Evaluation of Metarhizium rileyi Farlow (Samson) impregnated with azadirachtin and indoxacarb against Helicoverpa armigera (Hubner)

Abstract Background Entomopathogenic fungi are the most versatile having a wide host range, capable of infecting insects at different developmental stages. In the present study, Metarhizium rileyi, at the concentrations of 102, 103, 104, 105, 106, 107 and 108 conidia/ml and sub-lethal concentrations...

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Autores principales:	Bhisham Dev, Subhash Chander Verma, Prem Lal Sharma, Rajeshwar Singh Chandel, Mahesh Balaso Gaikwad, Tanuja Banshtu, Priyanka Sharma
Formato:	article
Lenguaje:	EN
Publicado:	SpringerOpen 2021
Materias:	Helicoverpa armigera Entomopathogenic fungus Metarhizium rileyi Azadirachtin Indoxacarb Evaluation Agriculture S
Acceso en línea:	https://doaj.org/article/d9fde211375147b08988ee0d8e08287b
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spelling	oai:doaj.org-article:d9fde211375147b08988ee0d8e08287b2021-11-14T12:07:58ZEvaluation of Metarhizium rileyi Farlow (Samson) impregnated with azadirachtin and indoxacarb against Helicoverpa armigera (Hubner)10.1186/s41938-021-00487-22536-9342https://doaj.org/article/d9fde211375147b08988ee0d8e08287b2021-11-01T00:00:00Zhttps://doi.org/10.1186/s41938-021-00487-2https://doaj.org/toc/2536-9342Abstract Background Entomopathogenic fungi are the most versatile having a wide host range, capable of infecting insects at different developmental stages. In the present study, Metarhizium rileyi, at the concentrations of 102, 103, 104, 105, 106, 107 and 108 conidia/ml and sub-lethal concentrations of azadirachtin (1.02 and 1.53 ppm) and indoxacarb (0.72 ppm) were evaluated against the 1st, 2nd, 3rd, 4th and 5th larval instars of Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae) under laboratory conditions. Results M. rileyi applied at 106 conidia/ml caused a maximum mortality of 83.33 and 80.00% of 1st and 2nd larval instars of H. armigera, respectively. The maximum mortality of 3rd, 4th and 5th larval instars of H. armigera with 108 conidia/ml of M. rileyi was 83.33, 76.67 and 53.33%, respectively. When M. rileyi blended with azadirachtin at 1.02 ppm, the highest mortality rate of 86.21% at 106 conidia/ml against 2nd instar larvae was resulted. Similarly, M. rileyi applied at 108 conidia /ml mixed with azadirachtin (1.53 ppm) showed 89.66% mortality of 3rd instar larvae. The 2nd instar larvae treated with M. rileyi at 106 conidia/ml, mixed with indoxacarb (0.72 ppm), the corrected mortality rate was 82.14%. Concentration mortality response of 3rd instar larvae to M. rileyi blended with indoxacarb (0.72 ppm) was 85.71% at 108 conidia/ml. The median lethal concentration (LC50) values were 5.51 × 103, 1.86 × 104, 2.81 × 105 and 5.55 × 105 conidia/ml for 1st, 2nd, 3rd and 4th larval instars, respectively, after 7 days of treatment. M. rileyi when mixed with sub-lethal concentrations of azadirachtin (1.02 ppm) and indoxacarb (0.72 ppm) resulted LC50 values of 1.09 × 104 conidia/ml and 1.37 × 104 conidia/ml against 2nd instar larvae, respectively, after 24 hours. Similarly, M. rileyi mixed with sub-lethal concentrations of azadirachtin (1.53 ppm) and indoxacarb (0.72 ppm) resulted LC50 values of 3.12 × 108 and 3.06 × 105 conidia/ml against 3rd instar larvae, respectively, after 24 hours. The study revealed that the susceptibility of larvae decreased in case of large larval instars. Conclusions M. rileyi can be utilized as one of the component of Integrated Pest Management Program for the eco-friendly management of H. armigera. As the application of M. rileyi @ 107 conidia/ml alone or in combination with azadirachtin (1.02 and 1.53 ppm) or indoxacarb (0.72 ppm) resulted to the highest mortality.Bhisham DevSubhash Chander VermaPrem Lal SharmaRajeshwar Singh ChandelMahesh Balaso GaikwadTanuja BanshtuPriyanka SharmaSpringerOpenarticleHelicoverpa armigeraEntomopathogenic fungusMetarhizium rileyiAzadirachtinIndoxacarbEvaluationAgricultureSENEgyptian Journal of Biological Pest Control, Vol 31, Iss 1, Pp 1-7 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Helicoverpa armigera Entomopathogenic fungus Metarhizium rileyi Azadirachtin Indoxacarb Evaluation Agriculture S
