Engraftment of donor mesenchymal stem cells in chimeric BXSB includes vascular endothelial cells and hepatocytes

Olcay Y Jones1, Faysal Gok2, Elisabeth J Rushing3, Iren Horkayne-Szakaly4, Atif A Ahmed51Department of Pediatrics, Walter Reed National Military Medical Center, Bethesda, MD, USA; 2Department of Pediatrics, Gulhane Military Medical Academy, Ankara, Turkey; 3Institut f&uuml;r Neuropathologie,...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jones OY, Gok F, Rushing EJ, Horkayne-Szakaly I, Ahmed AA
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Cytology QH573-671
Acceso en línea:	https://doaj.org/article/d9c1d46c3eb446a4b37d064bd356ffa3
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d9c1d46c3eb446a4b37d064bd356ffa3
record_format	dspace
spelling	oai:doaj.org-article:d9c1d46c3eb446a4b37d064bd356ffa32021-12-02T02:48:26ZEngraftment of donor mesenchymal stem cells in chimeric BXSB includes vascular endothelial cells and hepatocytes1178-6957https://doaj.org/article/d9c1d46c3eb446a4b37d064bd356ffa32011-12-01T00:00:00Zhttp://www.dovepress.com/engraftment-of-donor-mesenchymal-stem-cells-in-chimeric-bxsb-includes--a8839https://doaj.org/toc/1178-6957Olcay Y Jones1, Faysal Gok2, Elisabeth J Rushing3, Iren Horkayne-Szakaly4, Atif A Ahmed51Department of Pediatrics, Walter Reed National Military Medical Center, Bethesda, MD, USA; 2Department of Pediatrics, Gulhane Military Medical Academy, Ankara, Turkey; 3Institut f&uuml;r Neuropathologie, Universit&auml;ts Spital Z&uuml;rich, Z&uuml;rich, Switzerland; 4Department of Neuropathology, Armed Forces Institute of Pathology, Washington, DC, USA; 5Department of Pathology and Laboratory Medicine, Children&#39;s Mercy Hospitals and Clinics, Kansas City, MO, USAAbstract: Somatic tissue engraftment was studied in BXSB mice treated with mesenchymal stem cell transplantation. Hosts were conditioned with nonlethal radiation prior to introducing donor cells from major histocompatibility complex-matched green fluorescent protein transgenic mice. Transplant protocols differed for route of injection, ie, intravenous (i.v.) versus intraperitoneal (i.p.), and source of mesenchymal stem cells, ie, unfractionated bone marrow cells, ex vivo expanded mesenchymal stem cells, or bone chips. Tissue chimerism was determined after short (10&ndash;12 weeks) or long (62 weeks) posttransplant follow-up by immunohistochemistry for green fluorescent protein. Engraftment of endothelial cells was seen in several organs including liver sinusoidal cells in i.v. treated mice with ex vivo expanded mesenchymal stem cells or with unfractionated bone marrow cells. Periportal engraftment of liver hepatocytes, but not engraftment of endothelial cells, was found in mice injected i.p. with bone chips. Engraftment of adipocytes was a common denominator in both i.v. and i.p. routes and occurred during early phases post-transplant. Disease control was more robust in mice that received both i.v. bone marrow and i.p. bone chips compared to mice that received i.v. bone marrow alone. Thus, the data support potential use of mesenchymal stem cell transplant for treatment of severe lupus. Future studies are needed to optimize transplant conditions and tailor protocols that may in part be guided by fat and endothelial biomarkers. Furthermore, the role of liver chimerism in disease control and the nature of cellular communication among donor hematopoietic and mesenchymal stem cells in a chimeric host merit further investigation.Keywords: lupus, mice, mesenchymal stem cells, endothelial cells, liverJones OYGok FRushing EJHorkayne-Szakaly IAhmed AADove Medical PressarticleCytologyQH573-671ENStem Cells and Cloning: Advances and Applications, Vol 2011, Iss default, Pp 73-78 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cytology QH573-671
spellingShingle	Cytology QH573-671 Jones OY Gok F Rushing EJ Horkayne-Szakaly I Ahmed AA Engraftment of donor mesenchymal stem cells in chimeric BXSB includes vascular endothelial cells and hepatocytes
