Proteomics analysis reveals the effect of 1α,25(OH)2VD3-glycosides on development of early testes in piglets

Abstract 1α,25(OH)2VD3 is the most active form of VD3 in animals. It plays an important role in regulating mineral metabolism but also in reproduction. Testes are the main reproductive organs of male mammals. Our research aims to reveal the effect of 1α,25(OH)2VD3-glycosides on development of early...

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Autores principales:	Haodong Chen, Kathrin Bühler, Yan Zhu, Xiongwei Nie, Wanghong Liu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/da0bd854e2ae43bb9e0f7026ba9f915e
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spelling	oai:doaj.org-article:da0bd854e2ae43bb9e0f7026ba9f915e2021-12-02T17:51:05ZProteomics analysis reveals the effect of 1α,25(OH)2VD3-glycosides on development of early testes in piglets10.1038/s41598-021-90676-82045-2322https://doaj.org/article/da0bd854e2ae43bb9e0f7026ba9f915e2021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90676-8https://doaj.org/toc/2045-2322Abstract 1α,25(OH)2VD3 is the most active form of VD3 in animals. It plays an important role in regulating mineral metabolism but also in reproduction. Testes are the main reproductive organs of male mammals. Our research aims to reveal the effect of 1α,25(OH)2VD3-glycosides on development of early testes in piglets. 140 weaned 21-day old piglets were selected. The piglets were randomly divided into four groups and were fed a commercial diet supplemented with 0, 1, 2 and 4 μg/kg of 1α,25(OH)2VD3, provided as 1α,25(OH)2VD3-glycosides. Sixty days after the start of the experiment, at piglet age 82 days, testes were harvested. The morphology and histology of early testicular development were assessed. In addition, the proteomic TMT/iTRAQ labelling technique was used to analyse the protein profile of the testes in each group. Western blotting was applied to verify the target of differentially abundant proteins (DAPs). The analysis of morphology and histology of testes showed that a certain concentration of 1α,25(OH)2VD3-glycosides had a positive and significant effect on testicular development. And the results of proteomics analysis showed that of the identified 132,715 peptides, 122,755 were unique peptides. 7852 proteins, of which 6573 proteins contain quantitative information. Screening for DAPs focused on proteins closely related to the regulation of testicular development such as steroid hormone synthesis, steroid biosynthesis, peroxisome and fatty acid metabolism pathways. These results indicated that 1α,25(OH)2VD3 is involved in the regulation of early testicular development in piglets. At the same time, these findings provide valuable information for the proteins involved in the regulation of testicular development, and help to better understand the mechanisms of 1α,25(OH)2VD3 in regulating the development of piglets’ testes.Haodong ChenKathrin BühlerYan ZhuXiongwei NieWanghong LiuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-17 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Haodong Chen Kathrin Bühler Yan Zhu Xiongwei Nie Wanghong Liu Proteomics analysis reveals the effect of 1α,25(OH)2VD3-glycosides on development of early testes in piglets
description	Abstract 1α,25(OH)2VD3 is the most active form of VD3 in animals. It plays an important role in regulating mineral metabolism but also in reproduction. Testes are the main reproductive organs of male mammals. Our research aims to reveal the effect of 1α,25(OH)2VD3-glycosides on development of early testes in piglets. 140 weaned 21-day old piglets were selected. The piglets were randomly divided into four groups and were fed a commercial diet supplemented with 0, 1, 2 and 4 μg/kg of 1α,25(OH)2VD3, provided as 1α,25(OH)2VD3-glycosides. Sixty days after the start of the experiment, at piglet age 82 days, testes were harvested. The morphology and histology of early testicular development were assessed. In addition, the proteomic TMT/iTRAQ labelling technique was used to analyse the protein profile of the testes in each group. Western blotting was applied to verify the target of differentially abundant proteins (DAPs). The analysis of morphology and histology of testes showed that a certain concentration of 1α,25(OH)2VD3-glycosides had a positive and significant effect on testicular development. And the results of proteomics analysis showed that of the identified 132,715 peptides, 122,755 were unique peptides. 7852 proteins, of which 6573 proteins contain quantitative information. Screening for DAPs focused on proteins closely related to the regulation of testicular development such as steroid hormone synthesis, steroid biosynthesis, peroxisome and fatty acid metabolism pathways. These results indicated that 1α,25(OH)2VD3 is involved in the regulation of early testicular development in piglets. At the same time, these findings provide valuable information for the proteins involved in the regulation of testicular development, and help to better understand the mechanisms of 1α,25(OH)2VD3 in regulating the development of piglets’ testes.
format	article
author	Haodong Chen Kathrin Bühler Yan Zhu Xiongwei Nie Wanghong Liu
author_facet	Haodong Chen Kathrin Bühler Yan Zhu Xiongwei Nie Wanghong Liu
author_sort	Haodong Chen
title	Proteomics analysis reveals the effect of 1α,25(OH)2VD3-glycosides on development of early testes in piglets
title_short	Proteomics analysis reveals the effect of 1α,25(OH)2VD3-glycosides on development of early testes in piglets
title_full	Proteomics analysis reveals the effect of 1α,25(OH)2VD3-glycosides on development of early testes in piglets
title_fullStr	Proteomics analysis reveals the effect of 1α,25(OH)2VD3-glycosides on development of early testes in piglets
title_full_unstemmed	Proteomics analysis reveals the effect of 1α,25(OH)2VD3-glycosides on development of early testes in piglets
title_sort	proteomics analysis reveals the effect of 1α,25(oh)2vd3-glycosides on development of early testes in piglets
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/da0bd854e2ae43bb9e0f7026ba9f915e
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Proteomics analysis reveals the effect of 1α,25(OH)2VD3-glycosides on development of early testes in piglets

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