Skin fibroblasts from patients with type 1 diabetes (T1D) can be chemically transdifferentiated into insulin-expressing clusters: a transgene-free approach.

The conversion of differentiated cells into insulin-producing cells is a promising approach for the autologous replacement of pancreatic cells in patients with type 1 diabetes (T1D). At present, cellular reprogramming strategies encompass ethical problems, epigenetic failure or teratoma formation, w...

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Autores principales:	Federico Pereyra-Bonnet, María L Gimeno, Nelson R Argumedo, Marcelo Ielpi, Johana A Cardozo, Carla A Giménez, Sung-Ho Hyon, Marta Balzaretti, Mónica Loresi, Patricia Fainstein-Day, León E Litwak, Pablo F Argibay
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d9dd9b23694d426caa0c1df6aa3f4827
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spelling	oai:doaj.org-article:d9dd9b23694d426caa0c1df6aa3f48272021-11-11T08:21:42ZSkin fibroblasts from patients with type 1 diabetes (T1D) can be chemically transdifferentiated into insulin-expressing clusters: a transgene-free approach.1932-620310.1371/journal.pone.0100369https://doaj.org/article/d9dd9b23694d426caa0c1df6aa3f48272014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24963634/?tool=EBIhttps://doaj.org/toc/1932-6203The conversion of differentiated cells into insulin-producing cells is a promising approach for the autologous replacement of pancreatic cells in patients with type 1 diabetes (T1D). At present, cellular reprogramming strategies encompass ethical problems, epigenetic failure or teratoma formation, which has prompted the development of new approaches. Here, we report a novel technique for the conversion of skin fibroblasts from T1D patients into insulin-expressing clusters using only drug-based induction. Our results demonstrate that skin fibroblasts from diabetic patients have pancreatic differentiation capacities and avoid the necessity of using transgenic strategies, stem cell sources or global demethylation steps. These findings open new possibilities for studying diabetes mechanisms, drug screenings and ultimately autologous transgenic-free regenerative medicine therapies in patients with T1D.Federico Pereyra-BonnetMaría L GimenoNelson R ArgumedoMarcelo IelpiJohana A CardozoCarla A GiménezSung-Ho HyonMarta BalzarettiMónica LoresiPatricia Fainstein-DayLeón E LitwakPablo F ArgibayPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 6, p e100369 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Federico Pereyra-Bonnet María L Gimeno Nelson R Argumedo Marcelo Ielpi Johana A Cardozo Carla A Giménez Sung-Ho Hyon Marta Balzaretti Mónica Loresi Patricia Fainstein-Day León E Litwak Pablo F Argibay Skin fibroblasts from patients with type 1 diabetes (T1D) can be chemically transdifferentiated into insulin-expressing clusters: a transgene-free approach.
description	The conversion of differentiated cells into insulin-producing cells is a promising approach for the autologous replacement of pancreatic cells in patients with type 1 diabetes (T1D). At present, cellular reprogramming strategies encompass ethical problems, epigenetic failure or teratoma formation, which has prompted the development of new approaches. Here, we report a novel technique for the conversion of skin fibroblasts from T1D patients into insulin-expressing clusters using only drug-based induction. Our results demonstrate that skin fibroblasts from diabetic patients have pancreatic differentiation capacities and avoid the necessity of using transgenic strategies, stem cell sources or global demethylation steps. These findings open new possibilities for studying diabetes mechanisms, drug screenings and ultimately autologous transgenic-free regenerative medicine therapies in patients with T1D.
format	article
author	Federico Pereyra-Bonnet María L Gimeno Nelson R Argumedo Marcelo Ielpi Johana A Cardozo Carla A Giménez Sung-Ho Hyon Marta Balzaretti Mónica Loresi Patricia Fainstein-Day León E Litwak Pablo F Argibay
author_facet	Federico Pereyra-Bonnet María L Gimeno Nelson R Argumedo Marcelo Ielpi Johana A Cardozo Carla A Giménez Sung-Ho Hyon Marta Balzaretti Mónica Loresi Patricia Fainstein-Day León E Litwak Pablo F Argibay
author_sort	Federico Pereyra-Bonnet
title	Skin fibroblasts from patients with type 1 diabetes (T1D) can be chemically transdifferentiated into insulin-expressing clusters: a transgene-free approach.
title_short	Skin fibroblasts from patients with type 1 diabetes (T1D) can be chemically transdifferentiated into insulin-expressing clusters: a transgene-free approach.
title_full	Skin fibroblasts from patients with type 1 diabetes (T1D) can be chemically transdifferentiated into insulin-expressing clusters: a transgene-free approach.
title_fullStr	Skin fibroblasts from patients with type 1 diabetes (T1D) can be chemically transdifferentiated into insulin-expressing clusters: a transgene-free approach.
title_full_unstemmed	Skin fibroblasts from patients with type 1 diabetes (T1D) can be chemically transdifferentiated into insulin-expressing clusters: a transgene-free approach.
title_sort	skin fibroblasts from patients with type 1 diabetes (t1d) can be chemically transdifferentiated into insulin-expressing clusters: a transgene-free approach.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/d9dd9b23694d426caa0c1df6aa3f4827
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Skin fibroblasts from patients with type 1 diabetes (T1D) can be chemically transdifferentiated into insulin-expressing clusters: a transgene-free approach.

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