Peptide OPTX-1 From Ornithodoros papillipes Tick Inhibits the pS273R Protease of African Swine Fever Virus

African swine fever virus (ASFV) is a large double-stranded DNA virus and causes high mortality in swine. ASFV can be transmitted by biological vectors, including soft ticks in genus Ornithodoros but not hard ticks. However, the underlying mechanisms evolved in the vectorial capacity of soft ticks a...

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Autores principales:	Jingjing Wang, Mengyao Ji, Bingqian Yuan, Anna Luo, Zhenyuan Jiang, Tengyu Zhu, Yang Liu, Peter Muiruri Kamau, Lin Jin, Ren Lai
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Ornithodoros papillipes African swine fever virus soft ticks pS273R protease vector Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/d98ecd840501467684d49054c1fb4529
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spelling	oai:doaj.org-article:d98ecd840501467684d49054c1fb45292021-12-03T05:21:16ZPeptide OPTX-1 From Ornithodoros papillipes Tick Inhibits the pS273R Protease of African Swine Fever Virus1664-302X10.3389/fmicb.2021.778309https://doaj.org/article/d98ecd840501467684d49054c1fb45292021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.778309/fullhttps://doaj.org/toc/1664-302XAfrican swine fever virus (ASFV) is a large double-stranded DNA virus and causes high mortality in swine. ASFV can be transmitted by biological vectors, including soft ticks in genus Ornithodoros but not hard ticks. However, the underlying mechanisms evolved in the vectorial capacity of soft ticks are not well-understood. Here, we found that a defensin-like peptide toxin OPTX-1 identified from Ornithodoros papillipes inhibits the enzyme activity of the ASFV pS273R protease with a Ki=0.821±0.526μM and shows inhibitory activity on the replication of ASFV. The analogs of OPTX-1 from hard ticks show more inhibitory efficient on pS273R protease. Considering that ticks are blood-sucking animals, we tested the effects of OPTX-1 and its analogs on the coagulation system. At last, top 3D structures represented surface analyses of the binding sites of pS273R with different inhibitors that were obtained by molecular docking based on known structural information. In summary, our study provides evidence that different inhibitory efficiencies between soft tick-derived OPTX-1 and hard tick-derived defensin-like peptides may determine the vector and reservoir competence of ticks.Jingjing WangJingjing WangMengyao JiBingqian YuanAnna LuoZhenyuan JiangZhenyuan JiangTengyu ZhuTengyu ZhuYang LiuYang LiuPeter Muiruri KamauLin JinLin JinRen LaiRen LaiRen LaiRen LaiFrontiers Media S.A.articleOrnithodoros papillipesAfrican swine fever virussoft tickspS273R proteasevectorMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Ornithodoros papillipes African swine fever virus soft ticks pS273R protease vector Microbiology QR1-502
spellingShingle	Ornithodoros papillipes African swine fever virus soft ticks pS273R protease vector Microbiology QR1-502 Jingjing Wang Jingjing Wang Mengyao Ji Bingqian Yuan Anna Luo Zhenyuan Jiang Zhenyuan Jiang Tengyu Zhu Tengyu Zhu Yang Liu Yang Liu Peter Muiruri Kamau Lin Jin Lin Jin Ren Lai Ren Lai Ren Lai Ren Lai Peptide OPTX-1 From Ornithodoros papillipes Tick Inhibits the pS273R Protease of African Swine Fever Virus
description	African swine fever virus (ASFV) is a large double-stranded DNA virus and causes high mortality in swine. ASFV can be transmitted by biological vectors, including soft ticks in genus Ornithodoros but not hard ticks. However, the underlying mechanisms evolved in the vectorial capacity of soft ticks are not well-understood. Here, we found that a defensin-like peptide toxin OPTX-1 identified from Ornithodoros papillipes inhibits the enzyme activity of the ASFV pS273R protease with a Ki=0.821±0.526μM and shows inhibitory activity on the replication of ASFV. The analogs of OPTX-1 from hard ticks show more inhibitory efficient on pS273R protease. Considering that ticks are blood-sucking animals, we tested the effects of OPTX-1 and its analogs on the coagulation system. At last, top 3D structures represented surface analyses of the binding sites of pS273R with different inhibitors that were obtained by molecular docking based on known structural information. In summary, our study provides evidence that different inhibitory efficiencies between soft tick-derived OPTX-1 and hard tick-derived defensin-like peptides may determine the vector and reservoir competence of ticks.
format	article
author	Jingjing Wang Jingjing Wang Mengyao Ji Bingqian Yuan Anna Luo Zhenyuan Jiang Zhenyuan Jiang Tengyu Zhu Tengyu Zhu Yang Liu Yang Liu Peter Muiruri Kamau Lin Jin Lin Jin Ren Lai Ren Lai Ren Lai Ren Lai
author_facet	Jingjing Wang Jingjing Wang Mengyao Ji Bingqian Yuan Anna Luo Zhenyuan Jiang Zhenyuan Jiang Tengyu Zhu Tengyu Zhu Yang Liu Yang Liu Peter Muiruri Kamau Lin Jin Lin Jin Ren Lai Ren Lai Ren Lai Ren Lai
author_sort	Jingjing Wang
title	Peptide OPTX-1 From Ornithodoros papillipes Tick Inhibits the pS273R Protease of African Swine Fever Virus
title_short	Peptide OPTX-1 From Ornithodoros papillipes Tick Inhibits the pS273R Protease of African Swine Fever Virus
title_full	Peptide OPTX-1 From Ornithodoros papillipes Tick Inhibits the pS273R Protease of African Swine Fever Virus
title_fullStr	Peptide OPTX-1 From Ornithodoros papillipes Tick Inhibits the pS273R Protease of African Swine Fever Virus
title_full_unstemmed	Peptide OPTX-1 From Ornithodoros papillipes Tick Inhibits the pS273R Protease of African Swine Fever Virus
title_sort	peptide optx-1 from ornithodoros papillipes tick inhibits the ps273r protease of african swine fever virus
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/d98ecd840501467684d49054c1fb4529
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Peptide OPTX-1 From Ornithodoros papillipes Tick Inhibits the pS273R Protease of African Swine Fever Virus

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