PKM2 Is a Potential Diagnostic and Therapeutic Target for Retinitis Pigmentosa

Retinitis pigmentosa (RP) is a major cause of blindness that is difficult to diagnose and treat. PKM2, a subtype of pyruvate kinase, is strongly associated with oxidative stress and is expressed in photoreceptors. We investigated whether PKM2 reduces photoreceptor cell apoptosis and evaluated possib...

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Autores principales:	Peiwen Zhu, Qian Yang, Gang Li, Qing Chang
Formato:	article
Lenguaje:	EN
Publicado:	Hindawi Limited 2021
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/d9e1edc0584f43cea75cf6304e718862
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spelling	oai:doaj.org-article:d9e1edc0584f43cea75cf6304e7188622021-11-22T01:11:04ZPKM2 Is a Potential Diagnostic and Therapeutic Target for Retinitis Pigmentosa1875-863010.1155/2021/1602797https://doaj.org/article/d9e1edc0584f43cea75cf6304e7188622021-01-01T00:00:00Zhttp://dx.doi.org/10.1155/2021/1602797https://doaj.org/toc/1875-8630Retinitis pigmentosa (RP) is a major cause of blindness that is difficult to diagnose and treat. PKM2, a subtype of pyruvate kinase, is strongly associated with oxidative stress and is expressed in photoreceptors. We investigated whether PKM2 reduces photoreceptor cell apoptosis and evaluated possible antiapoptotic mechanisms in RP. We established RP models by exposing 661W cells to blue light and modulated PKM2 activity using a PKM2 inhibitor. We measured the apoptosis rates using calcein-acetoxymethyl ester/propidium iodide double staining and Cell Counting Kit-8, the oxidative stress levels using a reactive oxygen species assay, and the changes in protein expression by western blotting. Photodamage increased PKM2 expression, cellular oxidative stress, and apoptosis of 661W cells. PKM2 inhibition significantly reduced the levels of apoptosis and oxidative stress induced by photodamage. Our data suggest that PKM2 is a potential disease marker and therapeutic target for RP.Peiwen ZhuQian YangGang LiQing ChangHindawi LimitedarticleMedicine (General)R5-920ENDisease Markers, Vol 2021 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Peiwen Zhu Qian Yang Gang Li Qing Chang PKM2 Is a Potential Diagnostic and Therapeutic Target for Retinitis Pigmentosa
description	Retinitis pigmentosa (RP) is a major cause of blindness that is difficult to diagnose and treat. PKM2, a subtype of pyruvate kinase, is strongly associated with oxidative stress and is expressed in photoreceptors. We investigated whether PKM2 reduces photoreceptor cell apoptosis and evaluated possible antiapoptotic mechanisms in RP. We established RP models by exposing 661W cells to blue light and modulated PKM2 activity using a PKM2 inhibitor. We measured the apoptosis rates using calcein-acetoxymethyl ester/propidium iodide double staining and Cell Counting Kit-8, the oxidative stress levels using a reactive oxygen species assay, and the changes in protein expression by western blotting. Photodamage increased PKM2 expression, cellular oxidative stress, and apoptosis of 661W cells. PKM2 inhibition significantly reduced the levels of apoptosis and oxidative stress induced by photodamage. Our data suggest that PKM2 is a potential disease marker and therapeutic target for RP.
format	article
author	Peiwen Zhu Qian Yang Gang Li Qing Chang
author_facet	Peiwen Zhu Qian Yang Gang Li Qing Chang
author_sort	Peiwen Zhu
title	PKM2 Is a Potential Diagnostic and Therapeutic Target for Retinitis Pigmentosa
title_short	PKM2 Is a Potential Diagnostic and Therapeutic Target for Retinitis Pigmentosa
title_full	PKM2 Is a Potential Diagnostic and Therapeutic Target for Retinitis Pigmentosa
title_fullStr	PKM2 Is a Potential Diagnostic and Therapeutic Target for Retinitis Pigmentosa
title_full_unstemmed	PKM2 Is a Potential Diagnostic and Therapeutic Target for Retinitis Pigmentosa
title_sort	pkm2 is a potential diagnostic and therapeutic target for retinitis pigmentosa
publisher	Hindawi Limited
publishDate	2021
url	https://doaj.org/article/d9e1edc0584f43cea75cf6304e718862
work_keys_str_mv	AT peiwenzhu pkm2isapotentialdiagnosticandtherapeutictargetforretinitispigmentosa AT qianyang pkm2isapotentialdiagnosticandtherapeutictargetforretinitispigmentosa AT gangli pkm2isapotentialdiagnosticandtherapeutictargetforretinitispigmentosa AT qingchang pkm2isapotentialdiagnosticandtherapeutictargetforretinitispigmentosa
_version_	1718418311195131904

PKM2 Is a Potential Diagnostic and Therapeutic Target for Retinitis Pigmentosa

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