DNase Treatment Improves Viral Enrichment in Agricultural Soil Viromes

Viromics is becoming an increasingly popular method for characterizing soil viral communities. DNase treatment of the viral size fraction prior to DNA extraction is meant to reduce contaminating free DNA and is a common step within viromics protocols to ensure that sequences are of viral origin.

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Autores principales:	Jackson W. Sorensen, Laura A. Zinke, Anneliek M. ter Horst, Christian Santos-Medellín, Alena Schroeder, Joanne B. Emerson
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2021
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/d982701bba2147549f9be758c9a92217
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spelling	oai:doaj.org-article:d982701bba2147549f9be758c9a922172021-12-02T19:44:32ZDNase Treatment Improves Viral Enrichment in Agricultural Soil Viromes2379-507710.1128/mSystems.00614-21https://doaj.org/article/d982701bba2147549f9be758c9a922172021-10-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.00614-21https://doaj.org/toc/2379-5077 Viromics is becoming an increasingly popular method for characterizing soil viral communities. DNase treatment of the viral size fraction prior to DNA extraction is meant to reduce contaminating free DNA and is a common step within viromics protocols to ensure that sequences are of viral origin.Jackson W. SorensenLaura A. ZinkeAnneliek M. ter HorstChristian Santos-MedellínAlena SchroederJoanne B. EmersonAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmSystems, Vol 6, Iss 5 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Jackson W. Sorensen Laura A. Zinke Anneliek M. ter Horst Christian Santos-Medellín Alena Schroeder Joanne B. Emerson DNase Treatment Improves Viral Enrichment in Agricultural Soil Viromes
description	Viromics is becoming an increasingly popular method for characterizing soil viral communities. DNase treatment of the viral size fraction prior to DNA extraction is meant to reduce contaminating free DNA and is a common step within viromics protocols to ensure that sequences are of viral origin.
format	article
author	Jackson W. Sorensen Laura A. Zinke Anneliek M. ter Horst Christian Santos-Medellín Alena Schroeder Joanne B. Emerson
author_facet	Jackson W. Sorensen Laura A. Zinke Anneliek M. ter Horst Christian Santos-Medellín Alena Schroeder Joanne B. Emerson
author_sort	Jackson W. Sorensen
title	DNase Treatment Improves Viral Enrichment in Agricultural Soil Viromes
title_short	DNase Treatment Improves Viral Enrichment in Agricultural Soil Viromes
title_full	DNase Treatment Improves Viral Enrichment in Agricultural Soil Viromes
title_fullStr	DNase Treatment Improves Viral Enrichment in Agricultural Soil Viromes
title_full_unstemmed	DNase Treatment Improves Viral Enrichment in Agricultural Soil Viromes
title_sort	dnase treatment improves viral enrichment in agricultural soil viromes
publisher	American Society for Microbiology
publishDate	2021
url	https://doaj.org/article/d982701bba2147549f9be758c9a92217
work_keys_str_mv	AT jacksonwsorensen dnasetreatmentimprovesviralenrichmentinagriculturalsoilviromes AT lauraazinke dnasetreatmentimprovesviralenrichmentinagriculturalsoilviromes AT anneliekmterhorst dnasetreatmentimprovesviralenrichmentinagriculturalsoilviromes AT christiansantosmedellin dnasetreatmentimprovesviralenrichmentinagriculturalsoilviromes AT alenaschroeder dnasetreatmentimprovesviralenrichmentinagriculturalsoilviromes AT joannebemerson dnasetreatmentimprovesviralenrichmentinagriculturalsoilviromes
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DNase Treatment Improves Viral Enrichment in Agricultural Soil Viromes

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