In situ tissue engineering with synthetic self-assembling peptide nanofiber scaffolds, PuraMatrix, for mucosal regeneration in the rat middle-ear

Naotaro Akiyama,1 Tomomi Yamamoto-Fukuda,1,2 Haruo Takahashi,1 Takehiko Koji21Department of Otolaryngology – Head and Neck Surgery, 2Department of Histology and Cell Biology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, JapanAbstract: Middle-ear mucosa maintains mi...

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Autores principales:	Akiyama N, Yamamoto-Fukuda T, Takahashi H, Koji T
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/d9948881fa004afc89647ca9fa997cf5
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spelling	oai:doaj.org-article:d9948881fa004afc89647ca9fa997cf52021-12-02T07:41:51ZIn situ tissue engineering with synthetic self-assembling peptide nanofiber scaffolds, PuraMatrix, for mucosal regeneration in the rat middle-ear1176-91141178-2013https://doaj.org/article/d9948881fa004afc89647ca9fa997cf52013-07-01T00:00:00Zhttp://www.dovepress.com/in-situ-tissue-engineering-with-synthetic-self-assembling-peptide-nano-a13770https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Naotaro Akiyama,1 Tomomi Yamamoto-Fukuda,1,2 Haruo Takahashi,1 Takehiko Koji21Department of Otolaryngology – Head and Neck Surgery, 2Department of Histology and Cell Biology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, JapanAbstract: Middle-ear mucosa maintains middle-ear pressure. However, the majority of surgical cases exhibit inadequate middle-ear mucosal regeneration, and mucosal transplantation is necessary in such cases. The aim of the present study was to assess the feasibility of transplantation of isolated mucosal cells encapsulated within synthetic self-assembling peptide nanofiber scaffolds using PuraMatrix, which has been successfully used as scaffolding in tissue engineering, for the repair of damaged middle ear. Middle-ear bullae with mucosa were removed from Sprague Dawley (SD) transgenic rats, transfected with enhanced green fluorescent protein (EGFP) transgene and excised into small pieces, then cultured up to the third passage. After surgical elimination of middle-ear mucosa in SD recipient rats, donor cells were encapsulated within PuraMatrix and transplanted into these immunosuppressed rats. Primary cultured cells were positive for pancytokeratin but not for vimentin, and retained the character of middle-ear epithelial cells. A high proportion of EGFP-expressing cells were found in the recipient middle ear after transplantation with PuraMatrix, but not without PuraMatrix. These cells retained normal morphology and function, as confirmed by histological examination, immunohistochemistry, and electron microscopy, and multiplied to form new epithelial and subepithelial layers together with basement membrane. The present study demonstrated the feasibility of transplantation of cultured middle-ear mucosal epithelial cells encapsulated within PuraMatrix for regeneration of surgically eliminated mucosa of the middle ear in SD rats.Keywords: nanofiber, synthetic self-assembling peptide scaffolds, regeneration, middle-ear mucosa, in situ tissue engineeringAkiyama NYamamoto-Fukuda TTakahashi HKoji TDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2013, Iss default, Pp 2629-2640 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Akiyama N Yamamoto-Fukuda T Takahashi H Koji T In situ tissue engineering with synthetic self-assembling peptide nanofiber scaffolds, PuraMatrix, for mucosal regeneration in the rat middle-ear
description	Naotaro Akiyama,1 Tomomi Yamamoto-Fukuda,1,2 Haruo Takahashi,1 Takehiko Koji21Department of Otolaryngology – Head and Neck Surgery, 2Department of Histology and Cell Biology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, JapanAbstract: Middle-ear mucosa maintains middle-ear pressure. However, the majority of surgical cases exhibit inadequate middle-ear mucosal regeneration, and mucosal transplantation is necessary in such cases. The aim of the present study was to assess the feasibility of transplantation of isolated mucosal cells encapsulated within synthetic self-assembling peptide nanofiber scaffolds using PuraMatrix, which has been successfully used as scaffolding in tissue engineering, for the repair of damaged middle ear. Middle-ear bullae with mucosa were removed from Sprague Dawley (SD) transgenic rats, transfected with enhanced green fluorescent protein (EGFP) transgene and excised into small pieces, then cultured up to the third passage. After surgical elimination of middle-ear mucosa in SD recipient rats, donor cells were encapsulated within PuraMatrix and transplanted into these immunosuppressed rats. Primary cultured cells were positive for pancytokeratin but not for vimentin, and retained the character of middle-ear epithelial cells. A high proportion of EGFP-expressing cells were found in the recipient middle ear after transplantation with PuraMatrix, but not without PuraMatrix. These cells retained normal morphology and function, as confirmed by histological examination, immunohistochemistry, and electron microscopy, and multiplied to form new epithelial and subepithelial layers together with basement membrane. The present study demonstrated the feasibility of transplantation of cultured middle-ear mucosal epithelial cells encapsulated within PuraMatrix for regeneration of surgically eliminated mucosa of the middle ear in SD rats.Keywords: nanofiber, synthetic self-assembling peptide scaffolds, regeneration, middle-ear mucosa, in situ tissue engineering
format	article
author	Akiyama N Yamamoto-Fukuda T Takahashi H Koji T
author_facet	Akiyama N Yamamoto-Fukuda T Takahashi H Koji T
author_sort	Akiyama N
title	In situ tissue engineering with synthetic self-assembling peptide nanofiber scaffolds, PuraMatrix, for mucosal regeneration in the rat middle-ear
title_short	In situ tissue engineering with synthetic self-assembling peptide nanofiber scaffolds, PuraMatrix, for mucosal regeneration in the rat middle-ear
title_full	In situ tissue engineering with synthetic self-assembling peptide nanofiber scaffolds, PuraMatrix, for mucosal regeneration in the rat middle-ear
title_fullStr	In situ tissue engineering with synthetic self-assembling peptide nanofiber scaffolds, PuraMatrix, for mucosal regeneration in the rat middle-ear
title_full_unstemmed	In situ tissue engineering with synthetic self-assembling peptide nanofiber scaffolds, PuraMatrix, for mucosal regeneration in the rat middle-ear
title_sort	in situ tissue engineering with synthetic self-assembling peptide nanofiber scaffolds, puramatrix, for mucosal regeneration in the rat middle-ear
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/d9948881fa004afc89647ca9fa997cf5
work_keys_str_mv	AT akiyaman insitutissueengineeringwithsyntheticselfassemblingpeptidenanofiberscaffoldspuramatrixformucosalregenerationintheratmiddleear AT yamamotofukudat insitutissueengineeringwithsyntheticselfassemblingpeptidenanofiberscaffoldspuramatrixformucosalregenerationintheratmiddleear AT takahashih insitutissueengineeringwithsyntheticselfassemblingpeptidenanofiberscaffoldspuramatrixformucosalregenerationintheratmiddleear AT kojit insitutissueengineeringwithsyntheticselfassemblingpeptidenanofiberscaffoldspuramatrixformucosalregenerationintheratmiddleear
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In situ tissue engineering with synthetic self-assembling peptide nanofiber scaffolds, PuraMatrix, for mucosal regeneration in the rat middle-ear

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