spellingShingle	Helicoverpa armigera Entomopathogenic fungus Metarhizium rileyi Azadirachtin Indoxacarb Evaluation Agriculture S Bhisham Dev Subhash Chander Verma Prem Lal Sharma Rajeshwar Singh Chandel Mahesh Balaso Gaikwad Tanuja Banshtu Priyanka Sharma Evaluation of Metarhizium rileyi Farlow (Samson) impregnated with azadirachtin and indoxacarb against Helicoverpa armigera (Hubner)
description	Abstract Background Entomopathogenic fungi are the most versatile having a wide host range, capable of infecting insects at different developmental stages. In the present study, Metarhizium rileyi, at the concentrations of 102, 103, 104, 105, 106, 107 and 108 conidia/ml and sub-lethal concentrations of azadirachtin (1.02 and 1.53 ppm) and indoxacarb (0.72 ppm) were evaluated against the 1st, 2nd, 3rd, 4th and 5th larval instars of Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae) under laboratory conditions. Results M. rileyi applied at 106 conidia/ml caused a maximum mortality of 83.33 and 80.00% of 1st and 2nd larval instars of H. armigera, respectively. The maximum mortality of 3rd, 4th and 5th larval instars of H. armigera with 108 conidia/ml of M. rileyi was 83.33, 76.67 and 53.33%, respectively. When M. rileyi blended with azadirachtin at 1.02 ppm, the highest mortality rate of 86.21% at 106 conidia/ml against 2nd instar larvae was resulted. Similarly, M. rileyi applied at 108 conidia /ml mixed with azadirachtin (1.53 ppm) showed 89.66% mortality of 3rd instar larvae. The 2nd instar larvae treated with M. rileyi at 106 conidia/ml, mixed with indoxacarb (0.72 ppm), the corrected mortality rate was 82.14%. Concentration mortality response of 3rd instar larvae to M. rileyi blended with indoxacarb (0.72 ppm) was 85.71% at 108 conidia/ml. The median lethal concentration (LC50) values were 5.51 × 103, 1.86 × 104, 2.81 × 105 and 5.55 × 105 conidia/ml for 1st, 2nd, 3rd and 4th larval instars, respectively, after 7 days of treatment. M. rileyi when mixed with sub-lethal concentrations of azadirachtin (1.02 ppm) and indoxacarb (0.72 ppm) resulted LC50 values of 1.09 × 104 conidia/ml and 1.37 × 104 conidia/ml against 2nd instar larvae, respectively, after 24 hours. Similarly, M. rileyi mixed with sub-lethal concentrations of azadirachtin (1.53 ppm) and indoxacarb (0.72 ppm) resulted LC50 values of 3.12 × 108 and 3.06 × 105 conidia/ml against 3rd instar larvae, respectively, after 24 hours. The study revealed that the susceptibility of larvae decreased in case of large larval instars. Conclusions M. rileyi can be utilized as one of the component of Integrated Pest Management Program for the eco-friendly management of H. armigera. As the application of M. rileyi @ 107 conidia/ml alone or in combination with azadirachtin (1.02 and 1.53 ppm) or indoxacarb (0.72 ppm) resulted to the highest mortality.
format	article
author	Bhisham Dev Subhash Chander Verma Prem Lal Sharma Rajeshwar Singh Chandel Mahesh Balaso Gaikwad Tanuja Banshtu Priyanka Sharma
author_facet	Bhisham Dev Subhash Chander Verma Prem Lal Sharma Rajeshwar Singh Chandel Mahesh Balaso Gaikwad Tanuja Banshtu Priyanka Sharma
author_sort	Bhisham Dev
title	Evaluation of Metarhizium rileyi Farlow (Samson) impregnated with azadirachtin and indoxacarb against Helicoverpa armigera (Hubner)
title_short	Evaluation of Metarhizium rileyi Farlow (Samson) impregnated with azadirachtin and indoxacarb against Helicoverpa armigera (Hubner)
title_full	Evaluation of Metarhizium rileyi Farlow (Samson) impregnated with azadirachtin and indoxacarb against Helicoverpa armigera (Hubner)
title_fullStr	Evaluation of Metarhizium rileyi Farlow (Samson) impregnated with azadirachtin and indoxacarb against Helicoverpa armigera (Hubner)
title_full_unstemmed	Evaluation of Metarhizium rileyi Farlow (Samson) impregnated with azadirachtin and indoxacarb against Helicoverpa armigera (Hubner)
title_sort	evaluation of metarhizium rileyi farlow (samson) impregnated with azadirachtin and indoxacarb against helicoverpa armigera (hubner)
publisher	SpringerOpen
publishDate	2021
url	https://doaj.org/article/d9fde211375147b08988ee0d8e08287b
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Evaluation of Metarhizium rileyi Farlow (Samson) impregnated with azadirachtin and indoxacarb against Helicoverpa armigera (Hubner)

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