Impact of graft-versus-host disease prophylaxis on immune reconstitution in patients after allogeneic hematopoietic stem cell transplantation

The graft-versus-host disease (GVHD) is among the most common complications after hematopoietic stem cell transplantation (allo-HSCT). The main tools for GVHD prevention remain calcineurin inhibitors (cyclosporin A, tacrolimus), methotrexate, mycophenolate mofetil. Upon implementation of reduced-int...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	E. D. Mikhaltsova, N. N. Popova, M. Yu. Drokov, N. M. Kapranov, Yu. O. Davydova, V. A. Vasilieva, D. S. Dubnyak, U. V. Maslikova, I. V. Galtseva, L. A. Kuzmina, E. N. Parovichnikova, V. G. Savchenko
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2021
Materias:	bone marrow transplantation graft versus host disease post-transplant cyclophosphamide flow cytometry haploidentical transplantation immunophenotyping Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/d9f89a8ee79e4ad7b3bf668ee460f2a1
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d9f89a8ee79e4ad7b3bf668ee460f2a1
record_format	dspace
spelling	oai:doaj.org-article:d9f89a8ee79e4ad7b3bf668ee460f2a12021-11-18T08:03:51ZImpact of graft-versus-host disease prophylaxis on immune reconstitution in patients after allogeneic hematopoietic stem cell transplantation1563-06252313-741X10.15789/1563-0625-IOG-2167https://doaj.org/article/d9f89a8ee79e4ad7b3bf668ee460f2a12021-11-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/2167https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XThe graft-versus-host disease (GVHD) is among the most common complications after hematopoietic stem cell transplantation (allo-HSCT). The main tools for GVHD prevention remain calcineurin inhibitors (cyclosporin A, tacrolimus), methotrexate, mycophenolate mofetil. Upon implementation of reduced-intensity conditioning regimens, antithymocyte globulin was widely introduced. However, negative effects upon reconstitution of T-cell immunity have been noted, thus increasing risk of severe infectious complications and disease relapse. With extended practice of HSCT from alternative (partially matched or haploidentical) donors, cyclophosphamide was increasingly used. Our aim was to study reconstitution of immune cell subpopulations in the patients undergoing bone marrow transplantation (BMT), when using different GVHD prophylaxis regimens, including the schedules with post-transplant CP usage. The study concerned 44 cases classified into 2 groups. The first one included patients with standard immunosuppressive therapy, antithymocyte therapy, cyclosporine A, methotrexate, mycophenolate mofetil. The second group included the patients who received CP as immunosuppressive drug combined with other treatments (cyclosporine A, methotrexate, mycophenolate mofetil). At specified control terms, (D+14, +30, +60, +90) the blood leukocyte subpopulations were assayed by means of multicolor flow cytometry. Absolute counts of CD4+ cells in HSCT recipients treated with CP post-BMT proved to be sufficiently lower at D+14 and +30, than in those treated with classical immunosuppressive therapy. However, at later terms, (D+60, +90), these differences were not observed. Moreover, in CP-treated bone marrow recipients, absolute numbers of CD8+ cells was significantly higher, compared to the patients who received conventional GVHD prophylaxis. Reconstitution of the studied lymphocyte populations in hematopoietic cell recipients did not depend on the GVHD prophylaxis regimen. Usage of CP combined with bone marrow as a source of stem cells, brings about sufficient decrease of some cell populations (CD4+; CD8+; NK cells) at early terms post-transplant. Administration of CP combined with hematopoietic stem cells as the source of hematopoietic graft seems to be more reasonable.E. D. MikhaltsovaN. N. PopovaM. Yu. DrokovN. M. KapranovYu. O. DavydovaV. A. VasilievaD. S. DubnyakU. V. MaslikovaI. V. GaltsevaL. A. KuzminaE. N. ParovichnikovaV. G. SavchenkoSPb RAACIarticlebone marrow transplantationgraft versus host diseasepost-transplant cyclophosphamideflow cytometryhaploidentical transplantationimmunophenotypingImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 23, Iss 5, Pp 1125-1136 (2021)
institution	DOAJ
collection	DOAJ
language	RU
topic	bone marrow transplantation graft versus host disease post-transplant cyclophosphamide flow cytometry haploidentical transplantation immunophenotyping Immunologic diseases. Allergy RC581-607
spellingShingle	bone marrow transplantation graft versus host disease post-transplant cyclophosphamide flow cytometry haploidentical transplantation immunophenotyping Immunologic diseases. Allergy RC581-607 E. D. Mikhaltsova N. N. Popova M. Yu. Drokov N. M. Kapranov Yu. O. Davydova V. A. Vasilieva D. S. Dubnyak U. V. Maslikova I. V. Galtseva L. A. Kuzmina E. N. Parovichnikova V. G. Savchenko Impact of graft-versus-host disease prophylaxis on immune reconstitution in patients after allogeneic hematopoietic stem cell transplantation