description	Olcay Y Jones1, Faysal Gok2, Elisabeth J Rushing3, Iren Horkayne-Szakaly4, Atif A Ahmed51Department of Pediatrics, Walter Reed National Military Medical Center, Bethesda, MD, USA; 2Department of Pediatrics, Gulhane Military Medical Academy, Ankara, Turkey; 3Institut f&uuml;r Neuropathologie, Universit&auml;ts Spital Z&uuml;rich, Z&uuml;rich, Switzerland; 4Department of Neuropathology, Armed Forces Institute of Pathology, Washington, DC, USA; 5Department of Pathology and Laboratory Medicine, Children&#39;s Mercy Hospitals and Clinics, Kansas City, MO, USAAbstract: Somatic tissue engraftment was studied in BXSB mice treated with mesenchymal stem cell transplantation. Hosts were conditioned with nonlethal radiation prior to introducing donor cells from major histocompatibility complex-matched green fluorescent protein transgenic mice. Transplant protocols differed for route of injection, ie, intravenous (i.v.) versus intraperitoneal (i.p.), and source of mesenchymal stem cells, ie, unfractionated bone marrow cells, ex vivo expanded mesenchymal stem cells, or bone chips. Tissue chimerism was determined after short (10&ndash;12 weeks) or long (62 weeks) posttransplant follow-up by immunohistochemistry for green fluorescent protein. Engraftment of endothelial cells was seen in several organs including liver sinusoidal cells in i.v. treated mice with ex vivo expanded mesenchymal stem cells or with unfractionated bone marrow cells. Periportal engraftment of liver hepatocytes, but not engraftment of endothelial cells, was found in mice injected i.p. with bone chips. Engraftment of adipocytes was a common denominator in both i.v. and i.p. routes and occurred during early phases post-transplant. Disease control was more robust in mice that received both i.v. bone marrow and i.p. bone chips compared to mice that received i.v. bone marrow alone. Thus, the data support potential use of mesenchymal stem cell transplant for treatment of severe lupus. Future studies are needed to optimize transplant conditions and tailor protocols that may in part be guided by fat and endothelial biomarkers. Furthermore, the role of liver chimerism in disease control and the nature of cellular communication among donor hematopoietic and mesenchymal stem cells in a chimeric host merit further investigation.Keywords: lupus, mice, mesenchymal stem cells, endothelial cells, liver
format	article
author	Jones OY Gok F Rushing EJ Horkayne-Szakaly I Ahmed AA
author_facet	Jones OY Gok F Rushing EJ Horkayne-Szakaly I Ahmed AA
author_sort	Jones OY
title	Engraftment of donor mesenchymal stem cells in chimeric BXSB includes vascular endothelial cells and hepatocytes
title_short	Engraftment of donor mesenchymal stem cells in chimeric BXSB includes vascular endothelial cells and hepatocytes
title_full	Engraftment of donor mesenchymal stem cells in chimeric BXSB includes vascular endothelial cells and hepatocytes
title_fullStr	Engraftment of donor mesenchymal stem cells in chimeric BXSB includes vascular endothelial cells and hepatocytes
title_full_unstemmed	Engraftment of donor mesenchymal stem cells in chimeric BXSB includes vascular endothelial cells and hepatocytes
title_sort	engraftment of donor mesenchymal stem cells in chimeric bxsb includes vascular endothelial cells and hepatocytes
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/d9c1d46c3eb446a4b37d064bd356ffa3
work_keys_str_mv	AT jonesoy engraftmentofdonormesenchymalstemcellsinchimericbxsbincludesvascularendothelialcellsandhepatocytes AT gokf engraftmentofdonormesenchymalstemcellsinchimericbxsbincludesvascularendothelialcellsandhepatocytes AT rushingej engraftmentofdonormesenchymalstemcellsinchimericbxsbincludesvascularendothelialcellsandhepatocytes AT horkayneszakalyi engraftmentofdonormesenchymalstemcellsinchimericbxsbincludesvascularendothelialcellsandhepatocytes AT ahmedaa engraftmentofdonormesenchymalstemcellsinchimericbxsbincludesvascularendothelialcellsandhepatocytes
_version_	1718402191321989120

Engraftment of donor mesenchymal stem cells in chimeric BXSB includes vascular endothelial cells and hepatocytes

Ejemplares similares