Whole genomic sequencing based genotyping reveals a specific X3 sublineage restricted to Mexico and related with multidrug resistance

Abstract Whole genome sequencing (WGS) has been shown to be superior to traditional procedures of genotyping in tuberculosis (TB), nevertheless, reports of its use in drug resistant TB (DR-TB) isolates circulating in Mexico, are practically unknown. Considering the above the main of this work was to...

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Autores principales:	Ana Cristina Jiménez-Ruano, Carlos Francisco Madrazo-Moya, Irving Cancino-Muñoz, Paulina M. Mejía-Ponce, Cuauhtémoc Licona-Cassani, Iñaki Comas, Raquel Muñiz-Salazar, Roberto Zenteno-Cuevas
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d981ec0aba9448918343c9047c6a2288
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spelling	oai:doaj.org-article:d981ec0aba9448918343c9047c6a22882021-12-02T13:56:47ZWhole genomic sequencing based genotyping reveals a specific X3 sublineage restricted to Mexico and related with multidrug resistance10.1038/s41598-020-80919-52045-2322https://doaj.org/article/d981ec0aba9448918343c9047c6a22882021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80919-5https://doaj.org/toc/2045-2322Abstract Whole genome sequencing (WGS) has been shown to be superior to traditional procedures of genotyping in tuberculosis (TB), nevertheless, reports of its use in drug resistant TB (DR-TB) isolates circulating in Mexico, are practically unknown. Considering the above the main of this work was to identify and characterize the lineages and genomic transmission clusters present in 67 DR-TB isolates circulating in southeastern Mexico. The results show the presence of three major lineages: L1 (3%), L2 (3%) and L4 (94%), the last one included 16 sublineages. Sublineage 4.1.1.3 (X3) was predominant in 18 (27%) of the isolates, including one genomic cluster, formed by eleven multidrug resistant isolates and sharing the SIT 3278, which seems to be restricted to Mexico. By the use of WGS, it was possible to identify the high prevalence of L4 and a high number of sublineages circulating in the region, also was recognized the presence of a novel X3 sublineage, formed exclusively by multidrug resistant isolates and with restrictive circulation in Mexico for at least the past 17 years.Ana Cristina Jiménez-RuanoCarlos Francisco Madrazo-MoyaIrving Cancino-MuñozPaulina M. Mejía-PonceCuauhtémoc Licona-CassaniIñaki ComasRaquel Muñiz-SalazarRoberto Zenteno-CuevasNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ana Cristina Jiménez-Ruano Carlos Francisco Madrazo-Moya Irving Cancino-Muñoz Paulina M. Mejía-Ponce Cuauhtémoc Licona-Cassani Iñaki Comas Raquel Muñiz-Salazar Roberto Zenteno-Cuevas Whole genomic sequencing based genotyping reveals a specific X3 sublineage restricted to Mexico and related with multidrug resistance
description	Abstract Whole genome sequencing (WGS) has been shown to be superior to traditional procedures of genotyping in tuberculosis (TB), nevertheless, reports of its use in drug resistant TB (DR-TB) isolates circulating in Mexico, are practically unknown. Considering the above the main of this work was to identify and characterize the lineages and genomic transmission clusters present in 67 DR-TB isolates circulating in southeastern Mexico. The results show the presence of three major lineages: L1 (3%), L2 (3%) and L4 (94%), the last one included 16 sublineages. Sublineage 4.1.1.3 (X3) was predominant in 18 (27%) of the isolates, including one genomic cluster, formed by eleven multidrug resistant isolates and sharing the SIT 3278, which seems to be restricted to Mexico. By the use of WGS, it was possible to identify the high prevalence of L4 and a high number of sublineages circulating in the region, also was recognized the presence of a novel X3 sublineage, formed exclusively by multidrug resistant isolates and with restrictive circulation in Mexico for at least the past 17 years.
format	article
author	Ana Cristina Jiménez-Ruano Carlos Francisco Madrazo-Moya Irving Cancino-Muñoz Paulina M. Mejía-Ponce Cuauhtémoc Licona-Cassani Iñaki Comas Raquel Muñiz-Salazar Roberto Zenteno-Cuevas
author_facet	Ana Cristina Jiménez-Ruano Carlos Francisco Madrazo-Moya Irving Cancino-Muñoz Paulina M. Mejía-Ponce Cuauhtémoc Licona-Cassani Iñaki Comas Raquel Muñiz-Salazar Roberto Zenteno-Cuevas
author_sort	Ana Cristina Jiménez-Ruano
title	Whole genomic sequencing based genotyping reveals a specific X3 sublineage restricted to Mexico and related with multidrug resistance
title_short	Whole genomic sequencing based genotyping reveals a specific X3 sublineage restricted to Mexico and related with multidrug resistance
title_full	Whole genomic sequencing based genotyping reveals a specific X3 sublineage restricted to Mexico and related with multidrug resistance
title_fullStr	Whole genomic sequencing based genotyping reveals a specific X3 sublineage restricted to Mexico and related with multidrug resistance
title_full_unstemmed	Whole genomic sequencing based genotyping reveals a specific X3 sublineage restricted to Mexico and related with multidrug resistance
title_sort	whole genomic sequencing based genotyping reveals a specific x3 sublineage restricted to mexico and related with multidrug resistance
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d981ec0aba9448918343c9047c6a2288
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Whole genomic sequencing based genotyping reveals a specific X3 sublineage restricted to Mexico and related with multidrug resistance

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