Engineering of an angiogenic niche by perfusion culture of adipose-derived stromal vascular fraction cells

Engineering of an angiogenic niche by perfusion culture of adipose-derived stromal vascular fraction cells

Abstract In vitro recapitulation of an organotypic stromal environment, enabling efficient angiogenesis, is crucial to investigate and possibly improve vascularization in regenerative medicine. Our study aims at engineering the complexity of a vascular milieu including multiple cell-types, a stromal...

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Autores principales: Giulia Cerino, Emanuele Gaudiello, Manuele Giuseppe Muraro, Friedrich Eckstein, Ivan Martin, Arnaud Scherberich, Anna Marsano
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2017
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Acceso en línea:https://doaj.org/article/d9ee2767555543b8b8347a5ab7b44605
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spelling oai:doaj.org-article:d9ee2767555543b8b8347a5ab7b446052021-12-02T15:06:00ZEngineering of an angiogenic niche by perfusion culture of adipose-derived stromal vascular fraction cells10.1038/s41598-017-13882-32045-2322https://doaj.org/article/d9ee2767555543b8b8347a5ab7b446052017-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-13882-3https://doaj.org/toc/2045-2322Abstract In vitro recapitulation of an organotypic stromal environment, enabling efficient angiogenesis, is crucial to investigate and possibly improve vascularization in regenerative medicine. Our study aims at engineering the complexity of a vascular milieu including multiple cell-types, a stromal extracellular matrix (ECM), and molecular signals. For this purpose, the human adipose stromal vascular fraction (SVF), composed of a heterogeneous mix of pericytes, endothelial/stromal progenitor cells, was cultured under direct perfusion flow on three-dimensional (3D) collagen scaffolds. Perfusion culture of SVF-cells reproducibly promoted in vitro the early formation of a capillary-like network, embedded within an ECM backbone, and the release of numerous pro-angiogenic factors. Compared to static cultures, perfusion-based engineered constructs were more rapidly vascularized and supported a superior survival of delivered cells upon in vivo ectopic implantation. This was likely mediated by pericytes, whose number was significantly higher (4.5-fold) under perfusion and whose targeted depletion resulted in lower efficiency of vascularization, with an increased host foreign body reaction. 3D-perfusion culture of SVF-cells leads to the engineering of a specialized milieu, here defined as an angiogenic niche. This system could serve as a model to investigate multi-cellular interactions in angiogenesis, and as a module supporting increased grafted cell survival in regenerative medicine.Giulia CerinoEmanuele GaudielloManuele Giuseppe MuraroFriedrich EcksteinIvan MartinArnaud ScherberichAnna MarsanoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Giulia Cerino
Emanuele Gaudiello
Manuele Giuseppe Muraro
Friedrich Eckstein
Ivan Martin
Arnaud Scherberich
Anna Marsano
Engineering of an angiogenic niche by perfusion culture of adipose-derived stromal vascular fraction cells
description Abstract In vitro recapitulation of an organotypic stromal environment, enabling efficient angiogenesis, is crucial to investigate and possibly improve vascularization in regenerative medicine. Our study aims at engineering the complexity of a vascular milieu including multiple cell-types, a stromal extracellular matrix (ECM), and molecular signals. For this purpose, the human adipose stromal vascular fraction (SVF), composed of a heterogeneous mix of pericytes, endothelial/stromal progenitor cells, was cultured under direct perfusion flow on three-dimensional (3D) collagen scaffolds. Perfusion culture of SVF-cells reproducibly promoted in vitro the early formation of a capillary-like network, embedded within an ECM backbone, and the release of numerous pro-angiogenic factors. Compared to static cultures, perfusion-based engineered constructs were more rapidly vascularized and supported a superior survival of delivered cells upon in vivo ectopic implantation. This was likely mediated by pericytes, whose number was significantly higher (4.5-fold) under perfusion and whose targeted depletion resulted in lower efficiency of vascularization, with an increased host foreign body reaction. 3D-perfusion culture of SVF-cells leads to the engineering of a specialized milieu, here defined as an angiogenic niche. This system could serve as a model to investigate multi-cellular interactions in angiogenesis, and as a module supporting increased grafted cell survival in regenerative medicine.
format article
author Giulia Cerino
Emanuele Gaudiello
Manuele Giuseppe Muraro
Friedrich Eckstein
Ivan Martin
Arnaud Scherberich
Anna Marsano
author_facet Giulia Cerino
Emanuele Gaudiello
Manuele Giuseppe Muraro
Friedrich Eckstein
Ivan Martin
Arnaud Scherberich
Anna Marsano
author_sort Giulia Cerino
title Engineering of an angiogenic niche by perfusion culture of adipose-derived stromal vascular fraction cells
title_short Engineering of an angiogenic niche by perfusion culture of adipose-derived stromal vascular fraction cells
title_full Engineering of an angiogenic niche by perfusion culture of adipose-derived stromal vascular fraction cells
title_fullStr Engineering of an angiogenic niche by perfusion culture of adipose-derived stromal vascular fraction cells
title_full_unstemmed Engineering of an angiogenic niche by perfusion culture of adipose-derived stromal vascular fraction cells
title_sort engineering of an angiogenic niche by perfusion culture of adipose-derived stromal vascular fraction cells
publisher Nature Portfolio
publishDate 2017
url https://doaj.org/article/d9ee2767555543b8b8347a5ab7b44605
work_keys_str_mv AT giuliacerino engineeringofanangiogenicnichebyperfusioncultureofadiposederivedstromalvascularfractioncells
AT emanuelegaudiello engineeringofanangiogenicnichebyperfusioncultureofadiposederivedstromalvascularfractioncells
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AT friedricheckstein engineeringofanangiogenicnichebyperfusioncultureofadiposederivedstromalvascularfractioncells
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AT arnaudscherberich engineeringofanangiogenicnichebyperfusioncultureofadiposederivedstromalvascularfractioncells
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