Synovial Fluid Cytokines, Chemokines and MMP Levels in Osteoarthritis Patients with Knee Pain Display a Profile Similar to Many Rheumatoid Arthritis Patients

Background: There are currently no effective disease-modifying drugs to prevent cartilage loss in osteoarthritis and synovial fluid is a potentially valuable source of biomarkers to understand the pathogenesis of different types of arthritis and identify drug responsiveness. The aim of this study wa...

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Autores principales:	Richard T. Meehan, Elizabeth A. Regan, Eric D. Hoffman, Molly L. Wolf, Mary T. Gill, James L. Crooks, Prashant J. Parmar, Richard A. Scheuring, John C. Hill, Karin A. Pacheco, Vijaya Knight
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	synovial fluid biomarkers cytokines osteoarthritis Medicine R
Acceso en línea:	https://doaj.org/article/d985528fdd974d5eb22c806dcdbba844
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spelling	oai:doaj.org-article:d985528fdd974d5eb22c806dcdbba8442021-11-11T17:39:27ZSynovial Fluid Cytokines, Chemokines and MMP Levels in Osteoarthritis Patients with Knee Pain Display a Profile Similar to Many Rheumatoid Arthritis Patients10.3390/jcm102150272077-0383https://doaj.org/article/d985528fdd974d5eb22c806dcdbba8442021-10-01T00:00:00Zhttps://www.mdpi.com/2077-0383/10/21/5027https://doaj.org/toc/2077-0383Background: There are currently no effective disease-modifying drugs to prevent cartilage loss in osteoarthritis and synovial fluid is a potentially valuable source of biomarkers to understand the pathogenesis of different types of arthritis and identify drug responsiveness. The aim of this study was to compare the differences between SF cytokines and other proteins in patients with OA (<i>n</i> = 21) to those with RA (<i>n</i> = 27) and normal knees (<i>n</i> = 3). Methods: SF was obtained using ultrasound (US) guidance and an external pneumatic compression device. RA patients were categorized as active (<i>n</i> = 20) or controlled (<i>n</i> = 7) based upon SF white blood cell counts (> or <300 cells/mm<sup>3</sup>). Samples were cryopreserved and analyzed by multiplex fluorescent bead assays (Luminex). Between-group differences of 16 separate biomarker proteins were identified using ANOVA on log10-transformed concentrations with <i>p</i> values adjusted for multiple testing. Results: Only six biomarkers were significantly higher in SF from active RA compared to OA—TNF-α, IL-1-β IL-7, MMP-1, MMP-2, and MMP-3. Only MMP-8 levels in RA patients correlated with SF WBC counts (<i>p</i> < 0.0001). Among OA patients, simultaneous SF IL-4, IL-6, IL-8, and IL-15 levels were higher than serum levels, whereas MMP-8, MMP-9, and IL-18 levels were higher in serum (<i>p</i> < 0.05). Conclusion: These results support the growing evidence that OA patients have a pro-inflammatory/catabolic SF environment. SF biomarker analysis using multiplex testing and US guidance may distinguish OA phenotypes and identify treatment options based upon targeted inflammatory pathways similar to patients with RA.Richard T. MeehanElizabeth A. ReganEric D. HoffmanMolly L. WolfMary T. GillJames L. CrooksPrashant J. ParmarRichard A. ScheuringJohn C. HillKarin A. PachecoVijaya KnightMDPI AGarticlesynovial fluidbiomarkerscytokinesosteoarthritisMedicineRENJournal of Clinical Medicine, Vol 10, Iss 5027, p 5027 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	synovial fluid biomarkers cytokines osteoarthritis Medicine R
spellingShingle	synovial fluid biomarkers cytokines osteoarthritis Medicine R Richard T. Meehan Elizabeth A. Regan Eric D. Hoffman Molly L. Wolf Mary T. Gill James L. Crooks Prashant J. Parmar Richard A. Scheuring John C. Hill Karin A. Pacheco Vijaya Knight Synovial Fluid Cytokines, Chemokines and MMP Levels in Osteoarthritis Patients with Knee Pain Display a Profile Similar to Many Rheumatoid Arthritis Patients
