Detecting a psoriatic antigen analogous to infectious prion proteins

Until now, psoriatic antigen as a specific antigen derived from some infectious agent potentially related to origin of psoriasis has not been identified, thereby strongly arguing against infectious theory of psoriasis. However, the lack of specific psoriasis-associated antigens may be theoretically...

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Autor principal:	B. F. Sinitsyn
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2019
Materias:	psoriasis squamous elements acidin-pepsin peptic hydrolysis psoriatic antigen infectious prion proteins prpsc prpsc analogue Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/d9ab47cf3d1a4d9c8247076b047fe8fd
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spelling	oai:doaj.org-article:d9ab47cf3d1a4d9c8247076b047fe8fd2021-11-22T07:09:53ZDetecting a psoriatic antigen analogous to infectious prion proteins2220-76192313-739810.15789/2220-7619-2019-3-4-589-594https://doaj.org/article/d9ab47cf3d1a4d9c8247076b047fe8fd2019-11-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/686https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398Until now, psoriatic antigen as a specific antigen derived from some infectious agent potentially related to origin of psoriasis has not been identified, thereby strongly arguing against infectious theory of psoriasis. However, the lack of specific psoriasis-associated antigens may be theoretically accounted for by an idea that psoriatic antigen could be analogous to infectious prion proteins (PrPSc analogue). It might be identical to some epidermal protein in psoriasis-free subjects that might differ antigenically from related normal protein by resistance to digestive enzymes similarly to PrPSc. Since PrPSc cytopathogenic effect is associated with its location within cell structures of affected body tissue, by analogy with PrPSc we aimed at identifying a potential carrier of psoriatic antigen by examining peptic hydrolysates of psoriatic squamous elements. Psoriatic squamous elements and epidermal stratum corneum were collected from heel area of healthy subjects with a metal grater for further examination. Squamous elements were homogenized in isotonic saline followed by centrifugation to separate them from soluble components. Homogenates of squamous elements structures and their homogenate supernatants underwent peptic hydrolysis reaction. Antigens and their identity were analysed by Ouchterlony immunoprecipitation in agar with antiserum obtained after immunizing rabbits with homogenate of the psoriatic squamous element structures, before and after hydrolysis, using peptic hydrolysates of squamous element structures and relevant supernatants. It was found that two antigens were precipitated in peptic hydrolysates of homogenates of psoriatic squamous cell structures, underlying acidin-pepsin resistance of antigen carriers. However, compared to the remainder of antigens precipitated in examined substrates only one out of two antigens might be considered as a psoriatic antigen being some PrPSc analogue that was identical to one of supernatant antigens derived from epidermal stratum corneum homogenates collected from healthy subjects. Similar to PrPSc, it differed from antigenically related normal protein by acidin-pepsin resistance. Albeit confirming available reports revealing no psoriasis-associated pathogen-specific antigens, this, nonetheless, might not be sufficient for rejecting infectious theory of psoriasis, as psoriatic antigen exhibits some properties similar to those of PrPSc.B. F. SinitsynSankt-Peterburg : NIIÈM imeni Pasteraarticlepsoriasissquamous elementsacidin-pepsinpeptic hydrolysispsoriatic antigeninfectious prion proteinsprpscprpsc analogueInfectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 9, Iss 3-4, Pp 589-594 (2019)
institution	DOAJ
collection	DOAJ
language	RU
topic	psoriasis squamous elements acidin-pepsin peptic hydrolysis psoriatic antigen infectious prion proteins prpsc prpsc analogue Infectious and parasitic diseases RC109-216
spellingShingle	psoriasis squamous elements acidin-pepsin peptic hydrolysis psoriatic antigen infectious prion proteins prpsc prpsc analogue Infectious and parasitic diseases RC109-216 B. F. Sinitsyn Detecting a psoriatic antigen analogous to infectious prion proteins
description	Until now, psoriatic antigen as a specific antigen derived from some infectious agent potentially related to origin of psoriasis has not been identified, thereby strongly arguing against infectious theory of psoriasis. However, the lack of specific psoriasis-associated antigens may be theoretically accounted for by an idea that psoriatic antigen could be analogous to infectious prion proteins (PrPSc analogue). It might be identical to some epidermal protein in psoriasis-free subjects that might differ antigenically from related normal protein by resistance to digestive enzymes similarly to PrPSc. Since PrPSc cytopathogenic effect is associated with its location within cell structures of affected body tissue, by analogy with PrPSc we aimed at identifying a potential carrier of psoriatic antigen by examining peptic hydrolysates of psoriatic squamous elements. Psoriatic squamous elements and epidermal stratum corneum were collected from heel area of healthy subjects with a metal grater for further examination. Squamous elements were homogenized in isotonic saline followed by centrifugation to separate them from soluble components. Homogenates of squamous elements structures and their homogenate supernatants underwent peptic hydrolysis reaction. Antigens and their identity were analysed by Ouchterlony immunoprecipitation in agar with antiserum obtained after immunizing rabbits with homogenate of the psoriatic squamous element structures, before and after hydrolysis, using peptic hydrolysates of squamous element structures and relevant supernatants. It was found that two antigens were precipitated in peptic hydrolysates of homogenates of psoriatic squamous cell structures, underlying acidin-pepsin resistance of antigen carriers. However, compared to the remainder of antigens precipitated in examined substrates only one out of two antigens might be considered as a psoriatic antigen being some PrPSc analogue that was identical to one of supernatant antigens derived from epidermal stratum corneum homogenates collected from healthy subjects. Similar to PrPSc, it differed from antigenically related normal protein by acidin-pepsin resistance. Albeit confirming available reports revealing no psoriasis-associated pathogen-specific antigens, this, nonetheless, might not be sufficient for rejecting infectious theory of psoriasis, as psoriatic antigen exhibits some properties similar to those of PrPSc.
format	article
author	B. F. Sinitsyn
author_facet	B. F. Sinitsyn
author_sort	B. F. Sinitsyn
title	Detecting a psoriatic antigen analogous to infectious prion proteins
title_short	Detecting a psoriatic antigen analogous to infectious prion proteins
title_full	Detecting a psoriatic antigen analogous to infectious prion proteins
title_fullStr	Detecting a psoriatic antigen analogous to infectious prion proteins
title_full_unstemmed	Detecting a psoriatic antigen analogous to infectious prion proteins
title_sort	detecting a psoriatic antigen analogous to infectious prion proteins
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2019
url	https://doaj.org/article/d9ab47cf3d1a4d9c8247076b047fe8fd
work_keys_str_mv	AT bfsinitsyn detectingapsoriaticantigenanalogoustoinfectiousprionproteins
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Detecting a psoriatic antigen analogous to infectious prion proteins

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