Comparative transcriptomic analysis reveals the roles of ROS scavenging genes in response to cadmium in two pak choi cultivars

Abstract To identify key regulatory genes involved in ROS scavenging in response to cadmium (Cd) exposure in pak choi, eight cDNA libraries from Cd-treated and Cd-free roots of two cultivars, Baiyewuyueman (high Cd accumulator) and Kuishan’aijiaoheiye (low Cd accumulator), were firstly performed by...

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Autores principales:	Rugang Yu, Yunshu Tang, Caifeng Liu, Xueling Du, Chunmei Miao, Gangrong Shi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/da0c10c8c9ec4bc1a36e88dae01c289c
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spelling	oai:doaj.org-article:da0c10c8c9ec4bc1a36e88dae01c289c2021-12-02T11:40:23ZComparative transcriptomic analysis reveals the roles of ROS scavenging genes in response to cadmium in two pak choi cultivars10.1038/s41598-017-09838-22045-2322https://doaj.org/article/da0c10c8c9ec4bc1a36e88dae01c289c2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-09838-2https://doaj.org/toc/2045-2322Abstract To identify key regulatory genes involved in ROS scavenging in response to cadmium (Cd) exposure in pak choi, eight cDNA libraries from Cd-treated and Cd-free roots of two cultivars, Baiyewuyueman (high Cd accumulator) and Kuishan’aijiaoheiye (low Cd accumulator), were firstly performed by RNA-sequencing. Totally 0.443 billion clean reads and 244,190 unigenes were obtained from eight transcriptome. About 797 and 1167 unigenes encoding ROS related proteins and transcription factors were identified. Of them, 11 and 16 ROS scavenging system related DEGs, and 29 and 15 transcription factors related DEGs were found in Baiyewuyueman and Kuishan’aijiaoheiye, respectively. Ten ROS-scavenging genes (Cu/Zn-SOD, GST1, PODs, TrxR2, PrxR, FER3 and NDPK) showed higher expression levels in Cd-exposed seedings of Baiyewuyueman than those of Kuishan’aijiaoheiye. Four genes (GPX, APX, GRX and GST3) specifically expressed in Cd-free roots of Kuishan’aijiaoheiye. For transcription factors, ERF12/13/22 and WRKY31 was up-regulated by Cd in Baiyewuyueman, while in Kuishan’aijiaoheiye, Cd induced down-regulations of bZIP, NAC and ZFP families. The results indicate that the two cultivars differed in the mechanism of ROS scavenging in response to Cd stress. Fe SOD1, POD A2/44/54/62 and GST1 may be responsible for the difference of Cd tolerance between Baiyewuyueman and Kuishan’aijiaoheiye.Rugang YuYunshu TangCaifeng LiuXueling DuChunmei MiaoGangrong ShiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rugang Yu Yunshu Tang Caifeng Liu Xueling Du Chunmei Miao Gangrong Shi Comparative transcriptomic analysis reveals the roles of ROS scavenging genes in response to cadmium in two pak choi cultivars
description	Abstract To identify key regulatory genes involved in ROS scavenging in response to cadmium (Cd) exposure in pak choi, eight cDNA libraries from Cd-treated and Cd-free roots of two cultivars, Baiyewuyueman (high Cd accumulator) and Kuishan’aijiaoheiye (low Cd accumulator), were firstly performed by RNA-sequencing. Totally 0.443 billion clean reads and 244,190 unigenes were obtained from eight transcriptome. About 797 and 1167 unigenes encoding ROS related proteins and transcription factors were identified. Of them, 11 and 16 ROS scavenging system related DEGs, and 29 and 15 transcription factors related DEGs were found in Baiyewuyueman and Kuishan’aijiaoheiye, respectively. Ten ROS-scavenging genes (Cu/Zn-SOD, GST1, PODs, TrxR2, PrxR, FER3 and NDPK) showed higher expression levels in Cd-exposed seedings of Baiyewuyueman than those of Kuishan’aijiaoheiye. Four genes (GPX, APX, GRX and GST3) specifically expressed in Cd-free roots of Kuishan’aijiaoheiye. For transcription factors, ERF12/13/22 and WRKY31 was up-regulated by Cd in Baiyewuyueman, while in Kuishan’aijiaoheiye, Cd induced down-regulations of bZIP, NAC and ZFP families. The results indicate that the two cultivars differed in the mechanism of ROS scavenging in response to Cd stress. Fe SOD1, POD A2/44/54/62 and GST1 may be responsible for the difference of Cd tolerance between Baiyewuyueman and Kuishan’aijiaoheiye.
format	article
author	Rugang Yu Yunshu Tang Caifeng Liu Xueling Du Chunmei Miao Gangrong Shi
author_facet	Rugang Yu Yunshu Tang Caifeng Liu Xueling Du Chunmei Miao Gangrong Shi
author_sort	Rugang Yu
title	Comparative transcriptomic analysis reveals the roles of ROS scavenging genes in response to cadmium in two pak choi cultivars
title_short	Comparative transcriptomic analysis reveals the roles of ROS scavenging genes in response to cadmium in two pak choi cultivars
title_full	Comparative transcriptomic analysis reveals the roles of ROS scavenging genes in response to cadmium in two pak choi cultivars
title_fullStr	Comparative transcriptomic analysis reveals the roles of ROS scavenging genes in response to cadmium in two pak choi cultivars
title_full_unstemmed	Comparative transcriptomic analysis reveals the roles of ROS scavenging genes in response to cadmium in two pak choi cultivars
title_sort	comparative transcriptomic analysis reveals the roles of ros scavenging genes in response to cadmium in two pak choi cultivars
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/da0c10c8c9ec4bc1a36e88dae01c289c
work_keys_str_mv	AT rugangyu comparativetranscriptomicanalysisrevealstherolesofrosscavenginggenesinresponsetocadmiumintwopakchoicultivars AT yunshutang comparativetranscriptomicanalysisrevealstherolesofrosscavenginggenesinresponsetocadmiumintwopakchoicultivars AT caifengliu comparativetranscriptomicanalysisrevealstherolesofrosscavenginggenesinresponsetocadmiumintwopakchoicultivars AT xuelingdu comparativetranscriptomicanalysisrevealstherolesofrosscavenginggenesinresponsetocadmiumintwopakchoicultivars AT chunmeimiao comparativetranscriptomicanalysisrevealstherolesofrosscavenginggenesinresponsetocadmiumintwopakchoicultivars AT gangrongshi comparativetranscriptomicanalysisrevealstherolesofrosscavenginggenesinresponsetocadmiumintwopakchoicultivars
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Comparative transcriptomic analysis reveals the roles of ROS scavenging genes in response to cadmium in two pak choi cultivars

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