Riemerella anatipestifer AS87_RS09170 gene is responsible for biotin synthesis, bacterial morphology and virulence

Abstract Riemerella anatipestifer is a bacterial pathogen responsible for major economic losses within the duck industry. Recent studies have revealed that biotin biosynthesis is critical for the bacterium’s survival and virulence. We previously found that R. anatipestifer AS87_RS09170, a putative b...

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Autores principales:	Xiaomei Ren, Xiaolan Wang, Huoying Shi, Xuemei Zhang, Zongchao Chen, Kanwar Kumar Malhi, Chan Ding, Shengqing Yu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Anatipestifer RS09170 Gene Biotin Synthesis Riemerella Anatipestifer Biotin Biosynthesis Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d9cde892ec784d48876a87eb147f5533
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spelling	oai:doaj.org-article:d9cde892ec784d48876a87eb147f55332021-12-02T11:41:15ZRiemerella anatipestifer AS87_RS09170 gene is responsible for biotin synthesis, bacterial morphology and virulence10.1038/s41598-018-32905-12045-2322https://doaj.org/article/d9cde892ec784d48876a87eb147f55332018-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-32905-1https://doaj.org/toc/2045-2322Abstract Riemerella anatipestifer is a bacterial pathogen responsible for major economic losses within the duck industry. Recent studies have revealed that biotin biosynthesis is critical for the bacterium’s survival and virulence. We previously found that R. anatipestifer AS87_RS09170, a putative bioF gene, is important for bacterial virulence. In the present study, we characterized the AS87_RS09170 gene in R. anatipestifer strain Yb2. Sequence analysis indicated that the AS87_RS09170 gene is highly conserved among R. anatipestifer strains; the deduced protein harbored the conserved pyridoxal 5′-phosphate binding pocket of 8-amino-7-oxononanoate synthase. Western blot analysis demonstrated that the biotin-dependent enzyme was present in smaller quantities in the mutant strain Yb2ΔbioF compared to that of the wide-type strain Yb2, suggesting that the biotin biosynthesis was defective. The mutant strain Yb2ΔbioF displayed a decreased growth rate at the exponential phase in tryptic soy broth culture and in BeaverBeads Streptavidin treated tryptic soy broth culture, but recovered when biotin was supplemented. In addition, the mutant strain Yb2ΔbioF showed an enhanced biofilm formation, as well as increased adhesion and invasion capacities to duck embryo fibroblasts. Moreover, the mutant strain Yb2ΔbioF exhibited irregular shapes with budding vegetations and relatively thickened cell walls under scanning and transmission electron microscope observation, as well as a reduced capacity to establish systemic infection in a duck infection model. These results provide the first evidence that the R. anatipestifer AS87_RS09170 gene is responsible for biotin synthesis, bacterial morphology and virulence.Xiaomei RenXiaolan WangHuoying ShiXuemei ZhangZongchao ChenKanwar Kumar MalhiChan DingShengqing YuNature PortfolioarticleAnatipestiferRS09170 GeneBiotin SynthesisRiemerella AnatipestiferBiotin BiosynthesisMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-13 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Anatipestifer RS09170 Gene Biotin Synthesis Riemerella Anatipestifer Biotin Biosynthesis Medicine R Science Q
spellingShingle	Anatipestifer RS09170 Gene Biotin Synthesis Riemerella Anatipestifer Biotin Biosynthesis Medicine R Science Q Xiaomei Ren Xiaolan Wang Huoying Shi Xuemei Zhang Zongchao Chen Kanwar Kumar Malhi Chan Ding Shengqing Yu Riemerella anatipestifer AS87_RS09170 gene is responsible for biotin synthesis, bacterial morphology and virulence
description	Abstract Riemerella anatipestifer is a bacterial pathogen responsible for major economic losses within the duck industry. Recent studies have revealed that biotin biosynthesis is critical for the bacterium’s survival and virulence. We previously found that R. anatipestifer AS87_RS09170, a putative bioF gene, is important for bacterial virulence. In the present study, we characterized the AS87_RS09170 gene in R. anatipestifer strain Yb2. Sequence analysis indicated that the AS87_RS09170 gene is highly conserved among R. anatipestifer strains; the deduced protein harbored the conserved pyridoxal 5′-phosphate binding pocket of 8-amino-7-oxononanoate synthase. Western blot analysis demonstrated that the biotin-dependent enzyme was present in smaller quantities in the mutant strain Yb2ΔbioF compared to that of the wide-type strain Yb2, suggesting that the biotin biosynthesis was defective. The mutant strain Yb2ΔbioF displayed a decreased growth rate at the exponential phase in tryptic soy broth culture and in BeaverBeads Streptavidin treated tryptic soy broth culture, but recovered when biotin was supplemented. In addition, the mutant strain Yb2ΔbioF showed an enhanced biofilm formation, as well as increased adhesion and invasion capacities to duck embryo fibroblasts. Moreover, the mutant strain Yb2ΔbioF exhibited irregular shapes with budding vegetations and relatively thickened cell walls under scanning and transmission electron microscope observation, as well as a reduced capacity to establish systemic infection in a duck infection model. These results provide the first evidence that the R. anatipestifer AS87_RS09170 gene is responsible for biotin synthesis, bacterial morphology and virulence.
format	article
author	Xiaomei Ren Xiaolan Wang Huoying Shi Xuemei Zhang Zongchao Chen Kanwar Kumar Malhi Chan Ding Shengqing Yu
author_facet	Xiaomei Ren Xiaolan Wang Huoying Shi Xuemei Zhang Zongchao Chen Kanwar Kumar Malhi Chan Ding Shengqing Yu
author_sort	Xiaomei Ren
title	Riemerella anatipestifer AS87_RS09170 gene is responsible for biotin synthesis, bacterial morphology and virulence
title_short	Riemerella anatipestifer AS87_RS09170 gene is responsible for biotin synthesis, bacterial morphology and virulence
title_full	Riemerella anatipestifer AS87_RS09170 gene is responsible for biotin synthesis, bacterial morphology and virulence
title_fullStr	Riemerella anatipestifer AS87_RS09170 gene is responsible for biotin synthesis, bacterial morphology and virulence
title_full_unstemmed	Riemerella anatipestifer AS87_RS09170 gene is responsible for biotin synthesis, bacterial morphology and virulence
title_sort	riemerella anatipestifer as87_rs09170 gene is responsible for biotin synthesis, bacterial morphology and virulence
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/d9cde892ec784d48876a87eb147f5533
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Riemerella anatipestifer AS87_RS09170 gene is responsible for biotin synthesis, bacterial morphology and virulence

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