Cancer stem cell-like side population cells in clear cell renal cell carcinoma cell line 769P.

Although cancers are widely considered to be maintained by stem cells, the existence of stem cells in renal cell carcinoma (RCC) has seldom been reported, in part due to the lack of unique surface markers. We here identified cancer stem cell-like cells with side population (SP) phenotype in five hum...

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Autores principales:	Bin Huang, Yi Jun Huang, Zhi Jun Yao, Xu Chen, Sheng Jie Guo, Xiao Peng Mao, Dao Hu Wang, Jun Xing Chen, Shao Peng Qiu
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d95281571ee2485f987d81e062d996a0
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spelling	oai:doaj.org-article:d95281571ee2485f987d81e062d996a02021-11-18T07:37:50ZCancer stem cell-like side population cells in clear cell renal cell carcinoma cell line 769P.1932-620310.1371/journal.pone.0068293https://doaj.org/article/d95281571ee2485f987d81e062d996a02013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23874578/?tool=EBIhttps://doaj.org/toc/1932-6203Although cancers are widely considered to be maintained by stem cells, the existence of stem cells in renal cell carcinoma (RCC) has seldom been reported, in part due to the lack of unique surface markers. We here identified cancer stem cell-like cells with side population (SP) phenotype in five human RCC cell lines. Flow cytometry analysis revealed that 769P, a human clear cell RCC cell line, contained the largest amount of SP cells as compared with other four cell lines. These 769P SP cells possessed characteristics of proliferation, self-renewal, and differentiation, as well as strong resistance to chemotherapy and radiotherapy that were possibly related to the ABCB1 transporter. In vivo experiments with serial tumor transplantation in mice also showed that 769P SP cells formed tumors in NOD/SCID mice. Taken together, these results indicate that 769P SP cells have the properties of cancer stem cells, which may play important roles in tumorigenesis and therapy-resistance of RCC.Bin HuangYi Jun HuangZhi Jun YaoXu ChenSheng Jie GuoXiao Peng MaoDao Hu WangJun Xing ChenShao Peng QiuPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 7, p e68293 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Bin Huang Yi Jun Huang Zhi Jun Yao Xu Chen Sheng Jie Guo Xiao Peng Mao Dao Hu Wang Jun Xing Chen Shao Peng Qiu Cancer stem cell-like side population cells in clear cell renal cell carcinoma cell line 769P.
description	Although cancers are widely considered to be maintained by stem cells, the existence of stem cells in renal cell carcinoma (RCC) has seldom been reported, in part due to the lack of unique surface markers. We here identified cancer stem cell-like cells with side population (SP) phenotype in five human RCC cell lines. Flow cytometry analysis revealed that 769P, a human clear cell RCC cell line, contained the largest amount of SP cells as compared with other four cell lines. These 769P SP cells possessed characteristics of proliferation, self-renewal, and differentiation, as well as strong resistance to chemotherapy and radiotherapy that were possibly related to the ABCB1 transporter. In vivo experiments with serial tumor transplantation in mice also showed that 769P SP cells formed tumors in NOD/SCID mice. Taken together, these results indicate that 769P SP cells have the properties of cancer stem cells, which may play important roles in tumorigenesis and therapy-resistance of RCC.
format	article
author	Bin Huang Yi Jun Huang Zhi Jun Yao Xu Chen Sheng Jie Guo Xiao Peng Mao Dao Hu Wang Jun Xing Chen Shao Peng Qiu
author_facet	Bin Huang Yi Jun Huang Zhi Jun Yao Xu Chen Sheng Jie Guo Xiao Peng Mao Dao Hu Wang Jun Xing Chen Shao Peng Qiu
author_sort	Bin Huang
title	Cancer stem cell-like side population cells in clear cell renal cell carcinoma cell line 769P.
title_short	Cancer stem cell-like side population cells in clear cell renal cell carcinoma cell line 769P.
title_full	Cancer stem cell-like side population cells in clear cell renal cell carcinoma cell line 769P.
title_fullStr	Cancer stem cell-like side population cells in clear cell renal cell carcinoma cell line 769P.
title_full_unstemmed	Cancer stem cell-like side population cells in clear cell renal cell carcinoma cell line 769P.
title_sort	cancer stem cell-like side population cells in clear cell renal cell carcinoma cell line 769p.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/d95281571ee2485f987d81e062d996a0
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Cancer stem cell-like side population cells in clear cell renal cell carcinoma cell line 769P.

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