Assessment of Ceragenins in Prevention of Damage to Voice Prostheses Caused by <i>Candida</i> Biofilm Formation
This study aimed to investigate the potential application of ceragenins (CSAs) as new candidacidal agents to prevent biofilm formation on voice prostheses (VPs). The deterioration of the silicone material of VPs is caused by biofilm growth on the device which leads to frequent replacement procedures...
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2021
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oai:doaj.org-article:d98ed3384d94437686cb846398b547422021-11-25T18:37:42ZAssessment of Ceragenins in Prevention of Damage to Voice Prostheses Caused by <i>Candida</i> Biofilm Formation10.3390/pathogens101113712076-0817https://doaj.org/article/d98ed3384d94437686cb846398b547422021-10-01T00:00:00Zhttps://www.mdpi.com/2076-0817/10/11/1371https://doaj.org/toc/2076-0817This study aimed to investigate the potential application of ceragenins (CSAs) as new candidacidal agents to prevent biofilm formation on voice prostheses (VPs). The deterioration of the silicone material of VPs is caused by biofilm growth on the device which leads to frequent replacement procedures and sometimes serious complications. A significant proportion of these failures is caused by <i>Candida</i> species. We found that CSAs have significant candidacidal activities in vitro (MIC; MFC; MBIC), and they effectively eradicate species of yeast responsible for VP failure. Additionally, in our in vitro experimental setting, when different <i>Candida</i> species were subjected to CSA-13 and CSA-131 during 25 passages, no tested <i>Candida</i> strain showed the significant development of resistance. Using liquid chromatography–mass spectrometry (LC-MS), we found that VP immersion in an ethanol solution containing CSA-131 results in silicon impregnation with CSA-131 molecules, and in vitro testing revealed that fungal biofilm formation on such VP surfaces was inhibited by embedded ceragenins. Future in vivo studies will validate the use of ceragenin-coated VP for improvement in the life quality and safety of patients after a total laryngectomy.Jakub SpałekTamara DanilukAdrian GodlewskiPiotr DeptułaUrszula WnorowskaDominika ZiembickaMateusz CieślukKrzysztof FiedorukMichał CiborowskiAdam KrętowskiStanisław GóźdźBonita DurnaśPaul B. SavageSławomir OkłaRobert BuckiMDPI AGarticlecerageninssiliconeimpregnationvoice prosthesesbiofilm<i>Candida</i> spp.MedicineRENPathogens, Vol 10, Iss 1371, p 1371 (2021) |
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DOAJ |
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| topic |
ceragenins silicone impregnation voice prostheses biofilm <i>Candida</i> spp. Medicine R |
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ceragenins silicone impregnation voice prostheses biofilm <i>Candida</i> spp. Medicine R Jakub Spałek Tamara Daniluk Adrian Godlewski Piotr Deptuła Urszula Wnorowska Dominika Ziembicka Mateusz Cieśluk Krzysztof Fiedoruk Michał Ciborowski Adam Krętowski Stanisław Góźdź Bonita Durnaś Paul B. Savage Sławomir Okła Robert Bucki Assessment of Ceragenins in Prevention of Damage to Voice Prostheses Caused by <i>Candida</i> Biofilm Formation |
| description |
This study aimed to investigate the potential application of ceragenins (CSAs) as new candidacidal agents to prevent biofilm formation on voice prostheses (VPs). The deterioration of the silicone material of VPs is caused by biofilm growth on the device which leads to frequent replacement procedures and sometimes serious complications. A significant proportion of these failures is caused by <i>Candida</i> species. We found that CSAs have significant candidacidal activities in vitro (MIC; MFC; MBIC), and they effectively eradicate species of yeast responsible for VP failure. Additionally, in our in vitro experimental setting, when different <i>Candida</i> species were subjected to CSA-13 and CSA-131 during 25 passages, no tested <i>Candida</i> strain showed the significant development of resistance. Using liquid chromatography–mass spectrometry (LC-MS), we found that VP immersion in an ethanol solution containing CSA-131 results in silicon impregnation with CSA-131 molecules, and in vitro testing revealed that fungal biofilm formation on such VP surfaces was inhibited by embedded ceragenins. Future in vivo studies will validate the use of ceragenin-coated VP for improvement in the life quality and safety of patients after a total laryngectomy. |
| format |
article |
| author |
Jakub Spałek Tamara Daniluk Adrian Godlewski Piotr Deptuła Urszula Wnorowska Dominika Ziembicka Mateusz Cieśluk Krzysztof Fiedoruk Michał Ciborowski Adam Krętowski Stanisław Góźdź Bonita Durnaś Paul B. Savage Sławomir Okła Robert Bucki |
| author_facet |
Jakub Spałek Tamara Daniluk Adrian Godlewski Piotr Deptuła Urszula Wnorowska Dominika Ziembicka Mateusz Cieśluk Krzysztof Fiedoruk Michał Ciborowski Adam Krętowski Stanisław Góźdź Bonita Durnaś Paul B. Savage Sławomir Okła Robert Bucki |
| author_sort |
Jakub Spałek |
| title |
Assessment of Ceragenins in Prevention of Damage to Voice Prostheses Caused by <i>Candida</i> Biofilm Formation |
| title_short |
Assessment of Ceragenins in Prevention of Damage to Voice Prostheses Caused by <i>Candida</i> Biofilm Formation |
| title_full |
Assessment of Ceragenins in Prevention of Damage to Voice Prostheses Caused by <i>Candida</i> Biofilm Formation |
| title_fullStr |
Assessment of Ceragenins in Prevention of Damage to Voice Prostheses Caused by <i>Candida</i> Biofilm Formation |
| title_full_unstemmed |
Assessment of Ceragenins in Prevention of Damage to Voice Prostheses Caused by <i>Candida</i> Biofilm Formation |
| title_sort |
assessment of ceragenins in prevention of damage to voice prostheses caused by <i>candida</i> biofilm formation |
| publisher |
MDPI AG |
| publishDate |
2021 |
| url |
https://doaj.org/article/d98ed3384d94437686cb846398b54742 |
| work_keys_str_mv |
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1718410923256840192 |