In vivo and in vitro ageing results in accumulation of de novo copy number variations in bulls

Abstract We have identified de novo copy number variations (CNVs) generated in bulls as they age. Blood samples from eight bulls were collected and SNP arrayed in a prospective design over 30 months allowing us to differentiate de novo CNVs from constant CNVs that are present throughout the sampling...

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Autores principales:	Tamas Revay, Olutobi Oluwole, Tom Kroetsch, W. Allan King
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d95869c3f8c64faaad808040a8aea46b
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spelling	oai:doaj.org-article:d95869c3f8c64faaad808040a8aea46b2021-12-02T15:05:26ZIn vivo and in vitro ageing results in accumulation of de novo copy number variations in bulls10.1038/s41598-017-01793-22045-2322https://doaj.org/article/d95869c3f8c64faaad808040a8aea46b2017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01793-2https://doaj.org/toc/2045-2322Abstract We have identified de novo copy number variations (CNVs) generated in bulls as they age. Blood samples from eight bulls were collected and SNP arrayed in a prospective design over 30 months allowing us to differentiate de novo CNVs from constant CNVs that are present throughout the sampling period. Quite remarkably, the total number of CNVs doubled over the 30-month period, as we observed an almost equal number of de novo and constant CNVs (107 and 111, respectively, i.e. 49% and 51%). Twice as many de novo CNVs emerged during the second half of the sampling schedule as in the first part. It suggests a dynamic generation of de novo CNVs in the bovine genome that becomes more frequent as the age of the animal progresses. In a second experiment de novo CNVs were detected through in vitro ageing of bovine fibroblasts by sampling passage #5, #15 and #25. De novo CNVs also became more frequent, but the proportion of them was only ~25% of the total number of CNVs (21 out of 85). Temporal generation of de novo CNVs resulted in increasing genome coverage. Genes and quantitative trait loci overlapping de novo CNVs were further investigated for ageing related functions.Tamas RevayOlutobi OluwoleTom KroetschW. Allan KingNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tamas Revay Olutobi Oluwole Tom Kroetsch W. Allan King In vivo and in vitro ageing results in accumulation of de novo copy number variations in bulls
description	Abstract We have identified de novo copy number variations (CNVs) generated in bulls as they age. Blood samples from eight bulls were collected and SNP arrayed in a prospective design over 30 months allowing us to differentiate de novo CNVs from constant CNVs that are present throughout the sampling period. Quite remarkably, the total number of CNVs doubled over the 30-month period, as we observed an almost equal number of de novo and constant CNVs (107 and 111, respectively, i.e. 49% and 51%). Twice as many de novo CNVs emerged during the second half of the sampling schedule as in the first part. It suggests a dynamic generation of de novo CNVs in the bovine genome that becomes more frequent as the age of the animal progresses. In a second experiment de novo CNVs were detected through in vitro ageing of bovine fibroblasts by sampling passage #5, #15 and #25. De novo CNVs also became more frequent, but the proportion of them was only ~25% of the total number of CNVs (21 out of 85). Temporal generation of de novo CNVs resulted in increasing genome coverage. Genes and quantitative trait loci overlapping de novo CNVs were further investigated for ageing related functions.
format	article
author	Tamas Revay Olutobi Oluwole Tom Kroetsch W. Allan King
author_facet	Tamas Revay Olutobi Oluwole Tom Kroetsch W. Allan King
author_sort	Tamas Revay
title	In vivo and in vitro ageing results in accumulation of de novo copy number variations in bulls
title_short	In vivo and in vitro ageing results in accumulation of de novo copy number variations in bulls
title_full	In vivo and in vitro ageing results in accumulation of de novo copy number variations in bulls
title_fullStr	In vivo and in vitro ageing results in accumulation of de novo copy number variations in bulls
title_full_unstemmed	In vivo and in vitro ageing results in accumulation of de novo copy number variations in bulls
title_sort	in vivo and in vitro ageing results in accumulation of de novo copy number variations in bulls
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/d95869c3f8c64faaad808040a8aea46b
work_keys_str_mv	AT tamasrevay invivoandinvitroageingresultsinaccumulationofdenovocopynumbervariationsinbulls AT olutobioluwole invivoandinvitroageingresultsinaccumulationofdenovocopynumbervariationsinbulls AT tomkroetsch invivoandinvitroageingresultsinaccumulationofdenovocopynumbervariationsinbulls AT wallanking invivoandinvitroageingresultsinaccumulationofdenovocopynumbervariationsinbulls
_version_	1718388860915810304

In vivo and in vitro ageing results in accumulation of de novo copy number variations in bulls

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