Quercetin-treated rat sperm enables refrigerated transport with motility and fertility for five days

Abstract Shipment of laboratory rats between animal facilities is frequently performed using special containers. However, the shipment of live animals is associated with potential risks of infectious diseases, escape and death during shipment and animal welfare issues. The transport of cold-stored s...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Katsuma Yamaga, Satohiro Nakao, Nobuyuki Mikoda, Hidetaka Yoshimoto, Ena Nakatsukasa, Naomi Nakagata, Toru Takeo
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d9562981f159469689a0ae9bc2299f33
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d9562981f159469689a0ae9bc2299f33
record_format	dspace
spelling	oai:doaj.org-article:d9562981f159469689a0ae9bc2299f332021-11-28T12:15:49ZQuercetin-treated rat sperm enables refrigerated transport with motility and fertility for five days10.1038/s41598-021-02166-62045-2322https://doaj.org/article/d9562981f159469689a0ae9bc2299f332021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-02166-6https://doaj.org/toc/2045-2322Abstract Shipment of laboratory rats between animal facilities is frequently performed using special containers. However, the shipment of live animals is associated with potential risks of infectious diseases, escape and death during shipment and animal welfare issues. The transport of cold-stored sperm avoids such risks; however, there have been no reports on cold storage of rat sperm. We previously reported that dimethyl sulfoxide (DMSO) and quercetin maintained the motility and fertilising abilities of cold-stored mouse sperm stored for 10 days. The present study investigated the efficacy of DMSO and quercetin in the cold storage of rat sperm. Quercetin maintained motility and fertility of cold-stored rat sperm stored for 5 days. After in vitro fertilisation using cold-stored sperm, pronuclear and two-cell embryos developed normally to pups following embryo transfer. Therefore, we demonstrated that live pups could be obtained from sperm transported using the cold-storage system. We conclude that cold storage of rat sperm may provide an efficient system for transporting rat resources as an alternative to shipping live animals.Katsuma YamagaSatohiro NakaoNobuyuki MikodaHidetaka YoshimotoEna NakatsukasaNaomi NakagataToru TakeoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Katsuma Yamaga Satohiro Nakao Nobuyuki Mikoda Hidetaka Yoshimoto Ena Nakatsukasa Naomi Nakagata Toru Takeo Quercetin-treated rat sperm enables refrigerated transport with motility and fertility for five days
description	Abstract Shipment of laboratory rats between animal facilities is frequently performed using special containers. However, the shipment of live animals is associated with potential risks of infectious diseases, escape and death during shipment and animal welfare issues. The transport of cold-stored sperm avoids such risks; however, there have been no reports on cold storage of rat sperm. We previously reported that dimethyl sulfoxide (DMSO) and quercetin maintained the motility and fertilising abilities of cold-stored mouse sperm stored for 10 days. The present study investigated the efficacy of DMSO and quercetin in the cold storage of rat sperm. Quercetin maintained motility and fertility of cold-stored rat sperm stored for 5 days. After in vitro fertilisation using cold-stored sperm, pronuclear and two-cell embryos developed normally to pups following embryo transfer. Therefore, we demonstrated that live pups could be obtained from sperm transported using the cold-storage system. We conclude that cold storage of rat sperm may provide an efficient system for transporting rat resources as an alternative to shipping live animals.
format	article
author	Katsuma Yamaga Satohiro Nakao Nobuyuki Mikoda Hidetaka Yoshimoto Ena Nakatsukasa Naomi Nakagata Toru Takeo
author_facet	Katsuma Yamaga Satohiro Nakao Nobuyuki Mikoda Hidetaka Yoshimoto Ena Nakatsukasa Naomi Nakagata Toru Takeo
author_sort	Katsuma Yamaga
title	Quercetin-treated rat sperm enables refrigerated transport with motility and fertility for five days
title_short	Quercetin-treated rat sperm enables refrigerated transport with motility and fertility for five days
title_full	Quercetin-treated rat sperm enables refrigerated transport with motility and fertility for five days
title_fullStr	Quercetin-treated rat sperm enables refrigerated transport with motility and fertility for five days
title_full_unstemmed	Quercetin-treated rat sperm enables refrigerated transport with motility and fertility for five days
title_sort	quercetin-treated rat sperm enables refrigerated transport with motility and fertility for five days
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d9562981f159469689a0ae9bc2299f33
work_keys_str_mv	AT katsumayamaga quercetintreatedratspermenablesrefrigeratedtransportwithmotilityandfertilityforfivedays AT satohironakao quercetintreatedratspermenablesrefrigeratedtransportwithmotilityandfertilityforfivedays AT nobuyukimikoda quercetintreatedratspermenablesrefrigeratedtransportwithmotilityandfertilityforfivedays AT hidetakayoshimoto quercetintreatedratspermenablesrefrigeratedtransportwithmotilityandfertilityforfivedays AT enanakatsukasa quercetintreatedratspermenablesrefrigeratedtransportwithmotilityandfertilityforfivedays AT naominakagata quercetintreatedratspermenablesrefrigeratedtransportwithmotilityandfertilityforfivedays AT torutakeo quercetintreatedratspermenablesrefrigeratedtransportwithmotilityandfertilityforfivedays
_version_	1718408106002612224

Quercetin-treated rat sperm enables refrigerated transport with motility and fertility for five days

Ejemplares similares