Transcription reinitiation by recycling RNA polymerase that diffuses on DNA after releasing terminated RNA

Bacterial transcription is terminated when RNA polymerases encounter terminator sequences. Using a single-molecule fluorescence assay, here the authors show that the release of transcript RNA precedes RNA polymerase dissociation and that the remaining RNA polymerase can reinitiate at nearby promoter...

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Bibliographic Details
Main Authors: Wooyoung Kang, Kook Sun Ha, Heesoo Uhm, Kyuhyong Park, Ja Yil Lee, Sungchul Hohng, Changwon Kang
Format: article
Language:EN
Published: Nature Portfolio 2020
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Online Access:https://doaj.org/article/d97e30411e7f425eab5086b9c09f7d1c
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Summary:Bacterial transcription is terminated when RNA polymerases encounter terminator sequences. Using a single-molecule fluorescence assay, here the authors show that the release of transcript RNA precedes RNA polymerase dissociation and that the remaining RNA polymerase can reinitiate at nearby promoters.