New Provisional Function of OmpA from <italic toggle="yes">Acinetobacter</italic> sp. Strain SA01 Based on Environmental Challenges

ABSTRACT An outer membrane protein A (OmpA) from Acinetobacter sp. strain SA01 was identified and characterized in-depth based on the structural and functional characteristics already known of its homologues. In silico structural studies showed that this protein can be a slow porin, binds to peptido...

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Autores principales:	Shahab Shahryari, Mahbubeh Talaee, Kamahldin Haghbeen, Lorenz Adrian, Hojatollah Vali, Hossein Shahbani Zahiri, Kambiz Akbari Noghabi
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2021
Materias:	Acinetobacter sp. SA01 phenol OmpA emulsifying ability porin oxidative stress Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/d957bf659b3b40ac868b996df49ae4aa
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spelling	oai:doaj.org-article:d957bf659b3b40ac868b996df49ae4aa2021-12-02T19:36:37ZNew Provisional Function of OmpA from <italic toggle="yes">Acinetobacter</italic> sp. Strain SA01 Based on Environmental Challenges10.1128/mSystems.01175-202379-5077https://doaj.org/article/d957bf659b3b40ac868b996df49ae4aa2021-02-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.01175-20https://doaj.org/toc/2379-5077ABSTRACT An outer membrane protein A (OmpA) from Acinetobacter sp. strain SA01 was identified and characterized in-depth based on the structural and functional characteristics already known of its homologues. In silico structural studies showed that this protein can be a slow porin, binds to peptidoglycan, and exhibits emulsifying properties. Characterization of the recombinant SA01-OmpA, based on its emulsifying properties, represented its promising potentials in biotechnology. Also, the presence of SA01-OmpA in outer membrane vesicles (OMV) and biofilm showed that this protein, like its homologues in Acinetobacter baumannii, can be secreted into the extracellular environment through OMVs and play a role in the formation of biofilm. After ensuring the correct selection of the protein of interest, the role of oxidative stress induced by cell nutritional parameters (utilization of specific carbon sources) on the expression level of OmpA was carefully studied. For this purpose, the oxidative stress level of SA01 cell cultures in the presence of three nonrelevant carbon sources (sodium acetate, ethanol, and phenol) was examined under each condition. High expression of SA01-OmpA in ethanol- and phenol-fed cells with higher levels of oxidative stress than acetate suggested that oxidative stress could be a substantial factor in the regulation of SA01-OmpA expression. The significant association of SA01-OmpA expression with the levels of oxidative stress induced by cadmium and H2O2, with oxidative stress-inducing properties and lack of nutritional value, confirmed that the cells tend to harness their capacities with a possible increase in OmpA production. Collectively, this study suggests a homeostasis role for OmpA in Acinetobacter sp. SA01 under oxidative stress besides assuming many other roles hitherto attributed to this protein. IMPORTANCE Acinetobacter OmpA is known as a multifaceted protein with multiple functions, including emulsifying properties. Bioemulsifiers are surface-active compounds that can disperse hydrophobic compounds in water and help increase the bioavailability of hydrophobic hydrocarbons to be used by degrading microorganisms. In this study, an OmpA from Acinetobacter sp. SA01 was identified and introduced as an emulsifier with a higher emulsifying capacity than Pseudomonas aeruginosa rhamnolipid. We also showed that the expression of this protein is not dependent on the nutritional requirements but is more influenced by the oxidative stress caused by stressors. This finding, along with the structural role of this protein as a slow porin or its role in OMV biogenesis and biofilm formation, suggests that this protein can play an important role in maintaining cellular homeostasis under oxidative stress conditions. Altogether, the present study provides a new perspective on the functional performance of Acinetobacter OmpA, which can be used both to optimize its production as an emulsifier and a target in the treatment of multidrug-resistant strains.Shahab ShahryariMahbubeh TalaeeKamahldin HaghbeenLorenz AdrianHojatollah ValiHossein Shahbani ZahiriKambiz Akbari NoghabiAmerican Society for MicrobiologyarticleAcinetobacter sp. SA01phenolOmpAemulsifying abilityporinoxidative stressMicrobiologyQR1-502ENmSystems, Vol 6, Iss 1 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Acinetobacter sp. SA01 phenol OmpA emulsifying ability porin oxidative stress Microbiology QR1-502
