Examining the Possibility of Detecting Brucella Canis from Tissue Samples Using Bruce-Ladder Multiplex PCR Assay

The goal of this study was to compare the results of serological and conventional bacteriological methods with the results obtained using multiplex PCR Bruce-ladder assay. Based on the obtained results, the usability of the assay was assessed in regard to rapid diagnosis of canine brucellosis direct...

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Autores principales:	Stević Nataša, Mišić Dušan, Bogunović Danica, Matović Kazimir, Valčić Miroslav, Milovanović Milovan, Radojičić Sonja
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2017
Materias:	brucella canis bruce-ladder dogs reproductive organs Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/d9cfd33c0aef4b6989fcbd6de8423bb7
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spelling	oai:doaj.org-article:d9cfd33c0aef4b6989fcbd6de8423bb72021-11-17T21:27:52ZExamining the Possibility of Detecting Brucella Canis from Tissue Samples Using Bruce-Ladder Multiplex PCR Assay1820-744810.1515/acve-2017-0046https://doaj.org/article/d9cfd33c0aef4b6989fcbd6de8423bb72017-12-01T00:00:00Zhttps://doi.org/10.1515/acve-2017-0046https://doaj.org/toc/1820-7448The goal of this study was to compare the results of serological and conventional bacteriological methods with the results obtained using multiplex PCR Bruce-ladder assay. Based on the obtained results, the usability of the assay was assessed in regard to rapid diagnosis of canine brucellosis directly from the samples of reproductive organs of infected dogs. Out of 225 blood samples, 33 (14.67%) had a positive agglutination reaction. In this study, out of the 225 assayed reproductive organs of dogs, B. canis was isolated from 3 samples (1.33%), while the PCR Bruce-ladder assay detected two positive samples (0.88%). Two dogs from which B. canis was isolated, an antibody titer of 1/200 was established in blood serums, and third dog from which B. canis was isolated was negative using the tube agglutination test. From a total of 225 assayed organ samples, a positive PCR reaction was obtained from two samples. The obtained results show that the tube agglutination method remains the first choice for the detection of dogs infected with B. canis. In addition, whenever possible, it is necessary to try isolation. It is desirable to attempt the detection of B. canis in tissues using PCR, but the results may not be treated as definitive and reliable.Stević NatašaMišić DušanBogunović DanicaMatović KazimirValčić MiroslavMilovanović MilovanRadojičić SonjaSciendoarticlebrucella canisbruce-ladderdogsreproductive organsVeterinary medicineSF600-1100ENActa Veterinaria, Vol 67, Iss 4, Pp 551-561 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	brucella canis bruce-ladder dogs reproductive organs Veterinary medicine SF600-1100
spellingShingle	brucella canis bruce-ladder dogs reproductive organs Veterinary medicine SF600-1100 Stević Nataša Mišić Dušan Bogunović Danica Matović Kazimir Valčić Miroslav Milovanović Milovan Radojičić Sonja Examining the Possibility of Detecting Brucella Canis from Tissue Samples Using Bruce-Ladder Multiplex PCR Assay
description	The goal of this study was to compare the results of serological and conventional bacteriological methods with the results obtained using multiplex PCR Bruce-ladder assay. Based on the obtained results, the usability of the assay was assessed in regard to rapid diagnosis of canine brucellosis directly from the samples of reproductive organs of infected dogs. Out of 225 blood samples, 33 (14.67%) had a positive agglutination reaction. In this study, out of the 225 assayed reproductive organs of dogs, B. canis was isolated from 3 samples (1.33%), while the PCR Bruce-ladder assay detected two positive samples (0.88%). Two dogs from which B. canis was isolated, an antibody titer of 1/200 was established in blood serums, and third dog from which B. canis was isolated was negative using the tube agglutination test. From a total of 225 assayed organ samples, a positive PCR reaction was obtained from two samples. The obtained results show that the tube agglutination method remains the first choice for the detection of dogs infected with B. canis. In addition, whenever possible, it is necessary to try isolation. It is desirable to attempt the detection of B. canis in tissues using PCR, but the results may not be treated as definitive and reliable.
format	article
author	Stević Nataša Mišić Dušan Bogunović Danica Matović Kazimir Valčić Miroslav Milovanović Milovan Radojičić Sonja
author_facet	Stević Nataša Mišić Dušan Bogunović Danica Matović Kazimir Valčić Miroslav Milovanović Milovan Radojičić Sonja
author_sort	Stević Nataša
title	Examining the Possibility of Detecting Brucella Canis from Tissue Samples Using Bruce-Ladder Multiplex PCR Assay
title_short	Examining the Possibility of Detecting Brucella Canis from Tissue Samples Using Bruce-Ladder Multiplex PCR Assay
title_full	Examining the Possibility of Detecting Brucella Canis from Tissue Samples Using Bruce-Ladder Multiplex PCR Assay
title_fullStr	Examining the Possibility of Detecting Brucella Canis from Tissue Samples Using Bruce-Ladder Multiplex PCR Assay
title_full_unstemmed	Examining the Possibility of Detecting Brucella Canis from Tissue Samples Using Bruce-Ladder Multiplex PCR Assay
title_sort	examining the possibility of detecting brucella canis from tissue samples using bruce-ladder multiplex pcr assay
publisher	Sciendo
publishDate	2017
url	https://doaj.org/article/d9cfd33c0aef4b6989fcbd6de8423bb7
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Examining the Possibility of Detecting Brucella Canis from Tissue Samples Using Bruce-Ladder Multiplex PCR Assay

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