description	Background: There are currently no effective disease-modifying drugs to prevent cartilage loss in osteoarthritis and synovial fluid is a potentially valuable source of biomarkers to understand the pathogenesis of different types of arthritis and identify drug responsiveness. The aim of this study was to compare the differences between SF cytokines and other proteins in patients with OA (<i>n</i> = 21) to those with RA (<i>n</i> = 27) and normal knees (<i>n</i> = 3). Methods: SF was obtained using ultrasound (US) guidance and an external pneumatic compression device. RA patients were categorized as active (<i>n</i> = 20) or controlled (<i>n</i> = 7) based upon SF white blood cell counts (> or <300 cells/mm<sup>3</sup>). Samples were cryopreserved and analyzed by multiplex fluorescent bead assays (Luminex). Between-group differences of 16 separate biomarker proteins were identified using ANOVA on log10-transformed concentrations with <i>p</i> values adjusted for multiple testing. Results: Only six biomarkers were significantly higher in SF from active RA compared to OA—TNF-α, IL-1-β IL-7, MMP-1, MMP-2, and MMP-3. Only MMP-8 levels in RA patients correlated with SF WBC counts (<i>p</i> < 0.0001). Among OA patients, simultaneous SF IL-4, IL-6, IL-8, and IL-15 levels were higher than serum levels, whereas MMP-8, MMP-9, and IL-18 levels were higher in serum (<i>p</i> < 0.05). Conclusion: These results support the growing evidence that OA patients have a pro-inflammatory/catabolic SF environment. SF biomarker analysis using multiplex testing and US guidance may distinguish OA phenotypes and identify treatment options based upon targeted inflammatory pathways similar to patients with RA.
format	article
author	Richard T. Meehan Elizabeth A. Regan Eric D. Hoffman Molly L. Wolf Mary T. Gill James L. Crooks Prashant J. Parmar Richard A. Scheuring John C. Hill Karin A. Pacheco Vijaya Knight
author_facet	Richard T. Meehan Elizabeth A. Regan Eric D. Hoffman Molly L. Wolf Mary T. Gill James L. Crooks Prashant J. Parmar Richard A. Scheuring John C. Hill Karin A. Pacheco Vijaya Knight
author_sort	Richard T. Meehan
title	Synovial Fluid Cytokines, Chemokines and MMP Levels in Osteoarthritis Patients with Knee Pain Display a Profile Similar to Many Rheumatoid Arthritis Patients
title_short	Synovial Fluid Cytokines, Chemokines and MMP Levels in Osteoarthritis Patients with Knee Pain Display a Profile Similar to Many Rheumatoid Arthritis Patients
title_full	Synovial Fluid Cytokines, Chemokines and MMP Levels in Osteoarthritis Patients with Knee Pain Display a Profile Similar to Many Rheumatoid Arthritis Patients
title_fullStr	Synovial Fluid Cytokines, Chemokines and MMP Levels in Osteoarthritis Patients with Knee Pain Display a Profile Similar to Many Rheumatoid Arthritis Patients
title_full_unstemmed	Synovial Fluid Cytokines, Chemokines and MMP Levels in Osteoarthritis Patients with Knee Pain Display a Profile Similar to Many Rheumatoid Arthritis Patients
title_sort	synovial fluid cytokines, chemokines and mmp levels in osteoarthritis patients with knee pain display a profile similar to many rheumatoid arthritis patients
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/d985528fdd974d5eb22c806dcdbba844
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Synovial Fluid Cytokines, Chemokines and MMP Levels in Osteoarthritis Patients with Knee Pain Display a Profile Similar to Many Rheumatoid Arthritis Patients

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