Sensitivity to tumor development by TALEN-mediated Trp53 mutant genes in the susceptible FVB/N mice and the resistance C57BL/6 mice

Abstract Background This study was undertaken to compare the sensitivities of mice strains during tumor induction by transcription activator-like effector nucleases (TALEN)-mediated Trp53 mutant gene. Alterations of their tumorigenic phenotypes including survival rate, tumor formation and tumor spec...

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Autores principales:	Woo Bin Yun, Ji Eun Kim, Mi Lim Lee, Jun Young Choi, Jin Ju Park, Bo Ram Song, Byeong Cheol Kang, Ki Taek Nam, Han-Woong Lee, Dae Youn Hwang
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Trp53 Tumorigenesis TALEN FVB/N C57BL/6 Medicine (General) R5-920 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/d9a0a12503cf4b28be4de1b7ca1a1dd9
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spelling	oai:doaj.org-article:d9a0a12503cf4b28be4de1b7ca1a1dd92021-12-05T12:10:42ZSensitivity to tumor development by TALEN-mediated Trp53 mutant genes in the susceptible FVB/N mice and the resistance C57BL/6 mice10.1186/s42826-021-00107-y2233-7660https://doaj.org/article/d9a0a12503cf4b28be4de1b7ca1a1dd92021-11-01T00:00:00Zhttps://doi.org/10.1186/s42826-021-00107-yhttps://doaj.org/toc/2233-7660Abstract Background This study was undertaken to compare the sensitivities of mice strains during tumor induction by transcription activator-like effector nucleases (TALEN)-mediated Trp53 mutant gene. Alterations of their tumorigenic phenotypes including survival rate, tumor formation and tumor spectrum, were assessed in FVB/N-Trp53em2Hwl/Korl and C57BL/6-Trp53em1Hwl/Korl knockout (KO) mice over 16 weeks. Results Most of the physiological phenotypes factors were observed to be higher in FVB/N-Trp53em2Hwl/Korl KO mice than C57BL/6-Trp53em1Hwl/Korl KO mice, although there were significant differences in the body weight, immune organ weight, number of red blood cells, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), platelet count (PLT), total bilirubin (Bil-T) and glucose (Glu) levels in the KO mice relative to the wild type (WT) mice. Furthermore, numerous solid tumors were also observed in various regions of the surface skin of FVB/N-Trp53em2Hwl/Korl KO mice, but were not detected in C57BL/6-Trp53em1Hwl/Korl KO mice. The most frequently observed tumor in both the Trp53 KO mice was malignant lymphoma, while soft tissue teratomas and hemangiosarcomas were only detected in the FVB/N-Trp53em2Hwl/Korl KO mice. Conclusions Our results indicate that the spectrum and incidence of tumors induced by the TALEN-mediated Trp53 mutant gene is greater in FVB/N-Trp53em2Hwl/Korl KO mice than C57BL/6-Trp53em1Hwl/Korl KO mice over 16 weeks.Woo Bin YunJi Eun KimMi Lim LeeJun Young ChoiJin Ju ParkBo Ram SongByeong Cheol KangKi Taek NamHan-Woong LeeDae Youn HwangBMCarticleTrp53TumorigenesisTALENFVB/NC57BL/6Medicine (General)R5-920Biology (General)QH301-705.5ENLaboratory Animal Research, Vol 37, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Trp53 Tumorigenesis TALEN FVB/N C57BL/6 Medicine (General) R5-920 Biology (General) QH301-705.5
spellingShingle	Trp53 Tumorigenesis TALEN FVB/N C57BL/6 Medicine (General) R5-920 Biology (General) QH301-705.5 Woo Bin Yun Ji Eun Kim Mi Lim Lee Jun Young Choi Jin Ju Park Bo Ram Song Byeong Cheol Kang Ki Taek Nam Han-Woong Lee Dae Youn Hwang Sensitivity to tumor development by TALEN-mediated Trp53 mutant genes in the susceptible FVB/N mice and the resistance C57BL/6 mice
description	Abstract Background This study was undertaken to compare the sensitivities of mice strains during tumor induction by transcription activator-like effector nucleases (TALEN)-mediated Trp53 mutant gene. Alterations of their tumorigenic phenotypes including survival rate, tumor formation and tumor spectrum, were assessed in FVB/N-Trp53em2Hwl/Korl and C57BL/6-Trp53em1Hwl/Korl knockout (KO) mice over 16 weeks. Results Most of the physiological phenotypes factors were observed to be higher in FVB/N-Trp53em2Hwl/Korl KO mice than C57BL/6-Trp53em1Hwl/Korl KO mice, although there were significant differences in the body weight, immune organ weight, number of red blood cells, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), platelet count (PLT), total bilirubin (Bil-T) and glucose (Glu) levels in the KO mice relative to the wild type (WT) mice. Furthermore, numerous solid tumors were also observed in various regions of the surface skin of FVB/N-Trp53em2Hwl/Korl KO mice, but were not detected in C57BL/6-Trp53em1Hwl/Korl KO mice. The most frequently observed tumor in both the Trp53 KO mice was malignant lymphoma, while soft tissue teratomas and hemangiosarcomas were only detected in the FVB/N-Trp53em2Hwl/Korl KO mice. Conclusions Our results indicate that the spectrum and incidence of tumors induced by the TALEN-mediated Trp53 mutant gene is greater in FVB/N-Trp53em2Hwl/Korl KO mice than C57BL/6-Trp53em1Hwl/Korl KO mice over 16 weeks.
format	article
author	Woo Bin Yun Ji Eun Kim Mi Lim Lee Jun Young Choi Jin Ju Park Bo Ram Song Byeong Cheol Kang Ki Taek Nam Han-Woong Lee Dae Youn Hwang
author_facet	Woo Bin Yun Ji Eun Kim Mi Lim Lee Jun Young Choi Jin Ju Park Bo Ram Song Byeong Cheol Kang Ki Taek Nam Han-Woong Lee Dae Youn Hwang
author_sort	Woo Bin Yun
title	Sensitivity to tumor development by TALEN-mediated Trp53 mutant genes in the susceptible FVB/N mice and the resistance C57BL/6 mice
title_short	Sensitivity to tumor development by TALEN-mediated Trp53 mutant genes in the susceptible FVB/N mice and the resistance C57BL/6 mice
title_full	Sensitivity to tumor development by TALEN-mediated Trp53 mutant genes in the susceptible FVB/N mice and the resistance C57BL/6 mice
title_fullStr	Sensitivity to tumor development by TALEN-mediated Trp53 mutant genes in the susceptible FVB/N mice and the resistance C57BL/6 mice
title_full_unstemmed	Sensitivity to tumor development by TALEN-mediated Trp53 mutant genes in the susceptible FVB/N mice and the resistance C57BL/6 mice
title_sort	sensitivity to tumor development by talen-mediated trp53 mutant genes in the susceptible fvb/n mice and the resistance c57bl/6 mice
publisher	BMC
publishDate	2021
url	https://doaj.org/article/d9a0a12503cf4b28be4de1b7ca1a1dd9
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Sensitivity to tumor development by TALEN-mediated Trp53 mutant genes in the susceptible FVB/N mice and the resistance C57BL/6 mice

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