The Effect of CMCE Propolis Extract Administrations on Interleukin-1 (IL-1) Levels and the Hepatocyte HistopathologicalFindings of Rats’ Induced with CCl4

Introduction:Liver exposure to toxic substances will disrupt the metabolic system. Carbon tetrachloride (CCl4) is a hepatotoxic substance causing a liver damage. Continuous multi-stage countercurrent extraction(CMCE) propolis extract contains active balsamic substances, caffeic acid, phenethyl ester...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Catur Retno Lestari, Titiek Sumarawati, Taufiqurrachman Nasihun
Formato:	article
Lenguaje:	EN
Publicado:	Universitas Sultan Agung Semarang 2020
Materias:	cmce propolis extract, il-1, histopathology hepatic cell Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/d9910d37ce1e4299b60de3aac69cf608
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d9910d37ce1e4299b60de3aac69cf608
record_format	dspace
spelling	oai:doaj.org-article:d9910d37ce1e4299b60de3aac69cf6082021-11-25T13:16:36ZThe Effect of CMCE Propolis Extract Administrations on Interleukin-1 (IL-1) Levels and the Hepatocyte HistopathologicalFindings of Rats’ Induced with CCl42085-15452339-093X10.30659/sainsmed.v11i1.4882https://doaj.org/article/d9910d37ce1e4299b60de3aac69cf6082020-07-01T00:00:00Zhttp://jurnal.unissula.ac.id/index.php/sainsmedika/article/view/4882https://doaj.org/toc/2085-1545https://doaj.org/toc/2339-093XIntroduction:Liver exposure to toxic substances will disrupt the metabolic system. Carbon tetrachloride (CCl4) is a hepatotoxic substance causing a liver damage. Continuous multi-stage countercurrent extraction(CMCE) propolis extract contains active balsamic substances, caffeic acid, phenethyl esters and flavonoids. Objective: to prove the effect of CMCE propolis extract on IL-1 levels and the hepatocyte histopathology findingsof male wistar rats’induced by CCl4. Methods:In the posttest only control group design, the total sample of 28 rats was divided into 4 groups: positive control group (K-0; standard feed); group P-1 (propolis3.6 mg/200 g); P-2 (propolis7.2mg/200gr and P-3 (propolis14.4mg/200g) were respectively given for 14 days,yet those on the 14thday were given CCl4. The IL-1 level was measured with the ELISA method, while the male wistar rats’hepatocyte histopathology findings was determined by the HE staining methods. IL-1 levelswere analyzed usingOne Way Anova test,followed by the Post Hoc LSD test. Hepatic cell histopathology was analyzed usingKruskal Wallis, followed by Mann-Whitney U. Results:Post Hoc LSD analysis shows that the levels of IL-1 in P-1 (983.54 ±19.67), P-2 (841.58±19.47), and P-3 (755.50±31.63) are significantlylower thanthosein K-0 (1131.69±14.91) withp<0.05. Mann-Whitney U analysis shows that the hepatocyte histopathology in the P-1 group (2.55±0.39), P-2 (1.24±0.94) and P-3 (1.20±0.93) is significantlylower thanthatinK-0 (2.94±0.16) with p<0.05. Conclusion: The administration of CMCE propolis extract may reduce IL-1 levels and improve the male wistar rats’hepatic cell histopathologyinduced with carbon tetrachloride (CCl4).Catur Retno LestariTitiek SumarawatiTaufiqurrachman NasihunUniversitas Sultan Agung Semarangarticlecmce propolis extract, il-1, histopathology hepatic cellMedicine (General)R5-920ENSains Medika, Vol 11, Iss 1, Pp 7-13 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	cmce propolis extract, il-1, histopathology hepatic cell Medicine (General) R5-920
spellingShingle	cmce propolis extract, il-1, histopathology hepatic cell Medicine (General) R5-920 Catur Retno Lestari Titiek Sumarawati Taufiqurrachman Nasihun The Effect of CMCE Propolis Extract Administrations on Interleukin-1 (IL-1) Levels and the Hepatocyte HistopathologicalFindings of Rats’ Induced with CCl4
