhangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chao Lv Xueting Wu Xia Wang Juan Su Huawu Zeng Jing Zhao Shan Lin Runhui Liu Honglin Li Xuan Li Weidong Zhang The gene expression profiles in response to 102 traditional Chinese medicine (TCM) components: a general template for research on TCMs
description	Abstract Traditional Chinese medicines (TCMs) have important therapeutic value in long-term clinical practice. However, because TCMs contain diverse ingredients and have complex effects on the human body, the molecular mechanisms of TCMs are poorly understood. In this work, we determined the gene expression profiles of cells in response to TCM components to investigate TCM activities at the molecular and cellular levels. MCF7 cells were separately treated with 102 different molecules from TCMs, and their gene expression profiles were compared with the Connectivity Map (CMAP). To demonstrate the reliability and utility of our approach, we used nitidine chloride (NC) from the root of Zanthoxylum nitidum, a topoisomerase I/II inhibitor and α-adrenoreceptor antagonist, as an example to study the molecular function of TCMs using CMAP data as references. We successfully applied this approach to the four ingredients in Danshen and analyzed the synergistic mechanism of TCM components. The results demonstrate that our newly generated TCM data and related methods are valuable in the analysis and discovery of the molecular actions of TCM components. This is the first work to establish gene expression profiles for the study of TCM components and serves as a template for general TCM research.
format	article
author	Chao Lv Xueting Wu Xia Wang Juan Su Huawu Zeng Jing Zhao Shan Lin Runhui Liu Honglin Li Xuan Li Weidong Zhang
author_facet	Chao Lv Xueting Wu Xia Wang Juan Su Huawu Zeng Jing Zhao Shan Lin Runhui Liu Honglin Li Xuan Li Weidong Zhang
author_sort	Chao Lv
title	The gene expression profiles in response to 102 traditional Chinese medicine (TCM) components: a general template for research on TCMs
title_short	The gene expression profiles in response to 102 traditional Chinese medicine (TCM) components: a general template for research on TCMs
title_full	The gene expression profiles in response to 102 traditional Chinese medicine (TCM) components: a general template for research on TCMs
title_fullStr	The gene expression profiles in response to 102 traditional Chinese medicine (TCM) components: a general template for research on TCMs
title_full_unstemmed	The gene expression profiles in response to 102 traditional Chinese medicine (TCM) components: a general template for research on TCMs
title_sort	gene expression profiles in response to 102 traditional chinese medicine (tcm) components: a general template for research on tcms
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/d9503e10506e4c0385f4f2ea65efc3da
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The gene expression profiles in response to 102 traditional Chinese medicine (TCM) components: a general template for research on TCMs

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