Interfacing aptamers, nanoparticles and graphene in a hierarchical structure for highly selective detection of biomolecules in OECT devices

Abstract In several biomedical applications, the detection of biomarkers demands high sensitivity, selectivity and easy-to-use devices. Organic electrochemical transistors (OECTs) represent a promising class of devices combining a minimal invasiveness and good signal transduction. However, OECTs lac...

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Autores principales: Carlotta Peruzzi, Silvia Battistoni, Daniela Montesarchio, Matteo Cocuzza, Simone Luigi Marasso, Alessio Verna, Laura Pasquardini, Roberto Verucchi, Lucrezia Aversa, Victor Erokhin, Pasquale D’Angelo, Salvatore Iannotta
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Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/d95decbdcc994a2a81f2205040d57be1
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spelling oai:doaj.org-article:d95decbdcc994a2a81f2205040d57be12021-12-02T13:41:34ZInterfacing aptamers, nanoparticles and graphene in a hierarchical structure for highly selective detection of biomolecules in OECT devices10.1038/s41598-021-88546-42045-2322https://doaj.org/article/d95decbdcc994a2a81f2205040d57be12021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88546-4https://doaj.org/toc/2045-2322Abstract In several biomedical applications, the detection of biomarkers demands high sensitivity, selectivity and easy-to-use devices. Organic electrochemical transistors (OECTs) represent a promising class of devices combining a minimal invasiveness and good signal transduction. However, OECTs lack of intrinsic selectivity that should be implemented by specific approaches to make them well suitable for biomedical applications. Here, we report on a biosensor in which selectivity and a high sensitivity are achieved by interfacing, in an OECT architecture, a novel gate electrode based on aptamers, Au nanoparticles and graphene hierarchically organized to optimize the final response. The fabricated biosensor performs state of the art limit of detection monitoring biomolecules, such as thrombin-with a limit of detection in the picomolar range (≤ 5 pM) and a very good selectivity even in presence of supraphysiological concentrations of Bovine Serum Albumin (BSA-1mM). These accomplishments are the final result of the gate hierarchic structure that reduces sterich indrance that could contrast the recognition events and minimizes false positive, because of the low affinity of graphene towards the physiological environment. Since our approach can be easily applied to a large variety of different biomarkers, we envisage a relevant potential for a large series of different biomedical applications.Carlotta PeruzziSilvia BattistoniDaniela MontesarchioMatteo CocuzzaSimone Luigi MarassoAlessio VernaLaura PasquardiniRoberto VerucchiLucrezia AversaVictor ErokhinPasquale D’AngeloSalvatore IannottaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Carlotta Peruzzi
Silvia Battistoni
Daniela Montesarchio
Matteo Cocuzza
Simone Luigi Marasso
Alessio Verna
Laura Pasquardini
Roberto Verucchi
Lucrezia Aversa
Victor Erokhin
Pasquale D’Angelo
Salvatore Iannotta
Interfacing aptamers, nanoparticles and graphene in a hierarchical structure for highly selective detection of biomolecules in OECT devices
description Abstract In several biomedical applications, the detection of biomarkers demands high sensitivity, selectivity and easy-to-use devices. Organic electrochemical transistors (OECTs) represent a promising class of devices combining a minimal invasiveness and good signal transduction. However, OECTs lack of intrinsic selectivity that should be implemented by specific approaches to make them well suitable for biomedical applications. Here, we report on a biosensor in which selectivity and a high sensitivity are achieved by interfacing, in an OECT architecture, a novel gate electrode based on aptamers, Au nanoparticles and graphene hierarchically organized to optimize the final response. The fabricated biosensor performs state of the art limit of detection monitoring biomolecules, such as thrombin-with a limit of detection in the picomolar range (≤ 5 pM) and a very good selectivity even in presence of supraphysiological concentrations of Bovine Serum Albumin (BSA-1mM). These accomplishments are the final result of the gate hierarchic structure that reduces sterich indrance that could contrast the recognition events and minimizes false positive, because of the low affinity of graphene towards the physiological environment. Since our approach can be easily applied to a large variety of different biomarkers, we envisage a relevant potential for a large series of different biomedical applications.
format article
author Carlotta Peruzzi
Silvia Battistoni
Daniela Montesarchio
Matteo Cocuzza
Simone Luigi Marasso
Alessio Verna
Laura Pasquardini
Roberto Verucchi
Lucrezia Aversa
Victor Erokhin
Pasquale D’Angelo
Salvatore Iannotta
author_facet Carlotta Peruzzi
Silvia Battistoni
Daniela Montesarchio
Matteo Cocuzza
Simone Luigi Marasso
Alessio Verna
Laura Pasquardini
Roberto Verucchi
Lucrezia Aversa
Victor Erokhin
Pasquale D’Angelo
Salvatore Iannotta
author_sort Carlotta Peruzzi
title Interfacing aptamers, nanoparticles and graphene in a hierarchical structure for highly selective detection of biomolecules in OECT devices
title_short Interfacing aptamers, nanoparticles and graphene in a hierarchical structure for highly selective detection of biomolecules in OECT devices
title_full Interfacing aptamers, nanoparticles and graphene in a hierarchical structure for highly selective detection of biomolecules in OECT devices
title_fullStr Interfacing aptamers, nanoparticles and graphene in a hierarchical structure for highly selective detection of biomolecules in OECT devices
title_full_unstemmed Interfacing aptamers, nanoparticles and graphene in a hierarchical structure for highly selective detection of biomolecules in OECT devices
title_sort interfacing aptamers, nanoparticles and graphene in a hierarchical structure for highly selective detection of biomolecules in oect devices
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/d95decbdcc994a2a81f2205040d57be1
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