description	The graft-versus-host disease (GVHD) is among the most common complications after hematopoietic stem cell transplantation (allo-HSCT). The main tools for GVHD prevention remain calcineurin inhibitors (cyclosporin A, tacrolimus), methotrexate, mycophenolate mofetil. Upon implementation of reduced-intensity conditioning regimens, antithymocyte globulin was widely introduced. However, negative effects upon reconstitution of T-cell immunity have been noted, thus increasing risk of severe infectious complications and disease relapse. With extended practice of HSCT from alternative (partially matched or haploidentical) donors, cyclophosphamide was increasingly used. Our aim was to study reconstitution of immune cell subpopulations in the patients undergoing bone marrow transplantation (BMT), when using different GVHD prophylaxis regimens, including the schedules with post-transplant CP usage. The study concerned 44 cases classified into 2 groups. The first one included patients with standard immunosuppressive therapy, antithymocyte therapy, cyclosporine A, methotrexate, mycophenolate mofetil. The second group included the patients who received CP as immunosuppressive drug combined with other treatments (cyclosporine A, methotrexate, mycophenolate mofetil). At specified control terms, (D+14, +30, +60, +90) the blood leukocyte subpopulations were assayed by means of multicolor flow cytometry. Absolute counts of CD4+ cells in HSCT recipients treated with CP post-BMT proved to be sufficiently lower at D+14 and +30, than in those treated with classical immunosuppressive therapy. However, at later terms, (D+60, +90), these differences were not observed. Moreover, in CP-treated bone marrow recipients, absolute numbers of CD8+ cells was significantly higher, compared to the patients who received conventional GVHD prophylaxis. Reconstitution of the studied lymphocyte populations in hematopoietic cell recipients did not depend on the GVHD prophylaxis regimen. Usage of CP combined with bone marrow as a source of stem cells, brings about sufficient decrease of some cell populations (CD4+; CD8+; NK cells) at early terms post-transplant. Administration of CP combined with hematopoietic stem cells as the source of hematopoietic graft seems to be more reasonable.
format	article
author	E. D. Mikhaltsova N. N. Popova M. Yu. Drokov N. M. Kapranov Yu. O. Davydova V. A. Vasilieva D. S. Dubnyak U. V. Maslikova I. V. Galtseva L. A. Kuzmina E. N. Parovichnikova V. G. Savchenko
author_facet	E. D. Mikhaltsova N. N. Popova M. Yu. Drokov N. M. Kapranov Yu. O. Davydova V. A. Vasilieva D. S. Dubnyak U. V. Maslikova I. V. Galtseva L. A. Kuzmina E. N. Parovichnikova V. G. Savchenko
author_sort	E. D. Mikhaltsova
title	Impact of graft-versus-host disease prophylaxis on immune reconstitution in patients after allogeneic hematopoietic stem cell transplantation
title_short	Impact of graft-versus-host disease prophylaxis on immune reconstitution in patients after allogeneic hematopoietic stem cell transplantation
title_full	Impact of graft-versus-host disease prophylaxis on immune reconstitution in patients after allogeneic hematopoietic stem cell transplantation
title_fullStr	Impact of graft-versus-host disease prophylaxis on immune reconstitution in patients after allogeneic hematopoietic stem cell transplantation
title_full_unstemmed	Impact of graft-versus-host disease prophylaxis on immune reconstitution in patients after allogeneic hematopoietic stem cell transplantation
title_sort	impact of graft-versus-host disease prophylaxis on immune reconstitution in patients after allogeneic hematopoietic stem cell transplantation
publisher	SPb RAACI
publishDate	2021
url	https://doaj.org/article/d9f89a8ee79e4ad7b3bf668ee460f2a1
work_keys_str_mv	AT edmikhaltsova impactofgraftversushostdiseaseprophylaxisonimmunereconstitutioninpatientsafterallogeneichematopoieticstemcelltransplantation AT nnpopova impactofgraftversushostdiseaseprophylaxisonimmunereconstitutioninpatientsafterallogeneichematopoieticstemcelltransplantation AT myudrokov impactofgraftversushostdiseaseprophylaxisonimmunereconstitutioninpatientsafterallogeneichematopoieticstemcelltransplantation AT nmkapranov impactofgraftversushostdiseaseprophylaxisonimmunereconstitutioninpatientsafterallogeneichematopoieticstemcelltransplantation AT yuodavydova impactofgraftversushostdiseaseprophylaxisonimmunereconstitutioninpatientsafterallogeneichematopoieticstemcelltransplantation AT vavasilieva impactofgraftversushostdiseaseprophylaxisonimmunereconstitutioninpatientsafterallogeneichematopoieticstemcelltransplantation AT dsdubnyak impactofgraftversushostdiseaseprophylaxisonimmunereconstitutioninpatientsafterallogeneichematopoieticstemcelltransplantation AT uvmaslikova impactofgraftversushostdiseaseprophylaxisonimmunereconstitutioninpatientsafterallogeneichematopoieticstemcelltransplantation AT ivgaltseva impactofgraftversushostdiseaseprophylaxisonimmunereconstitutioninpatientsafterallogeneichematopoieticstemcelltransplantation AT lakuzmina impactofgraftversushostdiseaseprophylaxisonimmunereconstitutioninpatientsafterallogeneichematopoieticstemcelltransplantation AT enparovichnikova impactofgraftversushostdiseaseprophylaxisonimmunereconstitutioninpatientsafterallogeneichematopoieticstemcelltransplantation AT vgsavchenko impactofgraftversushostdiseaseprophylaxisonimmunereconstitutioninpatientsafterallogeneichematopoieticstemcelltransplantation
_version_	1718422320090972160

Impact of graft-versus-host disease prophylaxis on immune reconstitution in patients after allogeneic hematopoietic stem cell transplantation

Ejemplares similares