Sirtuin-3 Protects Cochlear Hair Cells Against Noise-Induced Damage via the Superoxide Dismutase 2/Reactive Oxygen Species Signaling Pathway

Mitochondrial oxidative stress is involved in hair cell damage caused by noise-induced hearing loss (NIHL). Sirtuin-3 (SIRT3) plays an important role in hair cell survival by regulating mitochondrial function; however, the role of SIRT3 in NIHL is unknown. In this study, we used 3-TYP to inhibit SIR...

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Autores principales:	Wenqi Liang, Chunli Zhao, Zhongrui Chen, Zijing Yang, Ke Liu, Shusheng Gong
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	SIRT3 SOD2 noise-induced hearing loss viral transduction oxidative stress Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/d98ad4db57e4464d84c1d56d5906f339
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spelling	oai:doaj.org-article:d98ad4db57e4464d84c1d56d5906f3392021-11-18T09:51:25ZSirtuin-3 Protects Cochlear Hair Cells Against Noise-Induced Damage via the Superoxide Dismutase 2/Reactive Oxygen Species Signaling Pathway2296-634X10.3389/fcell.2021.766512https://doaj.org/article/d98ad4db57e4464d84c1d56d5906f3392021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcell.2021.766512/fullhttps://doaj.org/toc/2296-634XMitochondrial oxidative stress is involved in hair cell damage caused by noise-induced hearing loss (NIHL). Sirtuin-3 (SIRT3) plays an important role in hair cell survival by regulating mitochondrial function; however, the role of SIRT3 in NIHL is unknown. In this study, we used 3-TYP to inhibit SIRT3 and found that this inhibition aggravated oxidative damage in the hair cells of mice with NIHL. Moreover, 3-TYP reduced the enzymatic activity and deacetylation levels of superoxide dismutase 2 (SOD2). Subsequently, we administered adeno-associated virus-SIRT3 to the posterior semicircular canals and found that SIRT3 overexpression significantly attenuated hair cell injury and that this protective effect of SIRT3 could be blocked by 2-methoxyestradiol, a SOD2 inhibitor. These findings suggest that insufficient SIRT3/SOD2 signaling leads to mitochondrial oxidative damage resulting in hair cell injury in NIHL. Thus, ameliorating noise-induced mitochondrial redox imbalance by intervening in the SIRT3/SOD2 signaling pathway may be a new therapeutic target for hair cell injury.Wenqi LiangChunli ZhaoZhongrui ChenZijing YangKe LiuShusheng GongFrontiers Media S.A.articleSIRT3SOD2noise-induced hearing lossviral transductionoxidative stressBiology (General)QH301-705.5ENFrontiers in Cell and Developmental Biology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	SIRT3 SOD2 noise-induced hearing loss viral transduction oxidative stress Biology (General) QH301-705.5
spellingShingle	SIRT3 SOD2 noise-induced hearing loss viral transduction oxidative stress Biology (General) QH301-705.5 Wenqi Liang Chunli Zhao Zhongrui Chen Zijing Yang Ke Liu Shusheng Gong Sirtuin-3 Protects Cochlear Hair Cells Against Noise-Induced Damage via the Superoxide Dismutase 2/Reactive Oxygen Species Signaling Pathway
description	Mitochondrial oxidative stress is involved in hair cell damage caused by noise-induced hearing loss (NIHL). Sirtuin-3 (SIRT3) plays an important role in hair cell survival by regulating mitochondrial function; however, the role of SIRT3 in NIHL is unknown. In this study, we used 3-TYP to inhibit SIRT3 and found that this inhibition aggravated oxidative damage in the hair cells of mice with NIHL. Moreover, 3-TYP reduced the enzymatic activity and deacetylation levels of superoxide dismutase 2 (SOD2). Subsequently, we administered adeno-associated virus-SIRT3 to the posterior semicircular canals and found that SIRT3 overexpression significantly attenuated hair cell injury and that this protective effect of SIRT3 could be blocked by 2-methoxyestradiol, a SOD2 inhibitor. These findings suggest that insufficient SIRT3/SOD2 signaling leads to mitochondrial oxidative damage resulting in hair cell injury in NIHL. Thus, ameliorating noise-induced mitochondrial redox imbalance by intervening in the SIRT3/SOD2 signaling pathway may be a new therapeutic target for hair cell injury.
format	article
author	Wenqi Liang Chunli Zhao Zhongrui Chen Zijing Yang Ke Liu Shusheng Gong
author_facet	Wenqi Liang Chunli Zhao Zhongrui Chen Zijing Yang Ke Liu Shusheng Gong
author_sort	Wenqi Liang
title	Sirtuin-3 Protects Cochlear Hair Cells Against Noise-Induced Damage via the Superoxide Dismutase 2/Reactive Oxygen Species Signaling Pathway
title_short	Sirtuin-3 Protects Cochlear Hair Cells Against Noise-Induced Damage via the Superoxide Dismutase 2/Reactive Oxygen Species Signaling Pathway
title_full	Sirtuin-3 Protects Cochlear Hair Cells Against Noise-Induced Damage via the Superoxide Dismutase 2/Reactive Oxygen Species Signaling Pathway
title_fullStr	Sirtuin-3 Protects Cochlear Hair Cells Against Noise-Induced Damage via the Superoxide Dismutase 2/Reactive Oxygen Species Signaling Pathway
title_full_unstemmed	Sirtuin-3 Protects Cochlear Hair Cells Against Noise-Induced Damage via the Superoxide Dismutase 2/Reactive Oxygen Species Signaling Pathway
title_sort	sirtuin-3 protects cochlear hair cells against noise-induced damage via the superoxide dismutase 2/reactive oxygen species signaling pathway
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/d98ad4db57e4464d84c1d56d5906f339
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Sirtuin-3 Protects Cochlear Hair Cells Against Noise-Induced Damage via the Superoxide Dismutase 2/Reactive Oxygen Species Signaling Pathway

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