Partridge and embryonated partridge egg as new preclinical models for candidiasis

Abstract Candida albicans (C. albicans) is the most common cause of candidiasis in humans and animals. This study was established to a new experimental infection model for systemic candidiasis using partridge and embryonated partridge egg. First, we tested the induction of systemic candidiasis in pa...

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Autores principales:	Hadi Tavakkoli, Ahmad Khosravi, Iraj Sharifi, Zohreh Salari, Ehsan Salarkia, Reza Kheirandish, Kazem Dehghantalebi, Maziar Jajarmi, Seyedeh Saedeh Mosallanejad, Shahriar Dabiri, Alireza Keyhani
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d9dbf951d1a8411ea3f6a7f4005b6fff
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spelling	oai:doaj.org-article:d9dbf951d1a8411ea3f6a7f4005b6fff2021-12-02T13:57:05ZPartridge and embryonated partridge egg as new preclinical models for candidiasis10.1038/s41598-021-81592-y2045-2322https://doaj.org/article/d9dbf951d1a8411ea3f6a7f4005b6fff2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81592-yhttps://doaj.org/toc/2045-2322Abstract Candida albicans (C. albicans) is the most common cause of candidiasis in humans and animals. This study was established to a new experimental infection model for systemic candidiasis using partridge and embryonated partridge egg. First, we tested the induction of systemic candidiasis in partridge and embryonated partridge egg. Finally, interaction between virulence factors of C. albicans and Bcl-2 family members was predicted. We observed that embryonic infection causes a decrease in survival time and at later embryonic days (11–12th), embryos showed lesions. Morphometric analysis of the extra-embryonic membrane (EEM) vasculature showed that vascular apoptotic effect of C. albicans was revealed by a significant reduction in capillary area. In immunohistochemistry assay, low expression of Bcl-2 and increased expression of Bax confirmed apoptosis. The gene expression of Bax and Bcl-2 was also altered in fungi-exposed EEM. Ourin silico simulation has shown an accurate interaction between aspartic proteinase, polyamine oxidase, Bcl-2 and BAX. We observed that the disease was associated with adverse consequences, which were similar to human candidiasis. Acquired results support the idea that partridge and embryonated partridge egg can be utilized as appropriate preclinical models to investigate the pathological effects of candidiasis.Hadi TavakkoliAhmad KhosraviIraj SharifiZohreh SalariEhsan SalarkiaReza KheirandishKazem DehghantalebiMaziar JajarmiSeyedeh Saedeh MosallanejadShahriar DabiriAlireza KeyhaniNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hadi Tavakkoli Ahmad Khosravi Iraj Sharifi Zohreh Salari Ehsan Salarkia Reza Kheirandish Kazem Dehghantalebi Maziar Jajarmi Seyedeh Saedeh Mosallanejad Shahriar Dabiri Alireza Keyhani Partridge and embryonated partridge egg as new preclinical models for candidiasis
description	Abstract Candida albicans (C. albicans) is the most common cause of candidiasis in humans and animals. This study was established to a new experimental infection model for systemic candidiasis using partridge and embryonated partridge egg. First, we tested the induction of systemic candidiasis in partridge and embryonated partridge egg. Finally, interaction between virulence factors of C. albicans and Bcl-2 family members was predicted. We observed that embryonic infection causes a decrease in survival time and at later embryonic days (11–12th), embryos showed lesions. Morphometric analysis of the extra-embryonic membrane (EEM) vasculature showed that vascular apoptotic effect of C. albicans was revealed by a significant reduction in capillary area. In immunohistochemistry assay, low expression of Bcl-2 and increased expression of Bax confirmed apoptosis. The gene expression of Bax and Bcl-2 was also altered in fungi-exposed EEM. Ourin silico simulation has shown an accurate interaction between aspartic proteinase, polyamine oxidase, Bcl-2 and BAX. We observed that the disease was associated with adverse consequences, which were similar to human candidiasis. Acquired results support the idea that partridge and embryonated partridge egg can be utilized as appropriate preclinical models to investigate the pathological effects of candidiasis.
format	article
author	Hadi Tavakkoli Ahmad Khosravi Iraj Sharifi Zohreh Salari Ehsan Salarkia Reza Kheirandish Kazem Dehghantalebi Maziar Jajarmi Seyedeh Saedeh Mosallanejad Shahriar Dabiri Alireza Keyhani
author_facet	Hadi Tavakkoli Ahmad Khosravi Iraj Sharifi Zohreh Salari Ehsan Salarkia Reza Kheirandish Kazem Dehghantalebi Maziar Jajarmi Seyedeh Saedeh Mosallanejad Shahriar Dabiri Alireza Keyhani
author_sort	Hadi Tavakkoli
title	Partridge and embryonated partridge egg as new preclinical models for candidiasis
title_short	Partridge and embryonated partridge egg as new preclinical models for candidiasis
title_full	Partridge and embryonated partridge egg as new preclinical models for candidiasis
title_fullStr	Partridge and embryonated partridge egg as new preclinical models for candidiasis
title_full_unstemmed	Partridge and embryonated partridge egg as new preclinical models for candidiasis
title_sort	partridge and embryonated partridge egg as new preclinical models for candidiasis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d9dbf951d1a8411ea3f6a7f4005b6fff
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Partridge and embryonated partridge egg as new preclinical models for candidiasis

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