Enrichment and Quantification of Epitope-specific CD4+ T Lymphocytes using Ferromagnetic Iron-gold and Nickel Nanowires

Abstract Epitope-specific CD4+ T lymphocytes were magnetically enriched using ferromagnetic Ni and Fe-Au nanowires coated with a monomer containing a major histocompatibility complex class II-bound peptide epitope (pMHCII). The enriched lymphocytes were subsequently quantified using fluorescence-act...

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Autores principales:	Daniel E. Shore, Thamotharampillai Dileepan, Jaime F. Modiano, Marc K. Jenkins, Bethanie J. H. Stadler
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Magnetic Enrichment Paramagnetic Beads Major Histocompatibility Complex Class II (MHCII) Tetramer Solution NiSi Nanowires Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d9899e4430bb42ccb37827b9dc8de565
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spelling	oai:doaj.org-article:d9899e4430bb42ccb37827b9dc8de5652021-12-02T15:07:46ZEnrichment and Quantification of Epitope-specific CD4+ T Lymphocytes using Ferromagnetic Iron-gold and Nickel Nanowires10.1038/s41598-018-33910-02045-2322https://doaj.org/article/d9899e4430bb42ccb37827b9dc8de5652018-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-33910-0https://doaj.org/toc/2045-2322Abstract Epitope-specific CD4+ T lymphocytes were magnetically enriched using ferromagnetic Ni and Fe-Au nanowires coated with a monomer containing a major histocompatibility complex class II-bound peptide epitope (pMHCII). The enriched lymphocytes were subsequently quantified using fluorescence-activated cell sorting (FACS). This was the first use of magnetic nanowires for cell sorting using FACS, and improvements in both specificity and fluorescent signal strength were predicted due to higher particle moments and lengths than conventional paramagnetic beads. Three different types of nanowires (Ni, Fe with Au tip and Fe-Au multilayers) were made by electrodeposition. Ni nanowires separated fewer T cells than Au tipped Fe nanowires, likely because Ni has a lower magnetic moment than Fe. Fe-Au multilayer nanowires separated more T cells than Au-tipped Fe nanowires because there was more monomer per nanowire. Also, increasing the amount of monomer increased the number of CD4+ cells separated. Compared to conventional paramagnetic beads, the nanowires had lower specificity for CD4+ T cells, but had stronger fluorescent signals due to more fluorophores per particle. This results in broader FACS baseline separation between the positive and negative cells, which is useful to detect T cells, even those with lower binding affinity for pMHCII ligands.Daniel E. ShoreThamotharampillai DileepanJaime F. ModianoMarc K. JenkinsBethanie J. H. StadlerNature PortfolioarticleMagnetic EnrichmentParamagnetic BeadsMajor Histocompatibility Complex Class II (MHCII)Tetramer SolutionNiSi NanowiresMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-7 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Magnetic Enrichment Paramagnetic Beads Major Histocompatibility Complex Class II (MHCII) Tetramer Solution NiSi Nanowires Medicine R Science Q
spellingShingle	Magnetic Enrichment Paramagnetic Beads Major Histocompatibility Complex Class II (MHCII) Tetramer Solution NiSi Nanowires Medicine R Science Q Daniel E. Shore Thamotharampillai Dileepan Jaime F. Modiano Marc K. Jenkins Bethanie J. H. Stadler Enrichment and Quantification of Epitope-specific CD4+ T Lymphocytes using Ferromagnetic Iron-gold and Nickel Nanowires
description	Abstract Epitope-specific CD4+ T lymphocytes were magnetically enriched using ferromagnetic Ni and Fe-Au nanowires coated with a monomer containing a major histocompatibility complex class II-bound peptide epitope (pMHCII). The enriched lymphocytes were subsequently quantified using fluorescence-activated cell sorting (FACS). This was the first use of magnetic nanowires for cell sorting using FACS, and improvements in both specificity and fluorescent signal strength were predicted due to higher particle moments and lengths than conventional paramagnetic beads. Three different types of nanowires (Ni, Fe with Au tip and Fe-Au multilayers) were made by electrodeposition. Ni nanowires separated fewer T cells than Au tipped Fe nanowires, likely because Ni has a lower magnetic moment than Fe. Fe-Au multilayer nanowires separated more T cells than Au-tipped Fe nanowires because there was more monomer per nanowire. Also, increasing the amount of monomer increased the number of CD4+ cells separated. Compared to conventional paramagnetic beads, the nanowires had lower specificity for CD4+ T cells, but had stronger fluorescent signals due to more fluorophores per particle. This results in broader FACS baseline separation between the positive and negative cells, which is useful to detect T cells, even those with lower binding affinity for pMHCII ligands.
format	article
author	Daniel E. Shore Thamotharampillai Dileepan Jaime F. Modiano Marc K. Jenkins Bethanie J. H. Stadler
author_facet	Daniel E. Shore Thamotharampillai Dileepan Jaime F. Modiano Marc K. Jenkins Bethanie J. H. Stadler
author_sort	Daniel E. Shore
title	Enrichment and Quantification of Epitope-specific CD4+ T Lymphocytes using Ferromagnetic Iron-gold and Nickel Nanowires
title_short	Enrichment and Quantification of Epitope-specific CD4+ T Lymphocytes using Ferromagnetic Iron-gold and Nickel Nanowires
title_full	Enrichment and Quantification of Epitope-specific CD4+ T Lymphocytes using Ferromagnetic Iron-gold and Nickel Nanowires
title_fullStr	Enrichment and Quantification of Epitope-specific CD4+ T Lymphocytes using Ferromagnetic Iron-gold and Nickel Nanowires
title_full_unstemmed	Enrichment and Quantification of Epitope-specific CD4+ T Lymphocytes using Ferromagnetic Iron-gold and Nickel Nanowires
title_sort	enrichment and quantification of epitope-specific cd4+ t lymphocytes using ferromagnetic iron-gold and nickel nanowires
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/d9899e4430bb42ccb37827b9dc8de565
work_keys_str_mv	AT danieleshore enrichmentandquantificationofepitopespecificcd4tlymphocytesusingferromagneticirongoldandnickelnanowires AT thamotharampillaidileepan enrichmentandquantificationofepitopespecificcd4tlymphocytesusingferromagneticirongoldandnickelnanowires AT jaimefmodiano enrichmentandquantificationofepitopespecificcd4tlymphocytesusingferromagneticirongoldandnickelnanowires AT marckjenkins enrichmentandquantificationofepitopespecificcd4tlymphocytesusingferromagneticirongoldandnickelnanowires AT bethaniejhstadler enrichmentandquantificationofepitopespecificcd4tlymphocytesusingferromagneticirongoldandnickelnanowires
_version_	1718388419769401344

Enrichment and Quantification of Epitope-specific CD4+ T Lymphocytes using Ferromagnetic Iron-gold and Nickel Nanowires

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