Interfacing aptamers, nanoparticles and graphene in a hierarchical structure for highly selective detection of biomolecules in OECT devices
Abstract In several biomedical applications, the detection of biomarkers demands high sensitivity, selectivity and easy-to-use devices. Organic electrochemical transistors (OECTs) represent a promising class of devices combining a minimal invasiveness and good signal transduction. However, OECTs lac...
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2021
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oai:doaj.org-article:d95decbdcc994a2a81f2205040d57be12021-12-02T13:41:34ZInterfacing aptamers, nanoparticles and graphene in a hierarchical structure for highly selective detection of biomolecules in OECT devices10.1038/s41598-021-88546-42045-2322https://doaj.org/article/d95decbdcc994a2a81f2205040d57be12021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88546-4https://doaj.org/toc/2045-2322Abstract In several biomedical applications, the detection of biomarkers demands high sensitivity, selectivity and easy-to-use devices. Organic electrochemical transistors (OECTs) represent a promising class of devices combining a minimal invasiveness and good signal transduction. However, OECTs lack of intrinsic selectivity that should be implemented by specific approaches to make them well suitable for biomedical applications. Here, we report on a biosensor in which selectivity and a high sensitivity are achieved by interfacing, in an OECT architecture, a novel gate electrode based on aptamers, Au nanoparticles and graphene hierarchically organized to optimize the final response. The fabricated biosensor performs state of the art limit of detection monitoring biomolecules, such as thrombin-with a limit of detection in the picomolar range (≤ 5 pM) and a very good selectivity even in presence of supraphysiological concentrations of Bovine Serum Albumin (BSA-1mM). These accomplishments are the final result of the gate hierarchic structure that reduces sterich indrance that could contrast the recognition events and minimizes false positive, because of the low affinity of graphene towards the physiological environment. Since our approach can be easily applied to a large variety of different biomarkers, we envisage a relevant potential for a large series of different biomedical applications.Carlotta PeruzziSilvia BattistoniDaniela MontesarchioMatteo CocuzzaSimone Luigi MarassoAlessio VernaLaura PasquardiniRoberto VerucchiLucrezia AversaVictor ErokhinPasquale D’AngeloSalvatore IannottaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021) |
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Medicine R Science Q Carlotta Peruzzi Silvia Battistoni Daniela Montesarchio Matteo Cocuzza Simone Luigi Marasso Alessio Verna Laura Pasquardini Roberto Verucchi Lucrezia Aversa Victor Erokhin Pasquale D’Angelo Salvatore Iannotta Interfacing aptamers, nanoparticles and graphene in a hierarchical structure for highly selective detection of biomolecules in OECT devices |
description |
Abstract In several biomedical applications, the detection of biomarkers demands high sensitivity, selectivity and easy-to-use devices. Organic electrochemical transistors (OECTs) represent a promising class of devices combining a minimal invasiveness and good signal transduction. However, OECTs lack of intrinsic selectivity that should be implemented by specific approaches to make them well suitable for biomedical applications. Here, we report on a biosensor in which selectivity and a high sensitivity are achieved by interfacing, in an OECT architecture, a novel gate electrode based on aptamers, Au nanoparticles and graphene hierarchically organized to optimize the final response. The fabricated biosensor performs state of the art limit of detection monitoring biomolecules, such as thrombin-with a limit of detection in the picomolar range (≤ 5 pM) and a very good selectivity even in presence of supraphysiological concentrations of Bovine Serum Albumin (BSA-1mM). These accomplishments are the final result of the gate hierarchic structure that reduces sterich indrance that could contrast the recognition events and minimizes false positive, because of the low affinity of graphene towards the physiological environment. Since our approach can be easily applied to a large variety of different biomarkers, we envisage a relevant potential for a large series of different biomedical applications. |
format |
article |
author |
Carlotta Peruzzi Silvia Battistoni Daniela Montesarchio Matteo Cocuzza Simone Luigi Marasso Alessio Verna Laura Pasquardini Roberto Verucchi Lucrezia Aversa Victor Erokhin Pasquale D’Angelo Salvatore Iannotta |
author_facet |
Carlotta Peruzzi Silvia Battistoni Daniela Montesarchio Matteo Cocuzza Simone Luigi Marasso Alessio Verna Laura Pasquardini Roberto Verucchi Lucrezia Aversa Victor Erokhin Pasquale D’Angelo Salvatore Iannotta |
author_sort |
Carlotta Peruzzi |
title |
Interfacing aptamers, nanoparticles and graphene in a hierarchical structure for highly selective detection of biomolecules in OECT devices |
title_short |
Interfacing aptamers, nanoparticles and graphene in a hierarchical structure for highly selective detection of biomolecules in OECT devices |
title_full |
Interfacing aptamers, nanoparticles and graphene in a hierarchical structure for highly selective detection of biomolecules in OECT devices |
title_fullStr |
Interfacing aptamers, nanoparticles and graphene in a hierarchical structure for highly selective detection of biomolecules in OECT devices |
title_full_unstemmed |
Interfacing aptamers, nanoparticles and graphene in a hierarchical structure for highly selective detection of biomolecules in OECT devices |
title_sort |
interfacing aptamers, nanoparticles and graphene in a hierarchical structure for highly selective detection of biomolecules in oect devices |
publisher |
Nature Portfolio |
publishDate |
2021 |
url |
https://doaj.org/article/d95decbdcc994a2a81f2205040d57be1 |
work_keys_str_mv |
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