Cocaine modulates the expression of opioid receptors and miR-let-7d in zebrafish embryos.

Prenatal exposure to cocaine, in mammals, has been shown to interfere with the expression of opioid receptors, which can have repercussions in its activity. Likewise, microRNAs, such as let-7, have been shown to regulate the expression of opioid receptors and hence their functions in mammals and in...

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Autores principales:	Roger López-Bellido, Katherine Barreto-Valer, Fátima Macho Sánchez-Simón, Raquel E Rodríguez
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d9cbd49b54be425ba980f5337b6d16ef
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spelling	oai:doaj.org-article:d9cbd49b54be425ba980f5337b6d16ef2021-11-18T08:06:39ZCocaine modulates the expression of opioid receptors and miR-let-7d in zebrafish embryos.1932-620310.1371/journal.pone.0050885https://doaj.org/article/d9cbd49b54be425ba980f5337b6d16ef2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23226419/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Prenatal exposure to cocaine, in mammals, has been shown to interfere with the expression of opioid receptors, which can have repercussions in its activity. Likewise, microRNAs, such as let-7, have been shown to regulate the expression of opioid receptors and hence their functions in mammals and in vitro experiments. In light of this, using the zebrafish embryos as a model our aim here was to evaluate the actions of cocaine in the expression of opioid receptors and let-7d miRNA during embryogenesis. In order to determine the effects produced by cocaine on the opioid receptors (zfmor, zfdor1 and zfdor2) and let-7d miRNA (dre-let-7d) and its precursors (dre-let-7d-1 and dre-let-7d-2), embryos were exposed to 1.5 µM cocaine hydrochloride (HCl). Our results revealed that cocaine upregulated dre-let-7d and its precursors, and also increased the expression of zfmor, zfdor1 and zfdor2 during early developmental stages and decreased them in late embryonic stages. The changes observed in the expression of opioid receptors might occur through dre-let-7d, since DNA sequences and the morpholinos of opioid receptors microinjections altered the expression of dre-let-7d and its precursors. Likewise, opioid receptors and dre-let-7d showed similar distributions in the central nervous system (CNS) and at the periphery, pointing to a possible interrelationship between them.In conclusion, the silencing and overexpression of opioid receptors altered the expression of dre-let-7d, which points to the notion that cocaine via dre-let-7 can modulate the expression of opioid receptors. Our study provides new insights into the actions of cocaine during zebrafish embryogenesis, indicating a role of miRNAs, let-7d, in development and its relationship with gene expression of opioid receptors, related to pain and addiction process.Roger López-BellidoKatherine Barreto-ValerFátima Macho Sánchez-SimónRaquel E RodríguezPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 11, p e50885 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Roger López-Bellido Katherine Barreto-Valer Fátima Macho Sánchez-Simón Raquel E Rodríguez Cocaine modulates the expression of opioid receptors and miR-let-7d in zebrafish embryos.
description	Prenatal exposure to cocaine, in mammals, has been shown to interfere with the expression of opioid receptors, which can have repercussions in its activity. Likewise, microRNAs, such as let-7, have been shown to regulate the expression of opioid receptors and hence their functions in mammals and in vitro experiments. In light of this, using the zebrafish embryos as a model our aim here was to evaluate the actions of cocaine in the expression of opioid receptors and let-7d miRNA during embryogenesis. In order to determine the effects produced by cocaine on the opioid receptors (zfmor, zfdor1 and zfdor2) and let-7d miRNA (dre-let-7d) and its precursors (dre-let-7d-1 and dre-let-7d-2), embryos were exposed to 1.5 µM cocaine hydrochloride (HCl). Our results revealed that cocaine upregulated dre-let-7d and its precursors, and also increased the expression of zfmor, zfdor1 and zfdor2 during early developmental stages and decreased them in late embryonic stages. The changes observed in the expression of opioid receptors might occur through dre-let-7d, since DNA sequences and the morpholinos of opioid receptors microinjections altered the expression of dre-let-7d and its precursors. Likewise, opioid receptors and dre-let-7d showed similar distributions in the central nervous system (CNS) and at the periphery, pointing to a possible interrelationship between them.In conclusion, the silencing and overexpression of opioid receptors altered the expression of dre-let-7d, which points to the notion that cocaine via dre-let-7 can modulate the expression of opioid receptors. Our study provides new insights into the actions of cocaine during zebrafish embryogenesis, indicating a role of miRNAs, let-7d, in development and its relationship with gene expression of opioid receptors, related to pain and addiction process.
format	article
author	Roger López-Bellido Katherine Barreto-Valer Fátima Macho Sánchez-Simón Raquel E Rodríguez
author_facet	Roger López-Bellido Katherine Barreto-Valer Fátima Macho Sánchez-Simón Raquel E Rodríguez
author_sort	Roger López-Bellido
title	Cocaine modulates the expression of opioid receptors and miR-let-7d in zebrafish embryos.
title_short	Cocaine modulates the expression of opioid receptors and miR-let-7d in zebrafish embryos.
title_full	Cocaine modulates the expression of opioid receptors and miR-let-7d in zebrafish embryos.
title_fullStr	Cocaine modulates the expression of opioid receptors and miR-let-7d in zebrafish embryos.
title_full_unstemmed	Cocaine modulates the expression of opioid receptors and miR-let-7d in zebrafish embryos.
title_sort	cocaine modulates the expression of opioid receptors and mir-let-7d in zebrafish embryos.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/d9cbd49b54be425ba980f5337b6d16ef
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Cocaine modulates the expression of opioid receptors and miR-let-7d in zebrafish embryos.

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