Spatial distribution of conspecific genotypes within chimeras of the branching coral Stylophora pistillata

Abstract Chimerism is a coalescence of conspecific genotypes. Although common in nature, fundamental knowledge, such as the spatial distribution of the genotypes within chimeras, is lacking. Hence, we investigated the spatial distribution of conspecific genotypes within the brooding coral Stylophora...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Gabriele Guerrini, Dor Shefy, Jacob Douek, Nadav Shashar, Tamar L. Goulet, Baruch Rinkevich
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d98b0f77ccc940f7bef309ac75ad17f4
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d98b0f77ccc940f7bef309ac75ad17f4
record_format	dspace
spelling	oai:doaj.org-article:d98b0f77ccc940f7bef309ac75ad17f42021-11-21T12:17:59ZSpatial distribution of conspecific genotypes within chimeras of the branching coral Stylophora pistillata10.1038/s41598-021-00981-52045-2322https://doaj.org/article/d98b0f77ccc940f7bef309ac75ad17f42021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-00981-5https://doaj.org/toc/2045-2322Abstract Chimerism is a coalescence of conspecific genotypes. Although common in nature, fundamental knowledge, such as the spatial distribution of the genotypes within chimeras, is lacking. Hence, we investigated the spatial distribution of conspecific genotypes within the brooding coral Stylophora pistillata, a common species throughout the Indo-Pacific and Red Sea. From eight gravid colonies, we collected planula larvae that settled in aggregates, forming 2–3 partner chimeras. Coral chimeras grew in situ for up to 25 months. Nine chimeras (8 kin, 1 non-related genotypes) were sectioned into 7–17 fragments (6–26 polyps/fragment), and genotyped using eight microsatellite loci. The discrimination power of each microsatellite-locus was evaluated with 330 ‘artificial chimeras,’ made by mixing DNA from three different S. pistillata genotypes in pairwise combinations. In 68% of ‘artificial chimeras,’ the second genotype was detected if it constituted 5–30% of the chimera. Analyses of S. pistillata chimeras revealed that: (a) chimerism is a long-term state; (b) conspecifics were intermixed (not separate from one another); (c) disproportionate distribution of the conspecifics occurred; (d) cryptic chimerism (chimerism not detected via a given microsatellite) existed, alluding to the underestimation of chimerism in nature. Mixed chimerism may affect ecological/physiological outcomes for a chimera, especially in clonal organisms, and challenges the concept of individuality, affecting our understanding of the unit of selection.Gabriele GuerriniDor ShefyJacob DouekNadav ShasharTamar L. GouletBaruch RinkevichNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Gabriele Guerrini Dor Shefy Jacob Douek Nadav Shashar Tamar L. Goulet Baruch Rinkevich Spatial distribution of conspecific genotypes within chimeras of the branching coral Stylophora pistillata
description	Abstract Chimerism is a coalescence of conspecific genotypes. Although common in nature, fundamental knowledge, such as the spatial distribution of the genotypes within chimeras, is lacking. Hence, we investigated the spatial distribution of conspecific genotypes within the brooding coral Stylophora pistillata, a common species throughout the Indo-Pacific and Red Sea. From eight gravid colonies, we collected planula larvae that settled in aggregates, forming 2–3 partner chimeras. Coral chimeras grew in situ for up to 25 months. Nine chimeras (8 kin, 1 non-related genotypes) were sectioned into 7–17 fragments (6–26 polyps/fragment), and genotyped using eight microsatellite loci. The discrimination power of each microsatellite-locus was evaluated with 330 ‘artificial chimeras,’ made by mixing DNA from three different S. pistillata genotypes in pairwise combinations. In 68% of ‘artificial chimeras,’ the second genotype was detected if it constituted 5–30% of the chimera. Analyses of S. pistillata chimeras revealed that: (a) chimerism is a long-term state; (b) conspecifics were intermixed (not separate from one another); (c) disproportionate distribution of the conspecifics occurred; (d) cryptic chimerism (chimerism not detected via a given microsatellite) existed, alluding to the underestimation of chimerism in nature. Mixed chimerism may affect ecological/physiological outcomes for a chimera, especially in clonal organisms, and challenges the concept of individuality, affecting our understanding of the unit of selection.
format	article
author	Gabriele Guerrini Dor Shefy Jacob Douek Nadav Shashar Tamar L. Goulet Baruch Rinkevich
author_facet	Gabriele Guerrini Dor Shefy Jacob Douek Nadav Shashar Tamar L. Goulet Baruch Rinkevich
author_sort	Gabriele Guerrini
title	Spatial distribution of conspecific genotypes within chimeras of the branching coral Stylophora pistillata
title_short	Spatial distribution of conspecific genotypes within chimeras of the branching coral Stylophora pistillata
title_full	Spatial distribution of conspecific genotypes within chimeras of the branching coral Stylophora pistillata
title_fullStr	Spatial distribution of conspecific genotypes within chimeras of the branching coral Stylophora pistillata
title_full_unstemmed	Spatial distribution of conspecific genotypes within chimeras of the branching coral Stylophora pistillata
title_sort	spatial distribution of conspecific genotypes within chimeras of the branching coral stylophora pistillata
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d98b0f77ccc940f7bef309ac75ad17f4
work_keys_str_mv	AT gabrieleguerrini spatialdistributionofconspecificgenotypeswithinchimerasofthebranchingcoralstylophorapistillata AT dorshefy spatialdistributionofconspecificgenotypeswithinchimerasofthebranchingcoralstylophorapistillata AT jacobdouek spatialdistributionofconspecificgenotypeswithinchimerasofthebranchingcoralstylophorapistillata AT nadavshashar spatialdistributionofconspecificgenotypeswithinchimerasofthebranchingcoralstylophorapistillata AT tamarlgoulet spatialdistributionofconspecificgenotypeswithinchimerasofthebranchingcoralstylophorapistillata AT baruchrinkevich spatialdistributionofconspecificgenotypeswithinchimerasofthebranchingcoralstylophorapistillata
_version_	1718419096465309696

Spatial distribution of conspecific genotypes within chimeras of the branching coral Stylophora pistillata

Ejemplares similares