Neuroprotective Potential of Tamarillo (Cyphomandra betacea) Epicarp Extracts Obtained by Sustainable Extraction Process

Tamarillo (Cyphomandra betacea (Cav.) Sendt.), or tree tomato, is a tropical fruit from the Andean region of South America; it is highly rich in vitamins, minerals, and polyphenolic compounds. In this study, extracts from tamarillo epicarp (TE) were obtained by pressurized liquid extraction (PLE), a...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zully Jimena Suárez-Montenegro, Diego Ballesteros-Vivas, Rocío Gallego, Alberto Valdés, Jose David Sánchez-Martínez, Fabián Parada-Alfonso, Elena Ibáñez, Alejandro Cifuentes
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	neuroprotective activity Alzheimer's disease Cyphomandra betacea tropical fruit by-product green extraction pressurized liquid extraction Nutrition. Foods and food supply TX341-641
Acceso en línea:	https://doaj.org/article/d9573b0a87d84d9bacfc049626534c7c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d9573b0a87d84d9bacfc049626534c7c
record_format	dspace
spelling	oai:doaj.org-article:d9573b0a87d84d9bacfc049626534c7c2021-11-15T06:57:27ZNeuroprotective Potential of Tamarillo (Cyphomandra betacea) Epicarp Extracts Obtained by Sustainable Extraction Process2296-861X10.3389/fnut.2021.769617https://doaj.org/article/d9573b0a87d84d9bacfc049626534c7c2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fnut.2021.769617/fullhttps://doaj.org/toc/2296-861XTamarillo (Cyphomandra betacea (Cav.) Sendt.), or tree tomato, is a tropical fruit from the Andean region of South America; it is highly rich in vitamins, minerals, and polyphenolic compounds. In this study, extracts from tamarillo epicarp (TE) were obtained by pressurized liquid extraction (PLE), and their in-vitro neuroprotective potential was assessed. A central composite design with response surface methodology was performed to optimize PLE as a function of solvent composition and temperature. Selected response variables were extraction yield, total phenolic content (TPC), total flavonoid content (TFC), total carotenoid content (TCC), antioxidant (ABTS), and anti-inflammatory (LOX) activities, and anti-acetylcholinesterase (AChE) inhibitory capacity. According to the desirability function, the optimal conditions were 100% ethanol and 180°C with a 0.87 desirability value. Next, the anti-butyrylcholinesterase enzyme (BChE), reactive oxygen species (ROS), and reactive nitrogen species (RNS) inhibition as well as cytotoxicity in HK-2, THP-1 monocytes, and SH-5YSY neuroblastoma cell lines were studied for the TE extract obtained under optimized conditions. The optimum TE extract provided the following results: extraction yield (36.25%), TPC (92.09 mg GAE/g extract), TFC (4.4 mg QE/g extract), TCC (107.15 mg CE/g extract), antioxidant capacity (ABTS, IC50 = 6.33 mg/ml extract), LOX (IC50 = 48.3 mg/ml extract), and AChE (IC50 = 97.46 mg/ml extract), and showed no toxicity at concentration up to 120 μg/ml extract for all the tested cell lines. Finally, chemical characterization by liquid chromatography-tandem mass spectrometry (UHPLC-q-TOF-MS/MS) of the optimum TE extract exhibited an important presence of hydroxycinnamic acid derivatives and other phenolic acids as well as quercetin hexoside and rutin, as main metabolites responsible for the observed biological properties. All these results suggested that TE, which represents between 8 and 15% of the total fruit, could become a promising natural by-product with a potential “multitarget” activity against Alzheimer's disease.Zully Jimena Suárez-MontenegroZully Jimena Suárez-MontenegroDiego Ballesteros-VivasDiego Ballesteros-VivasRocío GallegoAlberto ValdésJose David Sánchez-MartínezFabián Parada-AlfonsoElena IbáñezAlejandro CifuentesFrontiers Media S.A.articleneuroprotective activityAlzheimer's diseaseCyphomandra betaceatropical fruit by-productgreen extractionpressurized liquid extractionNutrition. Foods and food supplyTX341-641ENFrontiers in Nutrition, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	neuroprotective activity Alzheimer's disease Cyphomandra betacea tropical fruit by-product green extraction pressurized liquid extraction Nutrition. Foods and food supply TX341-641
spellingShingle	neuroprotective activity Alzheimer's disease Cyphomandra betacea tropical fruit by-product green extraction pressurized liquid extraction Nutrition. Foods and food supply TX341-641 Zully Jimena Suárez-Montenegro Zully Jimena Suárez-Montenegro Diego Ballesteros-Vivas Diego Ballesteros-Vivas Rocío Gallego Alberto Valdés Jose David Sánchez-Martínez Fabián Parada-Alfonso Elena Ibáñez Alejandro Cifuentes Neuroprotective Potential of Tamarillo (Cyphomandra betacea) Epicarp Extracts Obtained by Sustainable Extraction Process
