Evolution of a key enzyme of aerobic metabolism reveals Proterozoic functional subunit duplication events and an ancient origin of animals

Abstract The biological toolkits for aerobic respiration were critical for the rise and diversification of early animals. Aerobic life forms generate ATP through the oxidation of organic molecules in a process known as Krebs’ Cycle, where the enzyme isocitrate dehydrogenase (IDH) regulates the cycle...

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Autores principales:	Bruno Santos Bezerra, Flavia Ariany Belato, Beatriz Mello, Federico Brown, Christopher J. Coates, Juliana de Moraes Leme, Ricardo I. F. Trindade, Elisa Maria Costa-Paiva
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d9a4072476a8469a8bb2c1a733d72d69
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spelling	oai:doaj.org-article:d9a4072476a8469a8bb2c1a733d72d692021-12-02T14:53:35ZEvolution of a key enzyme of aerobic metabolism reveals Proterozoic functional subunit duplication events and an ancient origin of animals10.1038/s41598-021-95094-42045-2322https://doaj.org/article/d9a4072476a8469a8bb2c1a733d72d692021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95094-4https://doaj.org/toc/2045-2322Abstract The biological toolkits for aerobic respiration were critical for the rise and diversification of early animals. Aerobic life forms generate ATP through the oxidation of organic molecules in a process known as Krebs’ Cycle, where the enzyme isocitrate dehydrogenase (IDH) regulates the cycle's turnover rate. Evolutionary reconstructions and molecular dating of proteins related to oxidative metabolism, such as IDH, can therefore provide an estimate of when the diversification of major taxa occurred, and their coevolution with the oxidative state of oceans and atmosphere. To establish the evolutionary history and divergence time of NAD-dependent IDH, we examined transcriptomic data from 195 eukaryotes (mostly animals). We demonstrate that two duplication events occurred in the evolutionary history of NAD-IDH, one in the ancestor of eukaryotes approximately at 1967 Ma, and another at 1629 Ma, both in the Paleoproterozoic Era. Moreover, NAD-IDH regulatory subunits β and γ are exclusive to metazoans, arising in the Mesoproterozoic. Our results therefore support the concept of an ‘‘earlier-than-Tonian’’ diversification of eukaryotes and the pre-Cryogenian emergence of a metazoan IDH enzyme.Bruno Santos BezerraFlavia Ariany BelatoBeatriz MelloFederico BrownChristopher J. CoatesJuliana de Moraes LemeRicardo I. F. TrindadeElisa Maria Costa-PaivaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Bruno Santos Bezerra Flavia Ariany Belato Beatriz Mello Federico Brown Christopher J. Coates Juliana de Moraes Leme Ricardo I. F. Trindade Elisa Maria Costa-Paiva Evolution of a key enzyme of aerobic metabolism reveals Proterozoic functional subunit duplication events and an ancient origin of animals
description	Abstract The biological toolkits for aerobic respiration were critical for the rise and diversification of early animals. Aerobic life forms generate ATP through the oxidation of organic molecules in a process known as Krebs’ Cycle, where the enzyme isocitrate dehydrogenase (IDH) regulates the cycle's turnover rate. Evolutionary reconstructions and molecular dating of proteins related to oxidative metabolism, such as IDH, can therefore provide an estimate of when the diversification of major taxa occurred, and their coevolution with the oxidative state of oceans and atmosphere. To establish the evolutionary history and divergence time of NAD-dependent IDH, we examined transcriptomic data from 195 eukaryotes (mostly animals). We demonstrate that two duplication events occurred in the evolutionary history of NAD-IDH, one in the ancestor of eukaryotes approximately at 1967 Ma, and another at 1629 Ma, both in the Paleoproterozoic Era. Moreover, NAD-IDH regulatory subunits β and γ are exclusive to metazoans, arising in the Mesoproterozoic. Our results therefore support the concept of an ‘‘earlier-than-Tonian’’ diversification of eukaryotes and the pre-Cryogenian emergence of a metazoan IDH enzyme.
format	article
author	Bruno Santos Bezerra Flavia Ariany Belato Beatriz Mello Federico Brown Christopher J. Coates Juliana de Moraes Leme Ricardo I. F. Trindade Elisa Maria Costa-Paiva
author_facet	Bruno Santos Bezerra Flavia Ariany Belato Beatriz Mello Federico Brown Christopher J. Coates Juliana de Moraes Leme Ricardo I. F. Trindade Elisa Maria Costa-Paiva
author_sort	Bruno Santos Bezerra
title	Evolution of a key enzyme of aerobic metabolism reveals Proterozoic functional subunit duplication events and an ancient origin of animals
title_short	Evolution of a key enzyme of aerobic metabolism reveals Proterozoic functional subunit duplication events and an ancient origin of animals
title_full	Evolution of a key enzyme of aerobic metabolism reveals Proterozoic functional subunit duplication events and an ancient origin of animals
title_fullStr	Evolution of a key enzyme of aerobic metabolism reveals Proterozoic functional subunit duplication events and an ancient origin of animals
title_full_unstemmed	Evolution of a key enzyme of aerobic metabolism reveals Proterozoic functional subunit duplication events and an ancient origin of animals
title_sort	evolution of a key enzyme of aerobic metabolism reveals proterozoic functional subunit duplication events and an ancient origin of animals
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d9a4072476a8469a8bb2c1a733d72d69
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Evolution of a key enzyme of aerobic metabolism reveals Proterozoic functional subunit duplication events and an ancient origin of animals

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