spellingShingle	Acinetobacter sp. SA01 phenol OmpA emulsifying ability porin oxidative stress Microbiology QR1-502 Shahab Shahryari Mahbubeh Talaee Kamahldin Haghbeen Lorenz Adrian Hojatollah Vali Hossein Shahbani Zahiri Kambiz Akbari Noghabi New Provisional Function of OmpA from <italic toggle="yes">Acinetobacter</italic> sp. Strain SA01 Based on Environmental Challenges
description	ABSTRACT An outer membrane protein A (OmpA) from Acinetobacter sp. strain SA01 was identified and characterized in-depth based on the structural and functional characteristics already known of its homologues. In silico structural studies showed that this protein can be a slow porin, binds to peptidoglycan, and exhibits emulsifying properties. Characterization of the recombinant SA01-OmpA, based on its emulsifying properties, represented its promising potentials in biotechnology. Also, the presence of SA01-OmpA in outer membrane vesicles (OMV) and biofilm showed that this protein, like its homologues in Acinetobacter baumannii, can be secreted into the extracellular environment through OMVs and play a role in the formation of biofilm. After ensuring the correct selection of the protein of interest, the role of oxidative stress induced by cell nutritional parameters (utilization of specific carbon sources) on the expression level of OmpA was carefully studied. For this purpose, the oxidative stress level of SA01 cell cultures in the presence of three nonrelevant carbon sources (sodium acetate, ethanol, and phenol) was examined under each condition. High expression of SA01-OmpA in ethanol- and phenol-fed cells with higher levels of oxidative stress than acetate suggested that oxidative stress could be a substantial factor in the regulation of SA01-OmpA expression. The significant association of SA01-OmpA expression with the levels of oxidative stress induced by cadmium and H2O2, with oxidative stress-inducing properties and lack of nutritional value, confirmed that the cells tend to harness their capacities with a possible increase in OmpA production. Collectively, this study suggests a homeostasis role for OmpA in Acinetobacter sp. SA01 under oxidative stress besides assuming many other roles hitherto attributed to this protein. IMPORTANCE Acinetobacter OmpA is known as a multifaceted protein with multiple functions, including emulsifying properties. Bioemulsifiers are surface-active compounds that can disperse hydrophobic compounds in water and help increase the bioavailability of hydrophobic hydrocarbons to be used by degrading microorganisms. In this study, an OmpA from Acinetobacter sp. SA01 was identified and introduced as an emulsifier with a higher emulsifying capacity than Pseudomonas aeruginosa rhamnolipid. We also showed that the expression of this protein is not dependent on the nutritional requirements but is more influenced by the oxidative stress caused by stressors. This finding, along with the structural role of this protein as a slow porin or its role in OMV biogenesis and biofilm formation, suggests that this protein can play an important role in maintaining cellular homeostasis under oxidative stress conditions. Altogether, the present study provides a new perspective on the functional performance of Acinetobacter OmpA, which can be used both to optimize its production as an emulsifier and a target in the treatment of multidrug-resistant strains.
format	article
author	Shahab Shahryari Mahbubeh Talaee Kamahldin Haghbeen Lorenz Adrian Hojatollah Vali Hossein Shahbani Zahiri Kambiz Akbari Noghabi
author_facet	Shahab Shahryari Mahbubeh Talaee Kamahldin Haghbeen Lorenz Adrian Hojatollah Vali Hossein Shahbani Zahiri Kambiz Akbari Noghabi
author_sort	Shahab Shahryari
title	New Provisional Function of OmpA from <italic toggle="yes">Acinetobacter</italic> sp. Strain SA01 Based on Environmental Challenges
title_short	New Provisional Function of OmpA from <italic toggle="yes">Acinetobacter</italic> sp. Strain SA01 Based on Environmental Challenges
title_full	New Provisional Function of OmpA from <italic toggle="yes">Acinetobacter</italic> sp. Strain SA01 Based on Environmental Challenges
title_fullStr	New Provisional Function of OmpA from <italic toggle="yes">Acinetobacter</italic> sp. Strain SA01 Based on Environmental Challenges
title_full_unstemmed	New Provisional Function of OmpA from <italic toggle="yes">Acinetobacter</italic> sp. Strain SA01 Based on Environmental Challenges
title_sort	new provisional function of ompa from <italic toggle="yes">acinetobacter</italic> sp. strain sa01 based on environmental challenges
publisher	American Society for Microbiology
publishDate	2021
url	https://doaj.org/article/d957bf659b3b40ac868b996df49ae4aa
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New Provisional Function of OmpA from <italic toggle="yes">Acinetobacter</italic> sp. Strain SA01 Based on Environmental Challenges

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