Three-dimensional in vivo analysis of water uptake and translocation in maize roots by fast neutron tomography

Abstract Root water uptake is an essential process for terrestrial plants that strongly affects the spatiotemporal distribution of water in vegetated soil. Fast neutron tomography is a recently established non-invasive imaging technique capable to capture the 3D architecture of root systems in situ...

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Autores principales:	Christian Tötzke, Nikolay Kardjilov, André Hilger, Nicole Rudolph-Mohr, Ingo Manke, Sascha E. Oswald
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d96c6940f65f4fe4b0b14a9ca5c19c49
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spelling	oai:doaj.org-article:d96c6940f65f4fe4b0b14a9ca5c19c492021-12-02T15:52:55ZThree-dimensional in vivo analysis of water uptake and translocation in maize roots by fast neutron tomography10.1038/s41598-021-90062-42045-2322https://doaj.org/article/d96c6940f65f4fe4b0b14a9ca5c19c492021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90062-4https://doaj.org/toc/2045-2322Abstract Root water uptake is an essential process for terrestrial plants that strongly affects the spatiotemporal distribution of water in vegetated soil. Fast neutron tomography is a recently established non-invasive imaging technique capable to capture the 3D architecture of root systems in situ and even allows for tracking of three-dimensional water flow in soil and roots. We present an in vivo analysis of local water uptake and transport by roots of soil-grown maize plants—for the first time measured in a three-dimensional time-resolved manner. Using deuterated water as tracer in infiltration experiments, we visualized soil imbibition, local root uptake, and tracked the transport of deuterated water throughout the fibrous root system for a day and night situation. This revealed significant differences in water transport between different root types. The primary root was the preferred water transport path in the 13-days-old plants while seminal roots of comparable size and length contributed little to plant water supply. The results underline the unique potential of fast neutron tomography to provide time-resolved 3D in vivo information on the water uptake and transport dynamics of plant root systems, thus contributing to a better understanding of the complex interactions of plant, soil and water.Christian TötzkeNikolay KardjilovAndré HilgerNicole Rudolph-MohrIngo MankeSascha E. OswaldNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Christian Tötzke Nikolay Kardjilov André Hilger Nicole Rudolph-Mohr Ingo Manke Sascha E. Oswald Three-dimensional in vivo analysis of water uptake and translocation in maize roots by fast neutron tomography
description	Abstract Root water uptake is an essential process for terrestrial plants that strongly affects the spatiotemporal distribution of water in vegetated soil. Fast neutron tomography is a recently established non-invasive imaging technique capable to capture the 3D architecture of root systems in situ and even allows for tracking of three-dimensional water flow in soil and roots. We present an in vivo analysis of local water uptake and transport by roots of soil-grown maize plants—for the first time measured in a three-dimensional time-resolved manner. Using deuterated water as tracer in infiltration experiments, we visualized soil imbibition, local root uptake, and tracked the transport of deuterated water throughout the fibrous root system for a day and night situation. This revealed significant differences in water transport between different root types. The primary root was the preferred water transport path in the 13-days-old plants while seminal roots of comparable size and length contributed little to plant water supply. The results underline the unique potential of fast neutron tomography to provide time-resolved 3D in vivo information on the water uptake and transport dynamics of plant root systems, thus contributing to a better understanding of the complex interactions of plant, soil and water.
format	article
author	Christian Tötzke Nikolay Kardjilov André Hilger Nicole Rudolph-Mohr Ingo Manke Sascha E. Oswald
author_facet	Christian Tötzke Nikolay Kardjilov André Hilger Nicole Rudolph-Mohr Ingo Manke Sascha E. Oswald
author_sort	Christian Tötzke
title	Three-dimensional in vivo analysis of water uptake and translocation in maize roots by fast neutron tomography
title_short	Three-dimensional in vivo analysis of water uptake and translocation in maize roots by fast neutron tomography
title_full	Three-dimensional in vivo analysis of water uptake and translocation in maize roots by fast neutron tomography
title_fullStr	Three-dimensional in vivo analysis of water uptake and translocation in maize roots by fast neutron tomography
title_full_unstemmed	Three-dimensional in vivo analysis of water uptake and translocation in maize roots by fast neutron tomography
title_sort	three-dimensional in vivo analysis of water uptake and translocation in maize roots by fast neutron tomography
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d96c6940f65f4fe4b0b14a9ca5c19c49
work_keys_str_mv	AT christiantotzke threedimensionalinvivoanalysisofwateruptakeandtranslocationinmaizerootsbyfastneutrontomography AT nikolaykardjilov threedimensionalinvivoanalysisofwateruptakeandtranslocationinmaizerootsbyfastneutrontomography AT andrehilger threedimensionalinvivoanalysisofwateruptakeandtranslocationinmaizerootsbyfastneutrontomography AT nicolerudolphmohr threedimensionalinvivoanalysisofwateruptakeandtranslocationinmaizerootsbyfastneutrontomography AT ingomanke threedimensionalinvivoanalysisofwateruptakeandtranslocationinmaizerootsbyfastneutrontomography AT saschaeoswald threedimensionalinvivoanalysisofwateruptakeandtranslocationinmaizerootsbyfastneutrontomography
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Three-dimensional in vivo analysis of water uptake and translocation in maize roots by fast neutron tomography

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