description	Introduction:Liver exposure to toxic substances will disrupt the metabolic system. Carbon tetrachloride (CCl4) is a hepatotoxic substance causing a liver damage. Continuous multi-stage countercurrent extraction(CMCE) propolis extract contains active balsamic substances, caffeic acid, phenethyl esters and flavonoids. Objective: to prove the effect of CMCE propolis extract on IL-1 levels and the hepatocyte histopathology findingsof male wistar rats’induced by CCl4. Methods:In the posttest only control group design, the total sample of 28 rats was divided into 4 groups: positive control group (K-0; standard feed); group P-1 (propolis3.6 mg/200 g); P-2 (propolis7.2mg/200gr and P-3 (propolis14.4mg/200g) were respectively given for 14 days,yet those on the 14thday were given CCl4. The IL-1 level was measured with the ELISA method, while the male wistar rats’hepatocyte histopathology findings was determined by the HE staining methods. IL-1 levelswere analyzed usingOne Way Anova test,followed by the Post Hoc LSD test. Hepatic cell histopathology was analyzed usingKruskal Wallis, followed by Mann-Whitney U. Results:Post Hoc LSD analysis shows that the levels of IL-1 in P-1 (983.54 ±19.67), P-2 (841.58±19.47), and P-3 (755.50±31.63) are significantlylower thanthosein K-0 (1131.69±14.91) withp<0.05. Mann-Whitney U analysis shows that the hepatocyte histopathology in the P-1 group (2.55±0.39), P-2 (1.24±0.94) and P-3 (1.20±0.93) is significantlylower thanthatinK-0 (2.94±0.16) with p<0.05. Conclusion: The administration of CMCE propolis extract may reduce IL-1 levels and improve the male wistar rats’hepatic cell histopathologyinduced with carbon tetrachloride (CCl4).
format	article
author	Catur Retno Lestari Titiek Sumarawati Taufiqurrachman Nasihun
author_facet	Catur Retno Lestari Titiek Sumarawati Taufiqurrachman Nasihun
author_sort	Catur Retno Lestari
title	The Effect of CMCE Propolis Extract Administrations on Interleukin-1 (IL-1) Levels and the Hepatocyte HistopathologicalFindings of Rats’ Induced with CCl4
title_short	The Effect of CMCE Propolis Extract Administrations on Interleukin-1 (IL-1) Levels and the Hepatocyte HistopathologicalFindings of Rats’ Induced with CCl4
title_full	The Effect of CMCE Propolis Extract Administrations on Interleukin-1 (IL-1) Levels and the Hepatocyte HistopathologicalFindings of Rats’ Induced with CCl4
title_fullStr	The Effect of CMCE Propolis Extract Administrations on Interleukin-1 (IL-1) Levels and the Hepatocyte HistopathologicalFindings of Rats’ Induced with CCl4
title_full_unstemmed	The Effect of CMCE Propolis Extract Administrations on Interleukin-1 (IL-1) Levels and the Hepatocyte HistopathologicalFindings of Rats’ Induced with CCl4
title_sort	effect of cmce propolis extract administrations on interleukin-1 (il-1) levels and the hepatocyte histopathologicalfindings of rats’ induced with ccl4
publisher	Universitas Sultan Agung Semarang
publishDate	2020
url	https://doaj.org/article/d9910d37ce1e4299b60de3aac69cf608
work_keys_str_mv	AT caturretnolestari theeffectofcmcepropolisextractadministrationsoninterleukin1il1levelsandthehepatocytehistopathologicalfindingsofratsinducedwithccl4 AT titieksumarawati theeffectofcmcepropolisextractadministrationsoninterleukin1il1levelsandthehepatocytehistopathologicalfindingsofratsinducedwithccl4 AT taufiqurrachmannasihun theeffectofcmcepropolisextractadministrationsoninterleukin1il1levelsandthehepatocytehistopathologicalfindingsofratsinducedwithccl4 AT caturretnolestari effectofcmcepropolisextractadministrationsoninterleukin1il1levelsandthehepatocytehistopathologicalfindingsofratsinducedwithccl4 AT titieksumarawati effectofcmcepropolisextractadministrationsoninterleukin1il1levelsandthehepatocytehistopathologicalfindingsofratsinducedwithccl4 AT taufiqurrachmannasihun effectofcmcepropolisextractadministrationsoninterleukin1il1levelsandthehepatocytehistopathologicalfindingsofratsinducedwithccl4
_version_	1718413529292210176

The Effect of CMCE Propolis Extract Administrations on Interleukin-1 (IL-1) Levels and the Hepatocyte HistopathologicalFindings of Rats’ Induced with CCl4

Ejemplares similares