description	Tamarillo (Cyphomandra betacea (Cav.) Sendt.), or tree tomato, is a tropical fruit from the Andean region of South America; it is highly rich in vitamins, minerals, and polyphenolic compounds. In this study, extracts from tamarillo epicarp (TE) were obtained by pressurized liquid extraction (PLE), and their in-vitro neuroprotective potential was assessed. A central composite design with response surface methodology was performed to optimize PLE as a function of solvent composition and temperature. Selected response variables were extraction yield, total phenolic content (TPC), total flavonoid content (TFC), total carotenoid content (TCC), antioxidant (ABTS), and anti-inflammatory (LOX) activities, and anti-acetylcholinesterase (AChE) inhibitory capacity. According to the desirability function, the optimal conditions were 100% ethanol and 180°C with a 0.87 desirability value. Next, the anti-butyrylcholinesterase enzyme (BChE), reactive oxygen species (ROS), and reactive nitrogen species (RNS) inhibition as well as cytotoxicity in HK-2, THP-1 monocytes, and SH-5YSY neuroblastoma cell lines were studied for the TE extract obtained under optimized conditions. The optimum TE extract provided the following results: extraction yield (36.25%), TPC (92.09 mg GAE/g extract), TFC (4.4 mg QE/g extract), TCC (107.15 mg CE/g extract), antioxidant capacity (ABTS, IC50 = 6.33 mg/ml extract), LOX (IC50 = 48.3 mg/ml extract), and AChE (IC50 = 97.46 mg/ml extract), and showed no toxicity at concentration up to 120 μg/ml extract for all the tested cell lines. Finally, chemical characterization by liquid chromatography-tandem mass spectrometry (UHPLC-q-TOF-MS/MS) of the optimum TE extract exhibited an important presence of hydroxycinnamic acid derivatives and other phenolic acids as well as quercetin hexoside and rutin, as main metabolites responsible for the observed biological properties. All these results suggested that TE, which represents between 8 and 15% of the total fruit, could become a promising natural by-product with a potential “multitarget” activity against Alzheimer's disease.
format	article
author	Zully Jimena Suárez-Montenegro Zully Jimena Suárez-Montenegro Diego Ballesteros-Vivas Diego Ballesteros-Vivas Rocío Gallego Alberto Valdés Jose David Sánchez-Martínez Fabián Parada-Alfonso Elena Ibáñez Alejandro Cifuentes
author_facet	Zully Jimena Suárez-Montenegro Zully Jimena Suárez-Montenegro Diego Ballesteros-Vivas Diego Ballesteros-Vivas Rocío Gallego Alberto Valdés Jose David Sánchez-Martínez Fabián Parada-Alfonso Elena Ibáñez Alejandro Cifuentes
author_sort	Zully Jimena Suárez-Montenegro
title	Neuroprotective Potential of Tamarillo (Cyphomandra betacea) Epicarp Extracts Obtained by Sustainable Extraction Process
title_short	Neuroprotective Potential of Tamarillo (Cyphomandra betacea) Epicarp Extracts Obtained by Sustainable Extraction Process
title_full	Neuroprotective Potential of Tamarillo (Cyphomandra betacea) Epicarp Extracts Obtained by Sustainable Extraction Process
title_fullStr	Neuroprotective Potential of Tamarillo (Cyphomandra betacea) Epicarp Extracts Obtained by Sustainable Extraction Process
title_full_unstemmed	Neuroprotective Potential of Tamarillo (Cyphomandra betacea) Epicarp Extracts Obtained by Sustainable Extraction Process
title_sort	neuroprotective potential of tamarillo (cyphomandra betacea) epicarp extracts obtained by sustainable extraction process
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/d9573b0a87d84d9bacfc049626534c7c
work_keys_str_mv	AT zullyjimenasuarezmontenegro neuroprotectivepotentialoftamarillocyphomandrabetaceaepicarpextractsobtainedbysustainableextractionprocess AT zullyjimenasuarezmontenegro neuroprotectivepotentialoftamarillocyphomandrabetaceaepicarpextractsobtainedbysustainableextractionprocess AT diegoballesterosvivas neuroprotectivepotentialoftamarillocyphomandrabetaceaepicarpextractsobtainedbysustainableextractionprocess AT diegoballesterosvivas neuroprotectivepotentialoftamarillocyphomandrabetaceaepicarpextractsobtainedbysustainableextractionprocess AT rociogallego neuroprotectivepotentialoftamarillocyphomandrabetaceaepicarpextractsobtainedbysustainableextractionprocess AT albertovaldes neuroprotectivepotentialoftamarillocyphomandrabetaceaepicarpextractsobtainedbysustainableextractionprocess AT josedavidsanchezmartinez neuroprotectivepotentialoftamarillocyphomandrabetaceaepicarpextractsobtainedbysustainableextractionprocess AT fabianparadaalfonso neuroprotectivepotentialoftamarillocyphomandrabetaceaepicarpextractsobtainedbysustainableextractionprocess AT elenaibanez neuroprotectivepotentialoftamarillocyphomandrabetaceaepicarpextractsobtainedbysustainableextractionprocess AT alejandrocifuentes neuroprotectivepotentialoftamarillocyphomandrabetaceaepicarpextractsobtainedbysustainableextractionprocess
_version_	1718428525546962944

Neuroprotective Potential of Tamarillo (Cyphomandra betacea) Epicarp Extracts Obtained by Sustainable Extraction Process

Ejemplares similares