Complete chloroplast genome sequence and phylogenetic analysis of wasabi (Eutrema japonicum) and its relatives

Abstract In Japan, two Eutrema species, wasabi (Eutrema japonicum, the important traditional Japanese condiment) and yuriwasabi (E. tenue), have been recognized as endemic species. We sequenced complete chloroplast (cp) genomes of seven wasabi and yuriwasabi accessions from Japan to study their phyl...

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Autores principales:	Natsuko Haga, Masaaki Kobayashi, Nana Michiki, Tomoyuki Takano, Fujio Baba, Keiko Kobayashi, Hajime Ohyanagi, Jun Ohgane, Kentaro Yano, Kyoko Yamane
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d96912763871401abf103d226cd73434
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spelling	oai:doaj.org-article:d96912763871401abf103d226cd734342021-12-02T15:10:02ZComplete chloroplast genome sequence and phylogenetic analysis of wasabi (Eutrema japonicum) and its relatives10.1038/s41598-019-49667-z2045-2322https://doaj.org/article/d96912763871401abf103d226cd734342019-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-49667-zhttps://doaj.org/toc/2045-2322Abstract In Japan, two Eutrema species, wasabi (Eutrema japonicum, the important traditional Japanese condiment) and yuriwasabi (E. tenue), have been recognized as endemic species. We sequenced complete chloroplast (cp) genomes of seven wasabi and yuriwasabi accessions from Japan to study their phylogeny and evolution, using molecular dating of species divergence. Phylogenetic analyses of the complete cp DNA of these two Japanese species and five other Eurasian Eutrema species revealed that wasabi and yuriwasabi did not form a monophyletic group. One yuriwasabi accession (Gifu) formed a clade with E. yunnanense from China, indicating that this accession should be considered as a different species from the other yuriwasabi accessions. We reveal that Japanese Eutrema species diverged from the ‘E. yunnanense–yuriwasabi (Gifu)’ clade approximately 1.3 million years ago (Mya), suggesting that the connection between Japan and the Eurasian continent has existed more recently than the Quaternary period. The abundance of cp sequence data in this study also allowed the detection of genetic differentiation among wasabi cultivars. The two polymorphic sites detected between ‘Fujidaruma’ and ‘Shimane No.3’ were used to develop genotyping markers. The cp genome information provided here will thus inform the evolutionary histories of Japanese Eutrema species and help in genotyping wasabi cultivars.Natsuko HagaMasaaki KobayashiNana MichikiTomoyuki TakanoFujio BabaKeiko KobayashiHajime OhyanagiJun OhganeKentaro YanoKyoko YamaneNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-10 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Natsuko Haga Masaaki Kobayashi Nana Michiki Tomoyuki Takano Fujio Baba Keiko Kobayashi Hajime Ohyanagi Jun Ohgane Kentaro Yano Kyoko Yamane Complete chloroplast genome sequence and phylogenetic analysis of wasabi (Eutrema japonicum) and its relatives
description	Abstract In Japan, two Eutrema species, wasabi (Eutrema japonicum, the important traditional Japanese condiment) and yuriwasabi (E. tenue), have been recognized as endemic species. We sequenced complete chloroplast (cp) genomes of seven wasabi and yuriwasabi accessions from Japan to study their phylogeny and evolution, using molecular dating of species divergence. Phylogenetic analyses of the complete cp DNA of these two Japanese species and five other Eurasian Eutrema species revealed that wasabi and yuriwasabi did not form a monophyletic group. One yuriwasabi accession (Gifu) formed a clade with E. yunnanense from China, indicating that this accession should be considered as a different species from the other yuriwasabi accessions. We reveal that Japanese Eutrema species diverged from the ‘E. yunnanense–yuriwasabi (Gifu)’ clade approximately 1.3 million years ago (Mya), suggesting that the connection between Japan and the Eurasian continent has existed more recently than the Quaternary period. The abundance of cp sequence data in this study also allowed the detection of genetic differentiation among wasabi cultivars. The two polymorphic sites detected between ‘Fujidaruma’ and ‘Shimane No.3’ were used to develop genotyping markers. The cp genome information provided here will thus inform the evolutionary histories of Japanese Eutrema species and help in genotyping wasabi cultivars.
format	article
author	Natsuko Haga Masaaki Kobayashi Nana Michiki Tomoyuki Takano Fujio Baba Keiko Kobayashi Hajime Ohyanagi Jun Ohgane Kentaro Yano Kyoko Yamane
author_facet	Natsuko Haga Masaaki Kobayashi Nana Michiki Tomoyuki Takano Fujio Baba Keiko Kobayashi Hajime Ohyanagi Jun Ohgane Kentaro Yano Kyoko Yamane
author_sort	Natsuko Haga
title	Complete chloroplast genome sequence and phylogenetic analysis of wasabi (Eutrema japonicum) and its relatives
title_short	Complete chloroplast genome sequence and phylogenetic analysis of wasabi (Eutrema japonicum) and its relatives
title_full	Complete chloroplast genome sequence and phylogenetic analysis of wasabi (Eutrema japonicum) and its relatives
title_fullStr	Complete chloroplast genome sequence and phylogenetic analysis of wasabi (Eutrema japonicum) and its relatives
title_full_unstemmed	Complete chloroplast genome sequence and phylogenetic analysis of wasabi (Eutrema japonicum) and its relatives
title_sort	complete chloroplast genome sequence and phylogenetic analysis of wasabi (eutrema japonicum) and its relatives
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/d96912763871401abf103d226cd73434
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Complete chloroplast genome sequence and phylogenetic analysis of wasabi (Eutrema japonicum) and its relatives

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