Systematic analysis of protein turnover in primary cells

The proteome-wide characterization of proteostasis depends on robust approaches to determine protein half-lives. Here, the authors improve the accuracy and precision of mass spectrometry-based quantification, enabling reliable protein half-life determination in several non-dividing cell types.

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Autores principales:	Toby Mathieson, Holger Franken, Jan Kosinski, Nils Kurzawa, Nico Zinn, Gavain Sweetman, Daniel Poeckel, Vikram S. Ratnu, Maike Schramm, Isabelle Becher, Michael Steidel, Kyung-Min Noh, Giovanna Bergamini, Martin Beck, Marcus Bantscheff, Mikhail M. Savitski
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/da0726d0c6b44f4fa437f686bacfcd20
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spelling	oai:doaj.org-article:da0726d0c6b44f4fa437f686bacfcd202021-12-02T14:38:49ZSystematic analysis of protein turnover in primary cells10.1038/s41467-018-03106-12041-1723https://doaj.org/article/da0726d0c6b44f4fa437f686bacfcd202018-02-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-03106-1https://doaj.org/toc/2041-1723The proteome-wide characterization of proteostasis depends on robust approaches to determine protein half-lives. Here, the authors improve the accuracy and precision of mass spectrometry-based quantification, enabling reliable protein half-life determination in several non-dividing cell types.Toby MathiesonHolger FrankenJan KosinskiNils KurzawaNico ZinnGavain SweetmanDaniel PoeckelVikram S. RatnuMaike SchrammIsabelle BecherMichael SteidelKyung-Min NohGiovanna BergaminiMartin BeckMarcus BantscheffMikhail M. SavitskiNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Toby Mathieson Holger Franken Jan Kosinski Nils Kurzawa Nico Zinn Gavain Sweetman Daniel Poeckel Vikram S. Ratnu Maike Schramm Isabelle Becher Michael Steidel Kyung-Min Noh Giovanna Bergamini Martin Beck Marcus Bantscheff Mikhail M. Savitski Systematic analysis of protein turnover in primary cells
description	The proteome-wide characterization of proteostasis depends on robust approaches to determine protein half-lives. Here, the authors improve the accuracy and precision of mass spectrometry-based quantification, enabling reliable protein half-life determination in several non-dividing cell types.
format	article
author	Toby Mathieson Holger Franken Jan Kosinski Nils Kurzawa Nico Zinn Gavain Sweetman Daniel Poeckel Vikram S. Ratnu Maike Schramm Isabelle Becher Michael Steidel Kyung-Min Noh Giovanna Bergamini Martin Beck Marcus Bantscheff Mikhail M. Savitski
author_facet	Toby Mathieson Holger Franken Jan Kosinski Nils Kurzawa Nico Zinn Gavain Sweetman Daniel Poeckel Vikram S. Ratnu Maike Schramm Isabelle Becher Michael Steidel Kyung-Min Noh Giovanna Bergamini Martin Beck Marcus Bantscheff Mikhail M. Savitski
author_sort	Toby Mathieson
title	Systematic analysis of protein turnover in primary cells
title_short	Systematic analysis of protein turnover in primary cells
title_full	Systematic analysis of protein turnover in primary cells
title_fullStr	Systematic analysis of protein turnover in primary cells
title_full_unstemmed	Systematic analysis of protein turnover in primary cells
title_sort	systematic analysis of protein turnover in primary cells
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/da0726d0c6b44f4fa437f686bacfcd20
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Systematic analysis of protein turnover in primary cells

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