Expression levels of LCORL are associated with body size in horses.

Body size is an important characteristic for horses of various breeds and essential for the classification of ponies concerning the limit value of 148 cm (58.27 inches) height at the withers. Genome-wide association analyses revealed the highest associated quantitative trait locus for height at the...

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Autores principales:	Julia Metzger, Rahel Schrimpf, Ute Philipp, Ottmar Distl
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/da0001b99ffd4b02a2f60669b0233328
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spelling	oai:doaj.org-article:da0001b99ffd4b02a2f60669b02333282021-11-18T07:57:37ZExpression levels of LCORL are associated with body size in horses.1932-620310.1371/journal.pone.0056497https://doaj.org/article/da0001b99ffd4b02a2f60669b02333282013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23418579/?tool=EBIhttps://doaj.org/toc/1932-6203Body size is an important characteristic for horses of various breeds and essential for the classification of ponies concerning the limit value of 148 cm (58.27 inches) height at the withers. Genome-wide association analyses revealed the highest associated quantitative trait locus for height at the withers on horse chromosome (ECA) 3 upstream of the candidate gene LCORL. Using 214 Hanoverian horses genotyped on the Illumina equine SNP50 BeadChip and 42 different horse breeds across all size ranges, we confirmed the highly associated single nucleotide polymorphism BIEC2-808543 (-log(10)P = 8.3) and the adjacent gene LCORL as the most promising candidate for body size. We investigated the relative expression levels of LCORL and its two neighbouring genes NCAPG and DCAF16 using quantitative real-time PCR (RT-qPCR). We could demonstrate a significant association of the relative LCORL expression levels with the size of the horses and the BIEC2-808543 genotypes within and across horse breeds. In heterozygous C/T-horses expression levels of LCORL were significantly decreased by 40% and in homozygous C/C-horses by 56% relative to the smaller T/T-horses. Bioinformatic analyses indicated that this SNP T>C mutation is disrupting a putative binding site of the transcription factor TFIID which is important for the transcription process of genes involved in skeletal bone development. Thus, our findings suggest that expression levels of LCORL play a key role for body size within and across horse breeds and regulation of the expression of LCORL is associated with genetic variants of BIEC2-808543. This is the first functional study for a body size regulating polymorphism in horses and a further step to unravel the mechanisms for understanding the genetic regulation of body size in horses.Julia MetzgerRahel SchrimpfUte PhilippOttmar DistlPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 2, p e56497 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Julia Metzger Rahel Schrimpf Ute Philipp Ottmar Distl Expression levels of LCORL are associated with body size in horses.
description	Body size is an important characteristic for horses of various breeds and essential for the classification of ponies concerning the limit value of 148 cm (58.27 inches) height at the withers. Genome-wide association analyses revealed the highest associated quantitative trait locus for height at the withers on horse chromosome (ECA) 3 upstream of the candidate gene LCORL. Using 214 Hanoverian horses genotyped on the Illumina equine SNP50 BeadChip and 42 different horse breeds across all size ranges, we confirmed the highly associated single nucleotide polymorphism BIEC2-808543 (-log(10)P = 8.3) and the adjacent gene LCORL as the most promising candidate for body size. We investigated the relative expression levels of LCORL and its two neighbouring genes NCAPG and DCAF16 using quantitative real-time PCR (RT-qPCR). We could demonstrate a significant association of the relative LCORL expression levels with the size of the horses and the BIEC2-808543 genotypes within and across horse breeds. In heterozygous C/T-horses expression levels of LCORL were significantly decreased by 40% and in homozygous C/C-horses by 56% relative to the smaller T/T-horses. Bioinformatic analyses indicated that this SNP T>C mutation is disrupting a putative binding site of the transcription factor TFIID which is important for the transcription process of genes involved in skeletal bone development. Thus, our findings suggest that expression levels of LCORL play a key role for body size within and across horse breeds and regulation of the expression of LCORL is associated with genetic variants of BIEC2-808543. This is the first functional study for a body size regulating polymorphism in horses and a further step to unravel the mechanisms for understanding the genetic regulation of body size in horses.
format	article
author	Julia Metzger Rahel Schrimpf Ute Philipp Ottmar Distl
author_facet	Julia Metzger Rahel Schrimpf Ute Philipp Ottmar Distl
author_sort	Julia Metzger
title	Expression levels of LCORL are associated with body size in horses.
title_short	Expression levels of LCORL are associated with body size in horses.
title_full	Expression levels of LCORL are associated with body size in horses.
title_fullStr	Expression levels of LCORL are associated with body size in horses.
title_full_unstemmed	Expression levels of LCORL are associated with body size in horses.
title_sort	expression levels of lcorl are associated with body size in horses.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/da0001b99ffd4b02a2f60669b0233328
work_keys_str_mv	AT juliametzger expressionlevelsoflcorlareassociatedwithbodysizeinhorses AT rahelschrimpf expressionlevelsoflcorlareassociatedwithbodysizeinhorses AT utephilipp expressionlevelsoflcorlareassociatedwithbodysizeinhorses AT ottmardistl expressionlevelsoflcorlareassociatedwithbodysizeinhorses
_version_	1718422761249964032

Expression levels of LCORL are associated with body size in horses.

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