Large-scale comparative analysis of cytogenetic markers across Lepidoptera

Abstract Fluorescence in situ hybridization (FISH) allows identification of particular chromosomes and their rearrangements. Using FISH with signal enhancement via antibody amplification and enzymatically catalysed reporter deposition, we evaluated applicability of universal cytogenetic markers, nam...

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Autores principales:	Irena Provazníková, Martina Hejníčková, Sander Visser, Martina Dalíková, Leonela Z. Carabajal Paladino, Magda Zrzavá, Anna Voleníková, František Marec, Petr Nguyen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d9fc6e1164074ab38557b6519ce0705f
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spelling	oai:doaj.org-article:d9fc6e1164074ab38557b6519ce0705f2021-12-02T17:38:27ZLarge-scale comparative analysis of cytogenetic markers across Lepidoptera10.1038/s41598-021-91665-72045-2322https://doaj.org/article/d9fc6e1164074ab38557b6519ce0705f2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91665-7https://doaj.org/toc/2045-2322Abstract Fluorescence in situ hybridization (FISH) allows identification of particular chromosomes and their rearrangements. Using FISH with signal enhancement via antibody amplification and enzymatically catalysed reporter deposition, we evaluated applicability of universal cytogenetic markers, namely 18S and 5S rDNA genes, U1 and U2 snRNA genes, and histone H3 genes, in the study of the karyotype evolution in moths and butterflies. Major rDNA underwent rather erratic evolution, which does not always reflect chromosomal changes. In contrast, the hybridization pattern of histone H3 genes was well conserved, reflecting the stable organisation of lepidopteran genomes. Unlike 5S rDNA and U1 and U2 snRNA genes which we failed to detect, except for 5S rDNA in a few representatives of early diverging lepidopteran lineages. To explain the negative FISH results, we used quantitative PCR and Southern hybridization to estimate the copy number and organization of the studied genes in selected species. The results suggested that their detection was hampered by long spacers between the genes and/or their scattered distribution. Our results question homology of 5S rDNA and U1 and U2 snRNA loci in comparative studies. We recommend the use of histone H3 in studies of karyotype evolution.Irena ProvazníkováMartina HejníčkováSander VisserMartina DalíkováLeonela Z. Carabajal PaladinoMagda ZrzaváAnna VoleníkováFrantišek MarecPetr NguyenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Irena Provazníková Martina Hejníčková Sander Visser Martina Dalíková Leonela Z. Carabajal Paladino Magda Zrzavá Anna Voleníková František Marec Petr Nguyen Large-scale comparative analysis of cytogenetic markers across Lepidoptera
description	Abstract Fluorescence in situ hybridization (FISH) allows identification of particular chromosomes and their rearrangements. Using FISH with signal enhancement via antibody amplification and enzymatically catalysed reporter deposition, we evaluated applicability of universal cytogenetic markers, namely 18S and 5S rDNA genes, U1 and U2 snRNA genes, and histone H3 genes, in the study of the karyotype evolution in moths and butterflies. Major rDNA underwent rather erratic evolution, which does not always reflect chromosomal changes. In contrast, the hybridization pattern of histone H3 genes was well conserved, reflecting the stable organisation of lepidopteran genomes. Unlike 5S rDNA and U1 and U2 snRNA genes which we failed to detect, except for 5S rDNA in a few representatives of early diverging lepidopteran lineages. To explain the negative FISH results, we used quantitative PCR and Southern hybridization to estimate the copy number and organization of the studied genes in selected species. The results suggested that their detection was hampered by long spacers between the genes and/or their scattered distribution. Our results question homology of 5S rDNA and U1 and U2 snRNA loci in comparative studies. We recommend the use of histone H3 in studies of karyotype evolution.
format	article
author	Irena Provazníková Martina Hejníčková Sander Visser Martina Dalíková Leonela Z. Carabajal Paladino Magda Zrzavá Anna Voleníková František Marec Petr Nguyen
author_facet	Irena Provazníková Martina Hejníčková Sander Visser Martina Dalíková Leonela Z. Carabajal Paladino Magda Zrzavá Anna Voleníková František Marec Petr Nguyen
author_sort	Irena Provazníková
title	Large-scale comparative analysis of cytogenetic markers across Lepidoptera
title_short	Large-scale comparative analysis of cytogenetic markers across Lepidoptera
title_full	Large-scale comparative analysis of cytogenetic markers across Lepidoptera
title_fullStr	Large-scale comparative analysis of cytogenetic markers across Lepidoptera
title_full_unstemmed	Large-scale comparative analysis of cytogenetic markers across Lepidoptera
title_sort	large-scale comparative analysis of cytogenetic markers across lepidoptera
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d9fc6e1164074ab38557b6519ce0705f
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Large-scale comparative analysis of cytogenetic markers across Lepidoptera

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